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Gene Related Discussions
  1. CRISPR-Cas9 / insert a gene in cell line genome - (reply: 1)
  2. PCR positive control of antibiotics resistance gene - (reply: 1)
  3. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
  4. gene expression in cultured cell lines - (reply: 1)
  5. Map Viewer for several genes - (reply: 1)
  6. Why mutations in different genes cause the same disease across different familie - (reply: 3)
  7. Target gene cloning - (reply: 4)
  8. What are the different methods to inhibit a gene in an organism? - (reply: 1)
  9. Enhancer Identification for Specific Genes - (reply: 3)
  10. What gene to insert in my anti-cancer virus? - (reply: 2)
  11. Gene expression heat map ? - (reply: 1)
  12. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
  13. Cloned gene in pET system not being expressed - (reply: 10)
  14. Question regarding protein conservation among similar genes - (reply: 2)
  15. Problem with subcloning genescript synthesized genes - (reply: 2)
  16. Housekeeping genes - Cq values - (reply: 4)
  17. Can siRNA be used to prevent upregulation in gene expression? - (reply: 3)
  18. Jurkat cell - normal receptor/gene expressivity - (reply: 1)
  19. Using Gene Ontology - (reply: 2)
  20. Inactivating effects on immune cells caused by mutation in the gene encoding the - (reply: 2)
  21. How to identify gene based on molecular weight? - (reply: 4)
  22. Why is the "gene" the smallest unit of heredity? - (reply: 1)
  23. Help Finding Genes In Other Species (Entrez and SPLIGN) - (reply: 1)
  24. qPCR with different genes - (reply: 5)
  25. How to determine gene knock-out from Agarose gel bands - (reply: 1)
  26. Cloning of an unknown gene - (reply: 3)
  27. Cloning of an unknown gene - (reply: 3)
  28. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
  29. How to select promoter for resistance gene in linear/naked DNA "knockout" - (reply: 2)
  30. Cross-species genes on the chromosomes - (reply: 4)
  31. Sequence for nucleotide 845 in HFE gene - (reply: 3)
  32. low copy number housekeeping genes - (reply: 2)
  33. Reference Gene/HKG- every plate - (reply: 2)
  34. qPCR standard for lipase genes - (reply: 2)
  35. Campylobacter jejuni qPCR targeting VS1 gene - (reply: 2)
  36. Gene expression and transcription factors - (reply: 1)
  37. When is it suitable for using NIH3T3 cells to validate cancer genes? - (reply: 1)
  38. How do i validate a cancer gene? - (reply: 3)
  39. problem in sequencing of gene - (reply: 3)
  40. TaqMan gene expression assays - (reply: 1)
  41. primer design for a gene - (reply: 3)
  42. Problems regarding amplification of my gene - (reply: 3)
  43. oncolytic viruses: do they cause oncolytic activity due to excessive gene expres - (reply: 3)
  44. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
  45. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
  46. need some advise about PCR of PKD1-human gene - (reply: 7)
  47. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
  48. Amplification in HK gene but not for target gene - (reply: 3)
  49. orthologs of genes - (reply: 1)
  50. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
  51. Finding out the regulating transcription factors of a list of genes ? - (reply: 1)
  52. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
  53. how can i find whole exons and introns for a special gene - (reply: 8)
  54. KIR gene promoters for MSP primers design - (reply: 2)
  55. What is the RNA:Protein ratio for ONE gene over different RNA levels? - (reply: 1)
  56. Gene knockout results in increasing mouse spleen weight and reducing % eosinophi - (reply: 1)
  57. chk effect of cell line genes on normal dna - (reply: 1)
  58. Ct value of housekeeping gene - (reply: 4)
  59. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
  60. Gene silencing and miRNA isolation - (reply: 1)
  61. Reference gene(s) selection and validaton for qPCR - (reply: 5)
  62. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
  63. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  64. If MCF-7 cell line can expresses Her-2 gene? - (reply: 5)
  65. Gene cloning with unknown sequence - (reply: 6)
  66. Reference genes in miRNAs - (reply: 3)
  67. mRNA copies of each gene under different promoters - (reply: 1)
  68. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
  69. simultaneous knock down of two genes in a single cell line - (reply: 1)
  70. Can we add a restriction site to a gene? - (reply: 1)
  71. Mouse unique genes vs human unique ones - (reply: 3)
