Lipid extraction with ORO - (Sep/21/2010 )

Hi,

I have to stain and extract lipids with the ORO method, and I was wondering if I can store the cells in water for 1 or 2 days after fixation with formaldehyde? In my opinion, there should be no problems, but if someone could confirm, it would be appreciated ^^

Thanks!

no, do not save in water. save in PBS. pH is very important. you need a buffer, with a certain neutral pH. Not always you know what your water's pH is, although formaldehyde fixation is very promising and saves cells in their natural shape.

PBS would be even better than water, for sure! Thanks for the tip.