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Gene Related Discussions
  1. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 5)
  2. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
  3. need some advise about PCR of PKD1-human gene - (reply: 7)
  4. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
  5. Amplification in HK gene but not for target gene - (reply: 3)
  6. orthologs of genes - (reply: 1)
  7. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
  8. Finding out the regulating transcription factors of a list of genes ? - (reply: 1)
  9. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
  10. how can i find whole exons and introns for a special gene - (reply: 8)
  11. What is the RNA:Protein ratio for ONE gene over different RNA levels? - (reply: 1)
  12. Gene knockout results in increasing mouse spleen weight and reducing % eosinophi - (reply: 1)
  13. chk effect of cell line genes on normal dna - (reply: 1)
  14. Ct value of housekeeping gene - (reply: 4)
  15. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
  16. Gene silencing and miRNA isolation - (reply: 1)
  17. Reference gene(s) selection and validaton for qPCR - (reply: 2)
  18. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
  19. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  20. If MCF-7 cell line can expresses Her-2 gene? - (reply: 5)
  21. Gene cloning with unknown sequence - (reply: 6)
  22. Reference genes in miRNAs - (reply: 3)
  23. mRNA copies of each gene under different promoters - (reply: 1)
  24. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
  25. simultaneous knock down of two genes in a single cell line - (reply: 1)
  26. Can we add a restriction site to a gene? - (reply: 1)
  27. Mouse unique genes vs human unique ones - (reply: 3)
  28. Need no RT control for all genes? - (reply: 1)
  29. Different annealing temp between HKG and target gene - (reply: 2)
  30. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  31. How to determine the size of gene to amplify? - (reply: 1)
  32. AZA treatment induces the genes but bisulfite sequencing does not reveal de-meth - (reply: 11)
  33. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  34. Can multiple genes follow only one promoter? - (reply: 1)
  35. does DNA methylation outside promoter region affect gene Transcription/expressio - (reply: 1)
  36. BLAST sequence analysis and gene identification - (reply: 2)
  37. Determining CpG methylation and effect on gene expression - (reply: 1)
  38. i couldn't get the band of target gene after ligation - (reply: 9)
  39. suitable single-copy gene target for fungal qPCR - (reply: 4)
  40. Suppression of a gene by Antisense strategy - (reply: 3)
  41. qPCR of Intronless gene - (reply: 4)
  42. High Ct values in ref gene as well as target gene - (reply: 3)
  43. qPCR reference gene testing help - (reply: 4)
  44. Fanconi anaemia, how was the FANCA gene identified? - (reply: 1)
  45. Step-by-step protocol for newbie in gene expression analysis - (reply: 3)
  46. what is gene codon optimisation. - (reply: 5)
  47. how many copies of the gene are in the genome - (reply: 1)
  48. PCR GAPDH gene - (reply: 1)
  49. Calibrator sample has zero expression of target gene. - (reply: 1)
  50. Functional analysis of microarray gene lists - (reply: 4)
  51. I don't find housekeeping genes: what should I do? - (reply: 6)
  52. Do genes always need to be cloned in the "right direction"? - (reply: 3)
  53. Gene sequence for Real time PCR - (reply: 2)
  54. What is the advantage of knockout over gene therapy in mice model in order to st - (reply: 1)
  55. confirmation of real gene status - (reply: 5)
  56. Finding functions for genes in long gene lists - (reply: 2)
  57. defining ratio of alternative spliced gene with qPCR - (reply: 14)
  58. PCR gene specific amplification problem - (reply: 3)
  59. Reference gene for normalisation - for different growth rates - (reply: 3)
  60. Sorting tools??: Oncogenes/tumor suppressor genes - (reply: 3)
  61. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
  62. Cloning synthesised genes - (reply: 1)
  63. Low Transformation Efficiency with a Specific Gene - (reply: 4)
  64. Gene of interest targeting micrornas - (reply: 6)
  65. gene cloning, where to start? - (reply: 1)
  66. Artificial RNA as normalizer (HK gene) - (reply: 1)
  67. GHR gene location of water buffalo? - (reply: 2)
  68. Insoluble Protein of Interest Resulted from Mammalian Gene Expression - Can Any - (reply: 3)
  69. Gene expression profiling with small cell number - (reply: 1)
  70. Gene expression assay with small cell number - (reply: 3)
  71. BLAST is giving me a result totally unrelated to the gene I expect - (reply: 3)
  72. Two genes, same MCS - (reply: 1)
  73. gene cloning - (reply: 1)
  74. Truncated gene Cloning - (reply: 1)