  72. Need no RT control for all genes? - (reply: 1)
  73. Different annealing temp between HKG and target gene - (reply: 2)
  74. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  75. How to determine the size of gene to amplify? - (reply: 1)
  76. AZA treatment induces the genes but bisulfite sequencing does not reveal de-meth - (reply: 11)
  77. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  78. How to qualitatively measure DNA methylation of gene promoter - (reply: 1)
  79. Can multiple genes follow only one promoter? - (reply: 1)
  80. does DNA methylation outside promoter region affect gene Transcription/expressio - (reply: 1)
  81. BLAST sequence analysis and gene identification - (reply: 2)
  82. Determining CpG methylation and effect on gene expression - (reply: 1)
  83. i couldn't get the band of target gene after ligation - (reply: 9)
  84. suitable single-copy gene target for fungal qPCR - (reply: 4)
  85. Suppression of a gene by Antisense strategy - (reply: 3)
  86. qPCR of Intronless gene - (reply: 4)
  87. High Ct values in ref gene as well as target gene - (reply: 3)
  88. qPCR reference gene testing help - (reply: 4)
  89. Fanconi anaemia, how was the FANCA gene identified? - (reply: 1)
  90. Step-by-step protocol for newbie in gene expression analysis - (reply: 3)
  91. what is gene codon optimisation. - (reply: 5)
  92. how many copies of the gene are in the genome - (reply: 1)
  93. PCR GAPDH gene - (reply: 1)
  94. Calibrator sample has zero expression of target gene. - (reply: 1)
  95. Functional analysis of microarray gene lists - (reply: 4)
  96. I don't find housekeeping genes: what should I do? - (reply: 6)
  97. Do genes always need to be cloned in the "right direction"? - (reply: 3)
  98. Gene sequence for Real time PCR - (reply: 2)
  99. What is the advantage of knockout over gene therapy in mice model in order to st - (reply: 1)
  100. confirmation of real gene status - (reply: 5)
  101. Finding functions for genes in long gene lists - (reply: 2)
  102. defining ratio of alternative spliced gene with qPCR - (reply: 14)
  103. PCR gene specific amplification problem - (reply: 3)
  104. Reference gene for normalisation - for different growth rates - (reply: 3)
  105. Sorting tools??: Oncogenes/tumor suppressor genes - (reply: 3)
  106. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
  107. Cloning synthesised genes - (reply: 1)
  108. Low Transformation Efficiency with a Specific Gene - (reply: 4)
  109. Gene of interest targeting micrornas - (reply: 6)
  110. gene cloning, where to start? - (reply: 1)
  111. Artificial RNA as normalizer (HK gene) - (reply: 1)
  112. GHR gene location of water buffalo? - (reply: 2)
  113. Insoluble Protein of Interest Resulted from Mammalian Gene Expression - Can Any - (reply: 3)
  114. Gene expression profiling with small cell number - (reply: 1)
  115. Gene expression assay with small cell number - (reply: 3)
  116. BLAST is giving me a result totally unrelated to the gene I expect - (reply: 3)
  117. Two genes, same MCS - (reply: 1)
  118. gene cloning - (reply: 1)
  119. Truncated gene Cloning - (reply: 1)
  120. Over expression of genes and their effect on Apoptosis or mitosis - (reply: 1)
  121. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
  122. His tag introduction in to gene and primer design - (reply: 2)
  123. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
  124. reference gene for qPCR in E.coli - (reply: 2)
  125. Cloning at 6 kb gene - (reply: 3)
  126. Problem amplifying viral gene - (reply: 5)
  127. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
  128. finding gene sequence to delete - (reply: 4)
  129. Designing an experiement to identify mutations in particular genes - (reply: 5)
  130. heterologous gene integration into e.coli genome - (reply: 1)
  131. Finding promoters and genes - (reply: 2)
  132. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
  133. Validation of Up-regulated genes - (reply: 1)
  134. how to find out the DNA seuence of a human gene from online database? - (reply: 1)
  135. Gene expression database - (reply: 1)
  136. How base pairs upstream of gene to include for the promoter? - (reply: 3)
  137. PCR-screening of T-DNA inserted in Arabidopsis gene - (reply: 1)
  138. Change in Superscript lot influences transcript level of one gene but not others - (reply: 2)
  139. Gene knockdown expression levels shRNAmirs - (reply: 2)
  140. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
  141. New gene discovery - (reply: 1)
