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Gene Related Discussions
  1. How to get pure protein of a gene - (reply: 1)
  2. How to get pure protein of a gene - (reply: 3)
  3. How to insert genes into chromosome of E. coli - (reply: 1)
  4. Homologous Recombination of 2 genes with greatly different lenghts?. - (reply: 2)
  5. Spliced and unspliced form of a gene - (reply: 3)
  6. Gene synthesis - (reply: 2)
  7. cloning of a long gene using cDNA - (reply: 1)
  8. Finding region of interest in a gene - (reply: 2)
  9. Isolation of a Gene of Interest for Subcloning - (reply: 8)
  10. books about bacteria gene knockout - (reply: 9)
  11. Functional relationships between genes that are contiguous in the genome - (reply: 2)
  12. Too low Ct value for reference gene !!! - (reply: 3)
  13. Problem with expressing genes using tac promoter - (reply: 4)
  14. how to chose right subsequent for her2(erbb2)gene ? - (reply: 1)
  15. Gene expression - (reply: 1)
  16. DNA Construction: HIV-1 Tat Gene - (reply: 9)
  17. qPCR normalization of reference gene - (reply: 1)
  18. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
  19. housekeeping gene has not uniform pattern - (reply: 3)
  20. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  21. cloning an AT rich gene - (reply: 3)
  22. Cloning two genes in the same plasmid - (reply: 4)
  23. RNAi problem - Gene is knocked down, but not protein - (reply: 5)
  24. relative qPCR analysis when gene is not expressed at baseline - (reply: 8)
  25. Any free software for drawing genes? - (reply: 8)
  26. Plasmid, gene orientation, insert orientation. - (reply: 1)
  27. good tutorials/protocols/books on gene cloning - (reply: 2)
  28. Determining gene of interest from Cell culture lines - (reply: 2)
  29. compare between 2 or more genes - (reply: 1)
  30. Knocking out non-essential gene - (reply: 2)
  31. Double digest after cloning doesn't give gene - (reply: 6)
  32. How much of plasmid should be cut to see the gene - (reply: 1)
  33. Comparison of gene structure - (reply: 1)
  34. HaCaT reference gene - (reply: 1)
  35. checking whther there is any miRNA in introns of a specific gene. - (reply: 1)
  36. Need help for PCR for AT rich gene - (reply: 3)
  37. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  38. Relative quantification of different genes in single sample - same threshold val - (reply: 1)
  39. How to find the peptide fragment of target gene？ - (reply: 1)
  40. gene expression in E coli with PET21a vector - (reply: 1)
  41. how to find gene sequences online (need both exons and introns) - (reply: 3)
  42. websits for gene exon and intron sequences - (reply: 1)
  43. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
  44. Cloning promoter (overlap with another gene!) - (reply: 3)
  45. how to know introns of a gene - (reply: 3)
  46. methods for gene sequencing - (reply: 4)
  47. expression of dirrefent isoforms f the same gene in different tissues - (reply: 1)
  48. How do I find the promoter of a gene? - (reply: 3)
  49. How to define a promoter region of a gene? - (reply: 1)
  50. Housekeeping gene for COS-1 cells - (reply: 1)
  51. Low Molecular Weight Housekeeping Gene for Loading Control? - (reply: 2)
  52. Identifying which promoters are regulating gene expression - (reply: 2)
  53. Cell lines & gene of interest - (reply: 1)
  54. Software for selection genes related to concrete cell processes - (reply: 1)
  55. a gene expressed in 293 cell lines but not Hela cells - (reply: 1)
  56. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)
  57. How genes are selectively silenced ? - (reply: 1)
  58. real time qRT-PCR and low expression gene - (reply: 2)
  59. Cloning genes into plant vector - (reply: 1)
  60. PCRing mature form of a gene - (reply: 1)
  61. Gene Expression Issues - (reply: 3)
  62. No gene amplification, 18s amplification is fine - (reply: 1)
  63. Cloning large gene - (reply: 3)
  64. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  65. Confirmed gene knockdown not showing on array - (reply: 2)
  66. how to determine fold change of gene expression - (reply: 2)
  67. negative values after GOI - reference gene ?? - (reply: 4)
  68. Gene expression change after mutagenesis-- please help! - (reply: 4)
  69. Two start codons" the TAG and the gene" - (reply: 1)
  70. expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
  71. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
  72. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
  73. Gene on negative strand - two eductional questions. - (reply: 3)
  74. 3'UTR sequence of a gene - (reply: 3)
  75. Enriched gene signatures - (reply: 1)
  76. how does the binding of miRNA at 3'UTR regulates the gene - (reply: 3)
  77. Sigma esiRNA induces target gene expression - (reply: 1)
  78. High resolution melting to gene scanning - (reply: 1)
  79. Is one reference gene ever appropriate? - (reply: 3)
  80. Trouble to UNDERSTAND gene replacement results - (reply: 1)
