miRNA extraction from cell-conditioned media? - (Jan/20/2012 )
Has anyone had any success extraction miRNA from cell-conditioned media?
Theres very little reported evidence of it out there. Some have used miRNeasy and miRVana, but yields are extremely low.
I heard that in a group the have done it as 2 part method: 1st extract Mitochondria and then extract RNA from them.