  142. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
  143. converting protein names protein ids and enterz gene ids - (reply: 1)
  144. Transfection of a non human gene into human cell line - (reply: 1)
  145. looking for a difficult gene - (reply: 1)
  146. Normalization to Standard Gene: what does this mean - (reply: 8)
  147. Tagging a gene with HA and FLAG using PCR - (reply: 1)
  148. Directions of genes expression illustrations - (reply: 5)
  149. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  150. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
  151. Which one is better as reference gene base on these data - (reply: 3)
  152. Restriction enzymes for a gene - (reply: 2)
  153. Better option, mutagenesis or gene synthesis? - (reply: 4)
  154. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  155. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
  156. qPCR: CT value of reference gene - (reply: 3)
  157. cloning a gene in to a plasmid - (reply: 5)
  158. Which cells express which genes? - (reply: 1)
  159. Looking for tool that searches a list of genes for common MiRs - (reply: 1)
  160. How to find promoter sequence of a gene - (reply: 1)
  161. Heterologous gene expression in yeast - a bioinformatician needs your help cloni - (reply: 1)
  162. geNorm alternative for qPCR housekeeping genes - (reply: 1)
  163. Pcr product size determination for a fusion gene - (reply: 8)
  164. How to download whole Genome of Bacteria with Individual genes in FASTA format a - (reply: 3)
  165. validation of microRNA target gene - (reply: 6)
  166. Textbook Canon: Expressing Any Genes in Bacteria - (reply: 21)
  167. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
  168. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
  169. Alternative to gene silencing for functional assay?? much help needed :) - (reply: 1)
  170. Taqman assay for detecting expression of genes carrying specific SNPs - (reply: 2)
  171. Trick for making diagram of gene for paper figure? - (reply: 4)
  172. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
  173. Best way to genotpy a mutant gene with a 1bp difference. - (reply: 3)
  174. A good vector to make gene library? - (reply: 5)
  175. SNP genotyping of whole gene - (reply: 4)
  176. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  177. Receptor and gene interaction - (reply: 2)
  178. Transformation of an inducible GFP gene into Aspergillus niger - (reply: 2)
  179. Gene expression - (reply: 5)
  180. Help with CGL gene cloning problems - (reply: 1)
  181. sequencing result doesn't match my gene.... - (reply: 5)
  182. How to get pure protein of a gene - (reply: 1)
  183. How to get pure protein of a gene - (reply: 3)
  184. How to insert genes into chromosome of E. coli - (reply: 1)
  185. Homologous Recombination of 2 genes with greatly different lenghts?. - (reply: 2)
  186. Spliced and unspliced form of a gene - (reply: 3)
  187. Gene synthesis - (reply: 2)
  188. cloning of a long gene using cDNA - (reply: 1)
  189. Finding region of interest in a gene - (reply: 2)
  190. Isolation of a Gene of Interest for Subcloning - (reply: 8)
  191. books for bacteria gene knockout - (reply: 1)
  192. books about bacteria gene knockout - (reply: 9)
  193. Functional relationships between genes that are contiguous in the genome - (reply: 2)
  194. Too low Ct value for reference gene !!! - (reply: 3)
  195. Problem with expressing genes using tac promoter - (reply: 4)
  196. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
  197. Gene expression - (reply: 1)
  198. DNA Construction: HIV-1 Tat Gene - (reply: 9)
  199. qPCR normalization of reference gene - (reply: 1)
  200. inquiry about the methods for gene knockout in bacteria - (reply: 1)
  201. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
  202. housekeeping gene has not uniform pattern - (reply: 3)
  203. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  204. cloning an AT rich gene - (reply: 3)
  205. Cloning two genes in the same plasmid - (reply: 4)
  206. RNAi problem - Gene is knocked down, but not protein - (reply: 9)
  207. relative qPCR analysis when gene is not expressed at baseline - (reply: 8)
  208. Any free software for drawing genes? - (reply: 9)
  209. Plasmid, gene orientation, insert orientation. - (reply: 1)
  210. good tutorials/protocols/books on gene cloning - (reply: 2)
  211. Determining gene of interest from Cell culture lines - (reply: 2)
  212. compare between 2 or more genes - (reply: 1)
  213. Knocking out non-essential gene - (reply: 2)