  81. Comprehensive database for all the known epigenetic genes in human and mouse - (reply: 2)
  82. Restriction Enzyme sites in my genes - (reply: 1)
  83. infection with two retroviruses? - Delivery of two Genes in distinct virus constructs, does it work? (reply: 3)
  84. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  85. qPCR with low expressing genes - (reply: 1)
  86. Reference genes. - (reply: 1)
  87. Reference genes - (reply: 2)
  88. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
  89. Problem cloning wild type E. coli gene... Possible recombination event in DH5alp - (reply: 4)
  90. Gene set similarity searches in public databases - (reply: 4)
  91. Gene copy number analysis - (reply: 1)
  92. reference gene - (reply: 2)
  93. Predicting function of heterozygous vs WT gene? - (reply: 1)
  94. is there a method to set up stable transfectants without drug selection - no drug-resistant gene (reply: 8)
  95. Selection of housekeeping gene in Mycobacterium - (reply: 1)
  96. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
  97. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
  98. finding exons for a gene, having the coding sequence only - (reply: 4)
  99. New to Gene cloning - Plasmid vector preparation (reply: 2)
  100. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  101. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
  102. copy number of gene in genome - (reply: 4)
  103. Cloning of a gene containing 31 exon - (reply: 1)
  104. predict union between microRNAs and genes - (reply: 2)
  105. A gene is not inserted into the Plasmid - (reply: 2)
  106. Reporter gene to test new promotor - (reply: 1)
  107. How to study with the candidate genes? - (reply: 2)
  108. Delta Delta Ct method for multiple reference genes? - (reply: 1)
  109. Cloning a foreign gene into E-coli - (reply: 1)
  110. virus with human gene to mouse cell - Does it work? (reply: 2)
  111. Transfecting a cancer gene - (reply: 2)
  112. housekeeping genes in PCR - (reply: 1)
  113. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
  114. gene expressional analysis - (reply: 1)
  115. Biotinylation sequence: gene orientation?? - (reply: 1)
  116. Can every gene be methylated? - (reply: 1)
  117. Antibody gene cloning - (reply: 3)
  118. Gene not expressed in wild-type and knockout yet differences in microarray - (reply: 14)
  119. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  120. Gene targeting - (reply: 3)
  121. Cheap procedure for gene deletion in E coli? - (reply: 2)
  122. integration of a gene per homologous recombination - (reply: 5)
  123. Ki-67 - no difference bwteen tumor suppressor gene vs ctrl (reply: 3)
  124. relative comparison of many samples and genes in qRT-PCR - (reply: 1)
  125. Reference genes and dilutions in qPCR - (reply: 1)
  126. Molecular identification of ESBL genes blaGES-1, blaVEB-1, blaCTX-M blaOXA-1, bl - International Journal Biological & Life Sciences. 6 (3): 138-142, (reply: 1)
  127. [Software] Drawing arrows as ORFs on genes - (reply: 5)
  128. cre-loxP: how to check if the gene is expressed - (reply: 3)
  129. cre-loxP: how to check if the gene is expressed - (reply: 8)
  130. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
  131. Two genes that are contiguous in the genome... - ...the relationship between them (reply: 7)
  132. clone GENE RT-PCT PRIMERS - cloning genes (reply: 1)
  133. Ghost gene - (reply: 3)
  134. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  135. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
  136. trouble with protein expression of high AT rich gene - (reply: 1)
  137. Cytosolic Housekeeping gene - (reply: 1)
  138. Gene signatures in breast cancer - (reply: 2)
  139. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  140. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
  141. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 4)
  142. How to download the complete list of genes in a chromosome(chromosome 19) - finding complete list of genes in a chomosome in NCBI map viewer (reply: 7)
  143. help with ct of reference gene - (reply: 1)
  144. colony PCR for subcloning gene - (reply: 1)
  145. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  146. siRNA sequence can be used as shRNA? - Stable down regulation of target gene expression. (reply: 1)
  147. how long will it takes to silence target gene after induction of shRNA - (reply: 2)
  148. how to find kappaB binding site on the promoter of a gene - kappaB binding site in gene promoter (reply: 2)
  149. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
  150. Links between genes and risk factors in polygenic diseases - (reply: 2)
  151. Does a viral gene have introns? - (reply: 4)
  152. Blast Nucleotide Issue - No more Gene ID when comparing Bacteria genome (reply: 3)
  153. Gene expression confusion - (reply: 5)
  154. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  155. use of housekeeping gene in RT PCR chIP - (reply: 3)