  75. Over expression of genes and their effect on Apoptosis or mitosis - (reply: 1)
  76. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
  77. His tag introduction in to gene and primer design - (reply: 2)
  78. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
  79. reference gene for qPCR in E.coli - (reply: 1)
  80. Cloning at 6 kb gene - (reply: 3)
  81. Problem amplifying viral gene - (reply: 5)
  82. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
  83. finding gene sequence to delete - (reply: 4)
  84. Designing an experiement to identify mutations in particular genes - (reply: 5)
  85. heterologous gene integration into e.coli genome - (reply: 1)
  86. Finding promoters and genes - (reply: 2)
  87. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
  88. Validation of Up-regulated genes - (reply: 1)
  89. how to find out the DNA seuence of a human gene from online database? - (reply: 1)
  90. Gene expression database - (reply: 1)
  91. How base pairs upstream of gene to include for the promoter? - (reply: 3)
  92. PCR-screening of T-DNA inserted in Arabidopsis gene - (reply: 1)
  93. Change in Superscript lot influences transcript level of one gene but not others - (reply: 2)
  94. Gene knockdown expression levels shRNAmirs - (reply: 2)
  95. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
  96. New gene discovery - (reply: 1)
  97. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
  98. converting protein names protein ids and enterz gene ids - (reply: 1)
  99. Transfection of a non human gene into human cell line - (reply: 1)
  100. looking for a difficult gene - (reply: 1)
  101. Normalization to Standard Gene: what does this mean - (reply: 8)
  102. Tagging a gene with HA and FLAG using PCR - (reply: 1)
  103. Directions of genes expression illustrations - (reply: 5)
  104. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  105. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
  106. Which one is better as reference gene base on these data - (reply: 3)
  107. Restriction enzymes for a gene - (reply: 2)
  108. Better option, mutagenesis or gene synthesis? - (reply: 4)
  109. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  110. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
  111. qPCR: CT value of reference gene - (reply: 3)
  112. cloning a gene in to a plasmid - (reply: 5)
  113. Which cells express which genes? - (reply: 1)
  114. Looking for tool that searches a list of genes for common MiRs - (reply: 1)
  115. How to find promoter sequence of a gene - (reply: 1)
  116. Heterologous gene expression in yeast - a bioinformatician needs your help cloni - (reply: 1)
  117. geNorm alternative for qPCR housekeeping genes - (reply: 1)
  118. Pcr product size determination for a fusion gene - (reply: 8)
  119. How to download whole Genome of Bacteria with Individual genes in FASTA format a - (reply: 3)
  120. validation of microRNA target gene - (reply: 6)
  121. Textbook Canon: Expressing Any Genes in Bacteria - (reply: 21)
  122. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
  123. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
  124. Alternative to gene silencing for functional assay?? much help needed :) - (reply: 1)
  125. Taqman assay for detecting expression of genes carrying specific SNPs - (reply: 2)
  126. Trick for making diagram of gene for paper figure? - (reply: 4)
  127. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
  128. Best way to genotpy a mutant gene with a 1bp difference. - (reply: 3)
  129. A good vector to make gene library? - (reply: 5)
  130. SNP genotyping of whole gene - (reply: 4)
  131. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  132. Receptor and gene interaction - (reply: 2)
  133. Transformation of an inducible GFP gene into Aspergillus niger - (reply: 2)
  134. Gene expression - (reply: 5)
  135. Help with CGL gene cloning problems - (reply: 1)
  136. sequencing result doesn't match my gene.... - (reply: 5)
  137. How to get pure protein of a gene - (reply: 1)
  138. How to get pure protein of a gene - (reply: 3)
  139. How to insert genes into chromosome of E. coli - (reply: 1)
  140. Homologous Recombination of 2 genes with greatly different lenghts?. - (reply: 2)
  141. Spliced and unspliced form of a gene - (reply: 3)
  142. Gene synthesis - (reply: 2)
  143. cloning of a long gene using cDNA - (reply: 1)
  144. Finding region of interest in a gene - (reply: 2)
  145. Isolation of a Gene of Interest for Subcloning - (reply: 8)
  146. books for bacteria gene knockout - (reply: 1)
  147. books about bacteria gene knockout - (reply: 9)
  148. Functional relationships between genes that are contiguous in the genome - (reply: 2)
  149. Too low Ct value for reference gene !!! - (reply: 3)
  150. Problem with expressing genes using tac promoter - (reply: 4)