  214. Double digest after cloning doesn't give gene - (reply: 6)
  215. How much of plasmid should be cut to see the gene - (reply: 1)
  216. Comparison of gene structure - (reply: 1)
  217. HaCaT reference gene - (reply: 1)
  218. checking whther there is any miRNA in introns of a specific gene. - (reply: 1)
  219. Need help for PCR for AT rich gene - (reply: 3)
  220. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  221. Relative quantification of different genes in single sample - same threshold val - (reply: 1)
  222. How to find the peptide fragment of target gene? - (reply: 1)
  223. gene expression in E coli with PET21a vector - (reply: 1)
  224. how to find gene sequences online (need both exons and introns) - (reply: 3)
  225. websits for gene exon and intron sequences - (reply: 1)
  226. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
  227. Cloning promoter (overlap with another gene!) - (reply: 3)
  228. how to know introns of a gene - (reply: 3)
  229. methods for gene sequencing - (reply: 4)
  230. expression of dirrefent isoforms f the same gene in different tissues - (reply: 1)
  231. How do I find the promoter of a gene? - (reply: 3)
  232. How to define a promoter region of a gene? - (reply: 1)
  233. Housekeeping gene for COS-1 cells - (reply: 1)
  234. Low Molecular Weight Housekeeping Gene for Loading Control? - (reply: 2)
  235. Identifying which promoters are regulating gene expression - (reply: 2)
  236. Cell lines & gene of interest - (reply: 1)
  237. Software for selection genes related to concrete cell processes - (reply: 1)
  238. a gene expressed in 293 cell lines but not Hela cells - (reply: 1)
  239. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)
  240. How genes are selectively silenced ? - (reply: 1)
  241. real time qRT-PCR and low expression gene - (reply: 2)
  242. Cloning genes into plant vector - (reply: 1)
  243. PCRing mature form of a gene - (reply: 1)
  244. Gene Expression Issues - (reply: 3)
  245. No gene amplification, 18s amplification is fine - (reply: 1)
  246. Cloning large gene - (reply: 3)
  247. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  248. Confirmed gene knockdown not showing on array - (reply: 2)
  249. how to determine fold change of gene expression - (reply: 2)
  250. negative values after GOI - reference gene ?? - (reply: 4)
  251. Gene expression change after mutagenesis-- please help! - (reply: 4)
  252. Two start codons" the TAG and the gene" - (reply: 1)
  253. expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
  254. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
  255. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
  256. Gene on negative strand - two eductional questions. - (reply: 3)
  257. 3'UTR sequence of a gene - (reply: 3)
  258. Enriched gene signatures - (reply: 1)
  259. how does the binding of miRNA at 3'UTR regulates the gene - (reply: 3)
  260. Sigma esiRNA induces target gene expression - (reply: 1)
  261. High resolution melting to gene scanning - (reply: 1)
  262. Is one reference gene ever appropriate? - (reply: 3)
  263. Trouble to UNDERSTAND gene replacement results - (reply: 1)
  264. Comprehensive database for all the known epigenetic genes in human and mouse - (reply: 2)
  265. Restriction Enzyme sites in my genes - (reply: 1)
  266. infection with two retroviruses? - Delivery of two Genes in distinct virus constructs, does it work? (reply: 3)
  267. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  268. qPCR with low expressing genes - (reply: 1)
  269. Reference genes. - (reply: 1)
  270. Reference genes - (reply: 2)
  271. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
  272. Problem cloning wild type E. coli gene... Possible recombination event in DH5alp - (reply: 4)
  273. Gene set similarity searches in public databases - (reply: 4)
  274. Gene copy number analysis - (reply: 1)
  275. reference gene - (reply: 2)
  276. Predicting function of heterozygous vs WT gene? - (reply: 1)
  277. is there a method to set up stable transfectants without drug selection - no drug-resistant gene (reply: 8)
  278. Selection of housekeeping gene in Mycobacterium - (reply: 1)
  279. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
  280. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
  281. finding exons for a gene, having the coding sequence only - (reply: 4)
  282. New to Gene cloning - Plasmid vector preparation (reply: 2)
  283. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  284. Regression analysis between two genes - (reply: 1)
