Lipid extraction from parrafin embedded tissue ? - (Jan/21/2013 )
I am planning on doing lipid analysis on cells, tissue (fresh frozen) and parrafin embedded.
Among other things I want to perform a cholesterol level measurement, is it possible to do so by lipid extraction from formalin-fixed parrafin-embedded tissue ? (Fresh frozen is probably the best but I have more material parrafin embedded)
Unfortunately for you, the standard process for paraffin embedding is to dehydrate the tissue first (paraffin is immiscible with water). This is most commonly done in a series of graded alcohols followed by xylene, which will dissolve the fats.