  151. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
  152. Gene expression - (reply: 1)
  153. DNA Construction: HIV-1 Tat Gene - (reply: 9)
  154. qPCR normalization of reference gene - (reply: 1)
  155. inquiry about the methods for gene knockout in bacteria - (reply: 1)
  156. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
  157. housekeeping gene has not uniform pattern - (reply: 3)
  158. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  159. cloning an AT rich gene - (reply: 3)
  160. Cloning two genes in the same plasmid - (reply: 4)
  161. RNAi problem - Gene is knocked down, but not protein - (reply: 8)
  162. relative qPCR analysis when gene is not expressed at baseline - (reply: 8)
  163. Any free software for drawing genes? - (reply: 9)
  164. Plasmid, gene orientation, insert orientation. - (reply: 1)
  165. good tutorials/protocols/books on gene cloning - (reply: 2)
  166. Determining gene of interest from Cell culture lines - (reply: 2)
  167. compare between 2 or more genes - (reply: 1)
  168. Knocking out non-essential gene - (reply: 2)
  169. Double digest after cloning doesn't give gene - (reply: 6)
  170. How much of plasmid should be cut to see the gene - (reply: 1)
  171. Comparison of gene structure - (reply: 1)
  172. HaCaT reference gene - (reply: 1)
  173. checking whther there is any miRNA in introns of a specific gene. - (reply: 1)
  174. Need help for PCR for AT rich gene - (reply: 3)
  175. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  176. Relative quantification of different genes in single sample - same threshold val - (reply: 1)
  177. How to find the peptide fragment of target gene? - (reply: 1)
  178. gene expression in E coli with PET21a vector - (reply: 1)
  179. how to find gene sequences online (need both exons and introns) - (reply: 3)
  180. websits for gene exon and intron sequences - (reply: 1)
  181. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
  182. Cloning promoter (overlap with another gene!) - (reply: 3)
  183. how to know introns of a gene - (reply: 3)
  184. methods for gene sequencing - (reply: 4)
  185. expression of dirrefent isoforms f the same gene in different tissues - (reply: 1)
  186. How do I find the promoter of a gene? - (reply: 3)
  187. How to define a promoter region of a gene? - (reply: 1)
  188. Housekeeping gene for COS-1 cells - (reply: 1)
  189. Low Molecular Weight Housekeeping Gene for Loading Control? - (reply: 2)
  190. Identifying which promoters are regulating gene expression - (reply: 2)
  191. Cell lines & gene of interest - (reply: 1)
  192. Software for selection genes related to concrete cell processes - (reply: 1)
  193. a gene expressed in 293 cell lines but not Hela cells - (reply: 1)
  194. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)
  195. How genes are selectively silenced ? - (reply: 1)
  196. real time qRT-PCR and low expression gene - (reply: 2)
  197. Cloning genes into plant vector - (reply: 1)
  198. PCRing mature form of a gene - (reply: 1)
  199. Gene Expression Issues - (reply: 3)
  200. No gene amplification, 18s amplification is fine - (reply: 1)
  201. Cloning large gene - (reply: 3)
  202. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  203. Confirmed gene knockdown not showing on array - (reply: 2)
  204. how to determine fold change of gene expression - (reply: 2)
  205. negative values after GOI - reference gene ?? - (reply: 4)
  206. Gene expression change after mutagenesis-- please help! - (reply: 4)
  207. Two start codons" the TAG and the gene" - (reply: 1)
  208. expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
  209. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
  210. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
  211. Gene on negative strand - two eductional questions. - (reply: 3)
  212. 3'UTR sequence of a gene - (reply: 3)
  213. Enriched gene signatures - (reply: 1)
  214. how does the binding of miRNA at 3'UTR regulates the gene - (reply: 3)
  215. Sigma esiRNA induces target gene expression - (reply: 1)
  216. High resolution melting to gene scanning - (reply: 1)
  217. Is one reference gene ever appropriate? - (reply: 3)
  218. Trouble to UNDERSTAND gene replacement results - (reply: 1)
  219. Comprehensive database for all the known epigenetic genes in human and mouse - (reply: 2)
  220. Restriction Enzyme sites in my genes - (reply: 1)
  221. infection with two retroviruses? - Delivery of two Genes in distinct virus constructs, does it work? (reply: 3)
  222. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  223. qPCR with low expressing genes - (reply: 1)
  224. Reference genes. - (reply: 1)
  225. Reference genes - (reply: 2)
  226. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