  285. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
  286. copy number of gene in genome - (reply: 4)
  287. Cloning of a gene containing 31 exon - (reply: 1)
  288. predict union between microRNAs and genes - (reply: 2)
  289. A gene is not inserted into the Plasmid - (reply: 2)
  290. Reporter gene to test new promotor - (reply: 1)
  291. How to study with the candidate genes? - (reply: 2)
  292. Delta Delta Ct method for multiple reference genes? - (reply: 1)
  293. Cloning a foreign gene into E-coli - (reply: 1)
  294. virus with human gene to mouse cell - Does it work? (reply: 2)
  295. Transfecting a cancer gene - (reply: 2)
  296. housekeeping genes in PCR - (reply: 1)
  297. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
  298. gene expressional analysis - (reply: 1)
  299. Biotinylation sequence: gene orientation?? - (reply: 1)
  300. Can every gene be methylated? - (reply: 1)
  301. Antibody gene cloning - (reply: 3)
  302. Gene not expressed in wild-type and knockout yet differences in microarray - (reply: 15)
  303. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  304. Gene targeting - (reply: 3)
  305. Cheap procedure for gene deletion in E coli? - (reply: 2)
  306. integration of a gene per homologous recombination - (reply: 5)
  307. Ki-67 - no difference bwteen tumor suppressor gene vs ctrl (reply: 3)
  308. relative comparison of many samples and genes in qRT-PCR - (reply: 1)
  309. Reference genes and dilutions in qPCR - (reply: 1)
  310. Molecular identification of ESBL genes blaGES-1, blaVEB-1, blaCTX-M blaOXA-1, bl - International Journal Biological & Life Sciences. 6 (3): 138-142, (reply: 1)
  311. [Software] Drawing arrows as ORFs on genes - (reply: 6)
  312. cre-loxP: how to check if the gene is expressed - (reply: 3)
  313. cre-loxP: how to check if the gene is expressed - (reply: 8)
  314. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
  315. Two genes that are contiguous in the genome... - ...the relationship between them (reply: 7)
  316. clone GENE RT-PCT PRIMERS - cloning genes (reply: 1)
  317. Ghost gene - (reply: 3)
  318. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  319. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
  320. trouble with protein expression of high AT rich gene - (reply: 1)
  321. Cytosolic Housekeeping gene - (reply: 1)
  322. Gene signatures in breast cancer - (reply: 2)
  323. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  324. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
  325. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
  326. How to download the complete list of genes in a chromosome(chromosome 19) - finding complete list of genes in a chomosome in NCBI map viewer (reply: 7)
  327. help with ct of reference gene - (reply: 1)
  328. colony PCR for subcloning gene - (reply: 1)
  329. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  330. siRNA sequence can be used as shRNA? - Stable down regulation of target gene expression. (reply: 1)
  331. how long will it takes to silence target gene after induction of shRNA - (reply: 2)
  332. how to find kappaB binding site on the promoter of a gene - kappaB binding site in gene promoter (reply: 2)
  333. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
  334. Links between genes and risk factors in polygenic diseases - (reply: 2)
  335. Does a viral gene have introns? - (reply: 4)
  336. Blast Nucleotide Issue - No more Gene ID when comparing Bacteria genome (reply: 3)
  337. Gene expression confusion - (reply: 5)
  338. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  339. use of housekeeping gene in RT PCR chIP - (reply: 3)
  340. Gene cross-checking - (reply: 14)
  341. Using Gene Ontology? - (reply: 1)
  342. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
  343. High Gene low protein levels - (reply: 1)
  344. stable clones - correlation between 2 genes in the same family (reply: 1)
  345. how to find TSS of a certain gene - (reply: 1)
  346. How to get a list of all variants (SNPs) within a gene and 5kb immediately 5' - (reply: 3)
  347. About gene orientation - (reply: 2)
  348. very high and inconsistent Cts for the house keeping gene - (reply: 4)
  349. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
  350. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
  351. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
  352. Looking for FASTA format of DNA of a gene amongst various species - (reply: 7)
  353. House keeping gene question? - Thanks for any help! (reply: 1)