  156. Gene cross-checking - (reply: 14)
  157. Using Gene Ontology? - (reply: 1)
  158. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
  159. High Gene low protein levels - (reply: 1)
  160. stable clones - correlation between 2 genes in the same family (reply: 1)
  161. how to find TSS of a certain gene - (reply: 1)
  162. How to get a list of all variants (SNPs) within a gene and 5kb immediately 5' - (reply: 3)
  163. About gene orientation - (reply: 2)
  164. very high and inconsistent Cts for the house keeping gene - (reply: 4)
  165. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
  166. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
  167. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
  168. Looking for FASTA format of DNA of a gene amongst various species - (reply: 7)
  169. House keeping gene question? - Thanks for any help! (reply: 1)
  170. Problems with validating primers and low expression genes - (reply: 3)
  171. Deep sequencing to find gene targets of protein? - (reply: 1)
  172. How can multiple cloning sites (MCS) NOT disrupt lacZ gene? - (reply: 2)
  173. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
  174. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
  175. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
  176. Gene expression of methylated gene - (reply: 4)
  177. GAPDH CT lower than target gene CT - (reply: 2)
  178. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
  179. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
  180. mutagenize plasmid-encoded gene - (reply: 4)
  181. Sequencing of gene promoter/terminator, when you only have the AA sequence of th - (reply: 2)
  182. Fusion of reporter genes - (reply: 2)
  183. Cloning from cDNA - the wrong gene?! - (reply: 2)
  184. failure PCR amplification from low GC content gene - (reply: 5)
  185. How many copies of the gene - (reply: 1)
  186. Antibiotic resistance genes in a plasmid - How are they expressed? (reply: 3)
  187. Measuring gene expression across cell lines - (reply: 2)
  188. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
  189. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
  190. How to find gene homologues? - (reply: 1)
  191. microRNAs and mRNAs. Interpreting gene expression profiles - Gene expression profiling and microRNAs (reply: 4)
  192. complete gene for expression? - (reply: 2)
  193. questions on gene therapy in brain - (reply: 1)
  194. 5' RACE for very GC-rich gene - (reply: 2)
  195. How to remove copy of redundant cDNA in a mixture - RT results in a cDNA library with high copy number of few genes (reply: 3)
  196. Large Difference between Ct of 18s rRNA and Target Gene - (reply: 2)
  197. Isoforms of a gene!! - how to find all isoforms of a gene using bioinfo tools?! (reply: 2)
  198. cloning - how to clone a gene having stop codons (reply: 4)
  199. how to intentionally alter gene sequence - (reply: 1)
  200. Relatively unknown gene, need approach suggestions - (reply: 1)
  201. Why is the y-axis labeled 'relative gene expression'? - (reply: 5)
  202. how to express the gene in opposite transcriptional direction - (reply: 3)
  203. How could I express two genes in opposite orientation - (reply: 2)
  204. always run internal control for different genes on the differnet plates? - (reply: 8)
  205. Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable (reply: 1)
  206. help needed: PCR a gene from genomic DNA - (reply: 2)
  207. cloning of cellulose synthase gene - (reply: 1)
  208. Absolute quantification and normalization - Are reference genes necessary? (reply: 1)
  209. Where can you get the sequence of one exon of a gene? - (reply: 3)
  210. Validation of housekeeping gene expression - (reply: 3)
  211. GMO's - introduction of herbicide resistance gene (reply: 3)
  212. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
  213. software for gene ontology - (reply: 2)
  214. Rotor Gene 3000 and Rotor Gene Q - (reply: 1)
  215. designing primers for genes not sequenced yet - (reply: 2)
  216. Expression of a possible toxic gene? - (reply: 5)
  217. get data from gene chip - (reply: 2)
  218. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  219. How to compare different expression levels between genes - (reply: 1)
  220. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  221. miRNA gene UTRs? - (reply: 3)
  222. reference genes for qPCR - (reply: 3)
  223. Q about the housekeeping gene - (reply: 1)
  224. deleting a certain gene from bacterium - (reply: 12)
  225. promoter of a gene - (reply: 2)
  226. searching housekeeping genes - (reply: 4)
  227. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 3)
  228. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
  229. gene copy number calculation - qPCR (reply: 2)
  230. Reference gene qPCR - (reply: 4)
  231. doubt in gene annotation - doubt in submitting gene annotation to NCBI (reply: 1)
  232. relative gene expression with multiple reference genes - data analysis (reply: 1)