  227. Problem cloning wild type E. coli gene... Possible recombination event in DH5alp - (reply: 4)
  228. Gene set similarity searches in public databases - (reply: 4)
  229. Gene copy number analysis - (reply: 1)
  230. reference gene - (reply: 2)
  231. Predicting function of heterozygous vs WT gene? - (reply: 1)
  232. is there a method to set up stable transfectants without drug selection - no drug-resistant gene (reply: 8)
  233. Selection of housekeeping gene in Mycobacterium - (reply: 1)
  234. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
  235. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
  236. finding exons for a gene, having the coding sequence only - (reply: 4)
  237. New to Gene cloning - Plasmid vector preparation (reply: 2)
  238. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  239. Regression analysis between two genes - (reply: 1)
  240. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
  241. copy number of gene in genome - (reply: 4)
  242. Cloning of a gene containing 31 exon - (reply: 1)
  243. predict union between microRNAs and genes - (reply: 2)
  244. A gene is not inserted into the Plasmid - (reply: 2)
  245. Reporter gene to test new promotor - (reply: 1)
  246. How to study with the candidate genes? - (reply: 2)
  247. Delta Delta Ct method for multiple reference genes? - (reply: 1)
  248. Cloning a foreign gene into E-coli - (reply: 1)
  249. virus with human gene to mouse cell - Does it work? (reply: 2)
  250. Transfecting a cancer gene - (reply: 2)
  251. housekeeping genes in PCR - (reply: 1)
  252. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
  253. gene expressional analysis - (reply: 1)
  254. Biotinylation sequence: gene orientation?? - (reply: 1)
  255. Can every gene be methylated? - (reply: 1)
  256. Antibody gene cloning - (reply: 3)
  257. Gene not expressed in wild-type and knockout yet differences in microarray - (reply: 15)
  258. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  259. Gene targeting - (reply: 3)
  260. Cheap procedure for gene deletion in E coli? - (reply: 2)
  261. integration of a gene per homologous recombination - (reply: 5)
  262. Ki-67 - no difference bwteen tumor suppressor gene vs ctrl (reply: 3)
  263. relative comparison of many samples and genes in qRT-PCR - (reply: 1)
  264. Reference genes and dilutions in qPCR - (reply: 1)
  265. Molecular identification of ESBL genes blaGES-1, blaVEB-1, blaCTX-M blaOXA-1, bl - International Journal Biological & Life Sciences. 6 (3): 138-142, (reply: 1)
  266. [Software] Drawing arrows as ORFs on genes - (reply: 5)
  267. cre-loxP: how to check if the gene is expressed - (reply: 3)
  268. cre-loxP: how to check if the gene is expressed - (reply: 8)
  269. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
  270. Two genes that are contiguous in the genome... - ...the relationship between them (reply: 7)
  271. clone GENE RT-PCT PRIMERS - cloning genes (reply: 1)
  272. Ghost gene - (reply: 3)
  273. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  274. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
  275. trouble with protein expression of high AT rich gene - (reply: 1)
  276. Cytosolic Housekeeping gene - (reply: 1)
  277. Gene signatures in breast cancer - (reply: 2)
  278. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  279. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
  280. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
  281. How to download the complete list of genes in a chromosome(chromosome 19) - finding complete list of genes in a chomosome in NCBI map viewer (reply: 7)
  282. help with ct of reference gene - (reply: 1)
  283. colony PCR for subcloning gene - (reply: 1)
  284. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  285. siRNA sequence can be used as shRNA? - Stable down regulation of target gene expression. (reply: 1)
  286. how long will it takes to silence target gene after induction of shRNA - (reply: 2)
  287. how to find kappaB binding site on the promoter of a gene - kappaB binding site in gene promoter (reply: 2)
  288. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
  289. Links between genes and risk factors in polygenic diseases - (reply: 2)
  290. Does a viral gene have introns? - (reply: 4)
  291. Blast Nucleotide Issue - No more Gene ID when comparing Bacteria genome (reply: 3)
  292. Gene expression confusion - (reply: 5)
  293. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  294. use of housekeeping gene in RT PCR chIP - (reply: 3)
  295. Gene cross-checking - (reply: 14)
  296. Using Gene Ontology? - (reply: 1)
  297. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
  298. High Gene low protein levels - (reply: 1)
  299. stable clones - correlation between 2 genes in the same family (reply: 1)
  300. how to find TSS of a certain gene - (reply: 1)