  354. Problems with validating primers and low expression genes - (reply: 3)
  355. Deep sequencing to find gene targets of protein? - (reply: 1)
  356. How can multiple cloning sites (MCS) NOT disrupt lacZ gene? - (reply: 2)
  357. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
  358. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
  359. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
  360. Gene expression of methylated gene - (reply: 4)
  361. GAPDH CT lower than target gene CT - (reply: 2)
  362. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
  363. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
  364. mutagenize plasmid-encoded gene - (reply: 4)
  365. Sequencing of gene promoter/terminator, when you only have the AA sequence of th - (reply: 2)
  366. Fusion of reporter genes - (reply: 2)
  367. Cloning from cDNA - the wrong gene?! - (reply: 2)
  368. failure PCR amplification from low GC content gene - (reply: 5)
  369. How many copies of the gene - (reply: 1)
  370. Antibiotic resistance genes in a plasmid - How are they expressed? (reply: 3)
  371. Measuring gene expression across cell lines - (reply: 2)
  372. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
  373. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
  374. How to find gene homologues? - (reply: 1)
  375. microRNAs and mRNAs. Interpreting gene expression profiles - Gene expression profiling and microRNAs (reply: 4)
  376. complete gene for expression? - (reply: 2)
  377. questions on gene therapy in brain - (reply: 1)
  378. 5' RACE for very GC-rich gene - (reply: 2)
  379. How to remove copy of redundant cDNA in a mixture - RT results in a cDNA library with high copy number of few genes (reply: 3)
  380. Large Difference between Ct of 18s rRNA and Target Gene - (reply: 2)
  381. Isoforms of a gene!! - how to find all isoforms of a gene using bioinfo tools?! (reply: 2)
  382. cloning - how to clone a gene having stop codons (reply: 4)
  383. how to intentionally alter gene sequence - (reply: 1)
  384. Relatively unknown gene, need approach suggestions - (reply: 1)
  385. Why is the y-axis labeled 'relative gene expression'? - (reply: 5)
  386. how to express the gene in opposite transcriptional direction - (reply: 3)
  387. How could I express two genes in opposite orientation - (reply: 2)
  388. always run internal control for different genes on the differnet plates? - (reply: 8)
  389. Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable (reply: 1)
  390. help needed: PCR a gene from genomic DNA - (reply: 2)
  391. cloning of cellulose synthase gene - (reply: 1)
  392. Absolute quantification and normalization - Are reference genes necessary? (reply: 1)
  393. Where can you get the sequence of one exon of a gene? - (reply: 3)
  394. Validation of housekeeping gene expression - (reply: 3)
  395. GMO's - introduction of herbicide resistance gene (reply: 3)
  396. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
  397. software for gene ontology - (reply: 2)
  398. Rotor Gene 3000 and Rotor Gene Q - (reply: 1)
  399. designing primers for genes not sequenced yet - (reply: 2)
  400. Expression of a possible toxic gene? - (reply: 5)
  401. get data from gene chip - (reply: 2)
  402. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  403. How to compare different expression levels between genes - (reply: 1)
  404. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  405. miRNA gene UTRs? - (reply: 3)
  406. reference genes for qPCR - (reply: 3)
  407. Q about the housekeeping gene - (reply: 1)
  408. deleting a certain gene from bacterium - (reply: 12)
  409. promoter of a gene - (reply: 2)
  410. searching housekeeping genes - (reply: 4)
  411. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
  412. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
  413. gene copy number calculation - qPCR (reply: 2)
  414. Reference gene qPCR - (reply: 4)
  415. doubt in gene annotation - doubt in submitting gene annotation to NCBI (reply: 1)
  416. relative gene expression with multiple reference genes - data analysis (reply: 1)
  417. How to detect a gene having multiple isoforms using southern/northen techniques - (reply: 1)
  418. What Factors Affect Ct values of a gene? - (reply: 1)
  419. Can linear vectors be transformed and can they express resistance genes? - (reply: 4)
  420. how to test if a given microRNA regulates a gene of interest - microrna target (reply: 7)
  421. how to know a sequene for a specific gene & produce it - (reply: 5)
