how to extract enzyme allinase from garlic - (Sep/25/2010 )

how to extract allinase from garlic? and how to purify it?

how to determine its concentration?
im going to incubate garlic in different temperature.. how is it to determine whether there is changes in the behaviour of enzyme..

allin will interact allinase to become allicin.. do i have to use allicin to observe the reaction of the allinase?

this paper in plant physiology can give you details on purification and assay of alliinase (it was from onion but the procedure should work or you can use the references to get it for garlic):

a novel alliinase from onion roots