Protocol Online logo
Top : New Forum Archives (2009-): : Cell Biology

Lipid extraction with ORO - (Sep/21/2010 )

Hi,

I have to stain and extract lipids with the ORO method, and I was wondering if I can store the cells in water for 1 or 2 days after fixation with formaldehyde? In my opinion, there should be no problems, but if someone could confirm, it would be appreciated ^^

Thanks!

-madrius1-

no, do not save in water. save in PBS. pH is very important. you need a buffer, with a certain neutral pH. Not always you know what your water's pH is, although formaldehyde fixation is very promising and saves cells in their natural shape.

-Curtis-

PBS would be even better than water, for sure! Thanks for the tip.

-madrius1-