  233. How to detect a gene having multiple isoforms using southern/northen techniques - (reply: 1)
  234. What Factors Affect Ct values of a gene? - (reply: 1)
  235. Can linear vectors be transformed and can they express resistance genes? - (reply: 4)
  236. how to test if a given microRNA regulates a gene of interest - microrna target (reply: 7)
  237. how to know a sequene for a specific gene & produce it - (reply: 5)
  238. Gene insertion without desired unique restriction sites - (reply: 3)
  239. Gene cluster drawing software - Anythign to draw genes cluterested together like in an operon (reply: 1)
  240. How to find upstream genomic sequence of a gene? - (reply: 2)
  241. Gene expressed in lenti-packaging cells, but not in lenti-infected cells - (reply: 2)
  242. Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene - (reply: 2)
  243. getting crazy about a gene bank entry - (reply: 2)
  244. Need Help: Bacterial gene deletion- sacB sucrose resistance - (reply: 31)
  245. Problems with gene knockdown - No knockdown with validated shRNAs :( (reply: 6)
  246. problem in gene cloning - (reply: 4)
  247. Trouble with GAPDH housekeeping gene - (reply: 3)
  248. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
  249. HELP: gene with intron expressed in Pichia - (reply: 2)
  250. Promoter/gene cloning question - (reply: 7)
  251. Gene Ontology - Looking for a good software (reply: 3)
  252. Gene screening - (reply: 2)
  253. Gene Expression analysis - (reply: 4)
  254. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  255. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
  256. troubleshooting: gene deletions by method of Wanner and Datsenko - (reply: 2)
  257. yeast transformation - Expression of plant genes in yeast (reply: 1)
  258. Real time house keeping genes change - (reply: 1)
  259. A MAPKKK gene, receptor or not? - (reply: 3)
  260. What genes can be used to validate H3K27me3-ChIP - (reply: 8)
  261. Tool for design gene structure figures - (reply: 5)
  262. how to determine certain gene copy number? - (reply: 3)
  263. expressing data with more than 1 HK gene - (reply: 2)
  264. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
  265. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  266. known gene or area of a chromosome - help (reply: 1)
  267. Amplifying a gene using a degenerate primer - (reply: 4)
  268. Chosing the best housekeeping gene! - (reply: 5)
  269. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
  270. need help on isolation of intron of gene - (reply: 1)
  271. SiRNA- gene silencing in brain Organotypic slice cultures - (reply: 3)
  272. Gene expression in cell line - (reply: 1)
  273. Is H3K4 trimethylation always associated with gene activation? - (reply: 1)
  274. house keeping gene in transgenic mice. - (reply: 1)
  275. Detect gene in stable cell line, - (reply: 4)
  276. selection of internal control gene - (reply: 2)
  277. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
  278. GFP reporter gene - (reply: 3)
  279. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
  280. Bacteria gene expression - (reply: 2)
  281. minimum length for the gene to be amplified in PCR - (reply: 6)
  282. wrong gene - (reply: 13)
  283. gene design - restriction site addition (reply: 2)
  284. Number of genes and number of proteins in humans - (reply: 1)
  285. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
  286. Understanding qPCR and gene expression changes - (reply: 10)
  287. zebrafish gene regulation can I use mammel expression vector? - (reply: 1)
  288. Heterogenous expresion of stably transfected gene - (reply: 4)
  289. assembling problem in genes - assembling problem in genes (reply: 7)
  290. Program for mapping primers to gene - diagram maker, visualize - (reply: 3)
  291. Promoters and gene expression - (reply: 3)
  292. Automated sequencing - Difficult gene (reply: 1)
  293. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  294. Gene knockin or knockout? transgenic mouse - (reply: 2)
  295. want gene knock out protocol - (reply: 2)
  296. Target gene regulation by transiently transfected PR - (reply: 4)
  297. Mycobacterial housekeeping genes - (reply: 1)
  298. Transfer of gene from one vector to another vector - (reply: 2)
  299. Macroarray platforms - CGH vs Gene Expression (reply: 4)
  300. Houskeeping Gene not steady - (reply: 7)
  301. candidate gene approaches - (reply: 3)
  302. size of gene insert wrong? - (reply: 3)
  303. size of gene insert vs. predicted size - (reply: 1)
  304. Good SiRNA Gene silencing - (reply: 1)
  305. yeast gene deletion - Could I delete an essential gene from haploid cells? (reply: 8)
  306. deleting lacZ gene from control vector - (reply: 5)
  307. methylation by MSSS1(NEB )for reporter gene analysis - (reply: 2)
  308. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  309. RNAi in bacteria - Why no finding for gene silencing in bacteria? (reply: 1)
  310. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)