How to extract restriction enzyme (or restriction endonuclease) from bacteria? - (Aug/18/2010 )

Hello People,

Is there a simple method to extract restriction enzyme from bacteria?

Thank you.

Hola, I´m not sure but I supposse that if you could , you will obtain an small amount. I think that they are obtained by cloning , probably with a tag to achieve a big amount of active pure protein. Regards