  301. How to get a list of all variants (SNPs) within a gene and 5kb immediately 5' - (reply: 3)
  302. About gene orientation - (reply: 2)
  303. very high and inconsistent Cts for the house keeping gene - (reply: 4)
  304. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
  305. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
  306. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
  307. Looking for FASTA format of DNA of a gene amongst various species - (reply: 7)
  308. House keeping gene question? - Thanks for any help! (reply: 1)
  309. Problems with validating primers and low expression genes - (reply: 3)
  310. Deep sequencing to find gene targets of protein? - (reply: 1)
  311. How can multiple cloning sites (MCS) NOT disrupt lacZ gene? - (reply: 2)
  312. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
  313. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
  314. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
  315. Gene expression of methylated gene - (reply: 4)
  316. GAPDH CT lower than target gene CT - (reply: 2)
  317. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
  318. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
  319. mutagenize plasmid-encoded gene - (reply: 4)
  320. Sequencing of gene promoter/terminator, when you only have the AA sequence of th - (reply: 2)
  321. Fusion of reporter genes - (reply: 2)
  322. Cloning from cDNA - the wrong gene?! - (reply: 2)
  323. failure PCR amplification from low GC content gene - (reply: 5)
  324. How many copies of the gene - (reply: 1)
  325. Antibiotic resistance genes in a plasmid - How are they expressed? (reply: 3)
  326. Measuring gene expression across cell lines - (reply: 2)
  327. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
  328. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
  329. How to find gene homologues? - (reply: 1)
  330. microRNAs and mRNAs. Interpreting gene expression profiles - Gene expression profiling and microRNAs (reply: 4)
  331. complete gene for expression? - (reply: 2)
  332. questions on gene therapy in brain - (reply: 1)
  333. 5' RACE for very GC-rich gene - (reply: 2)
  334. How to remove copy of redundant cDNA in a mixture - RT results in a cDNA library with high copy number of few genes (reply: 3)
  335. Large Difference between Ct of 18s rRNA and Target Gene - (reply: 2)
  336. Isoforms of a gene!! - how to find all isoforms of a gene using bioinfo tools?! (reply: 2)
  337. cloning - how to clone a gene having stop codons (reply: 4)
  338. how to intentionally alter gene sequence - (reply: 1)
  339. Relatively unknown gene, need approach suggestions - (reply: 1)
  340. Why is the y-axis labeled 'relative gene expression'? - (reply: 5)
  341. how to express the gene in opposite transcriptional direction - (reply: 3)
  342. How could I express two genes in opposite orientation - (reply: 2)
  343. always run internal control for different genes on the differnet plates? - (reply: 8)
  344. Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable (reply: 1)
  345. help needed: PCR a gene from genomic DNA - (reply: 2)
  346. cloning of cellulose synthase gene - (reply: 1)
  347. Absolute quantification and normalization - Are reference genes necessary? (reply: 1)
  348. Where can you get the sequence of one exon of a gene? - (reply: 3)
  349. Validation of housekeeping gene expression - (reply: 3)
  350. GMO's - introduction of herbicide resistance gene (reply: 3)
  351. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
  352. software for gene ontology - (reply: 2)
  353. Rotor Gene 3000 and Rotor Gene Q - (reply: 1)
  354. designing primers for genes not sequenced yet - (reply: 2)
  355. Expression of a possible toxic gene? - (reply: 5)
  356. get data from gene chip - (reply: 2)
  357. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  358. How to compare different expression levels between genes - (reply: 1)
  359. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  360. miRNA gene UTRs? - (reply: 3)
  361. reference genes for qPCR - (reply: 3)
  362. Q about the housekeeping gene - (reply: 1)
  363. deleting a certain gene from bacterium - (reply: 12)
  364. promoter of a gene - (reply: 2)
  365. searching housekeeping genes - (reply: 4)
  366. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
  367. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
  368. gene copy number calculation - qPCR (reply: 2)
  369. Reference gene qPCR - (reply: 4)
  370. doubt in gene annotation - doubt in submitting gene annotation to NCBI (reply: 1)
  371. relative gene expression with multiple reference genes - data analysis (reply: 1)
  372. How to detect a gene having multiple isoforms using southern/northen techniques - (reply: 1)
  373. What Factors Affect Ct values of a gene? - (reply: 1)