  422. Gene insertion without desired unique restriction sites - (reply: 3)
  423. Gene cluster drawing software - Anythign to draw genes cluterested together like in an operon (reply: 1)
  424. How to find upstream genomic sequence of a gene? - (reply: 2)
  425. Gene expressed in lenti-packaging cells, but not in lenti-infected cells - (reply: 2)
  426. Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene - (reply: 2)
  427. getting crazy about a gene bank entry - (reply: 2)
  428. Need Help: Bacterial gene deletion- sacB sucrose resistance - (reply: 31)
  429. Problems with gene knockdown - No knockdown with validated shRNAs :( (reply: 6)
  430. problem in gene cloning - (reply: 4)
  431. Trouble with GAPDH housekeeping gene - (reply: 3)
  432. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
  433. HELP: gene with intron expressed in Pichia - (reply: 2)
  434. Promoter/gene cloning question - (reply: 7)
  435. Gene Ontology - Looking for a good software (reply: 3)
  436. Gene screening - (reply: 2)
  437. Gene Expression analysis - (reply: 4)
  438. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  439. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
  440. troubleshooting: gene deletions by method of Wanner and Datsenko - (reply: 2)
  441. yeast transformation - Expression of plant genes in yeast (reply: 1)
  442. Real time house keeping genes change - (reply: 1)
  443. A MAPKKK gene, receptor or not? - (reply: 3)
  444. What genes can be used to validate H3K27me3-ChIP - (reply: 8)
  445. Tool for design gene structure figures - (reply: 5)
  446. how to determine certain gene copy number? - (reply: 3)
  447. expressing data with more than 1 HK gene - (reply: 2)
  448. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
  449. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  450. known gene or area of a chromosome - help (reply: 1)
  451. Amplifying a gene using a degenerate primer - (reply: 4)
  452. Chosing the best housekeeping gene! - (reply: 5)
  453. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
  454. need help on isolation of intron of gene - (reply: 1)
  455. SiRNA- gene silencing in brain Organotypic slice cultures - (reply: 3)
  456. Gene expression in cell line - (reply: 1)
  457. Is H3K4 trimethylation always associated with gene activation? - (reply: 1)
  458. house keeping gene in transgenic mice. - (reply: 1)
  459. Detect gene in stable cell line, - (reply: 4)
  460. selection of internal control gene - (reply: 2)
  461. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
  462. GFP reporter gene - (reply: 3)
  463. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
  464. Bacteria gene expression - (reply: 2)
  465. minimum length for the gene to be amplified in PCR - (reply: 6)
  466. wrong gene - (reply: 13)
  467. gene design - restriction site addition (reply: 2)
  468. Number of genes and number of proteins in humans - (reply: 1)
  469. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
  470. Understanding qPCR and gene expression changes - (reply: 10)
  471. zebrafish gene regulation can I use mammel expression vector? - (reply: 1)
  472. Heterogenous expresion of stably transfected gene - (reply: 4)
  473. assembling problem in genes - assembling problem in genes (reply: 7)
  474. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  475. Promoters and gene expression - (reply: 3)
  476. Automated sequencing - Difficult gene (reply: 1)
  477. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  478. Gene knockin or knockout? transgenic mouse - (reply: 2)
  479. want gene knock out protocol - (reply: 2)
  480. Target gene regulation by transiently transfected PR - (reply: 4)
  481. Mycobacterial housekeeping genes - (reply: 1)
  482. Transfer of gene from one vector to another vector - (reply: 2)
  483. Macroarray platforms - CGH vs Gene Expression (reply: 4)
  484. Houskeeping Gene not steady - (reply: 7)
  485. candidate gene approaches - (reply: 3)
  486. size of gene insert wrong? - (reply: 3)
  487. size of gene insert vs. predicted size - (reply: 1)
  488. Good SiRNA Gene silencing - (reply: 1)
  489. yeast gene deletion - Could I delete an essential gene from haploid cells? (reply: 8)
  490. deleting lacZ gene from control vector - (reply: 5)
  491. methylation by MSSS1(NEB )for reporter gene analysis - (reply: 2)
  492. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  493. RNAi in bacteria - Why no finding for gene silencing in bacteria? (reply: 1)
  494. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)