  374. Can linear vectors be transformed and can they express resistance genes? - (reply: 4)
  375. how to test if a given microRNA regulates a gene of interest - microrna target (reply: 7)
  376. how to know a sequene for a specific gene & produce it - (reply: 5)
  377. Gene insertion without desired unique restriction sites - (reply: 3)
  378. Gene cluster drawing software - Anythign to draw genes cluterested together like in an operon (reply: 1)
  379. How to find upstream genomic sequence of a gene? - (reply: 2)
  380. Gene expressed in lenti-packaging cells, but not in lenti-infected cells - (reply: 2)
  381. Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene - (reply: 2)
  382. getting crazy about a gene bank entry - (reply: 2)
  383. Need Help: Bacterial gene deletion- sacB sucrose resistance - (reply: 31)
  384. Problems with gene knockdown - No knockdown with validated shRNAs :( (reply: 6)
  385. problem in gene cloning - (reply: 4)
  386. Trouble with GAPDH housekeeping gene - (reply: 3)
  387. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
  388. HELP: gene with intron expressed in Pichia - (reply: 2)
  389. Promoter/gene cloning question - (reply: 7)
  390. Gene Ontology - Looking for a good software (reply: 3)
  391. Gene screening - (reply: 2)
  392. Gene Expression analysis - (reply: 4)
  393. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  394. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
  395. troubleshooting: gene deletions by method of Wanner and Datsenko - (reply: 2)
  396. yeast transformation - Expression of plant genes in yeast (reply: 1)
  397. Real time house keeping genes change - (reply: 1)
  398. A MAPKKK gene, receptor or not? - (reply: 3)
  399. What genes can be used to validate H3K27me3-ChIP - (reply: 8)
  400. Tool for design gene structure figures - (reply: 5)
  401. how to determine certain gene copy number? - (reply: 3)
  402. expressing data with more than 1 HK gene - (reply: 2)
  403. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
  404. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  405. known gene or area of a chromosome - help (reply: 1)
  406. Amplifying a gene using a degenerate primer - (reply: 4)
  407. Chosing the best housekeeping gene! - (reply: 5)
  408. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
  409. need help on isolation of intron of gene - (reply: 1)
  410. SiRNA- gene silencing in brain Organotypic slice cultures - (reply: 3)
  411. Gene expression in cell line - (reply: 1)
  412. Is H3K4 trimethylation always associated with gene activation? - (reply: 1)
  413. house keeping gene in transgenic mice. - (reply: 1)
  414. Detect gene in stable cell line, - (reply: 4)
  415. selection of internal control gene - (reply: 2)
  416. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
  417. GFP reporter gene - (reply: 3)
  418. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
  419. Bacteria gene expression - (reply: 2)
  420. minimum length for the gene to be amplified in PCR - (reply: 6)
  421. wrong gene - (reply: 13)
  422. gene design - restriction site addition (reply: 2)
  423. Number of genes and number of proteins in humans - (reply: 1)
  424. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
  425. Understanding qPCR and gene expression changes - (reply: 10)
  426. zebrafish gene regulation can I use mammel expression vector? - (reply: 1)
  427. Heterogenous expresion of stably transfected gene - (reply: 4)
  428. assembling problem in genes - assembling problem in genes (reply: 7)
  429. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  430. Promoters and gene expression - (reply: 3)
  431. Automated sequencing - Difficult gene (reply: 1)
  432. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  433. Gene knockin or knockout? transgenic mouse - (reply: 2)
  434. want gene knock out protocol - (reply: 2)
  435. Target gene regulation by transiently transfected PR - (reply: 4)
  436. Mycobacterial housekeeping genes - (reply: 1)
  437. Transfer of gene from one vector to another vector - (reply: 2)
  438. Macroarray platforms - CGH vs Gene Expression (reply: 4)
  439. Houskeeping Gene not steady - (reply: 7)
  440. candidate gene approaches - (reply: 3)
  441. size of gene insert wrong? - (reply: 3)
  442. size of gene insert vs. predicted size - (reply: 1)
  443. Good SiRNA Gene silencing - (reply: 1)
  444. yeast gene deletion - Could I delete an essential gene from haploid cells? (reply: 8)
  445. deleting lacZ gene from control vector - (reply: 5)
  446. methylation by MSSS1(NEB )for reporter gene analysis - (reply: 2)
  447. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  448. RNAi in bacteria - Why no finding for gene silencing in bacteria? (reply: 1)
  449. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)