Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Gene Related Discussions
  1. Housekeeper Genes and Real-Time PCR - (reply: 1)
  2. CRISPR-Cas9 / insert a gene in cell line genome - (reply: 1)
  3. PCR positive control of antibiotics resistance gene - (reply: 1)
  4. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
  5. gene expression in cultured cell lines - (reply: 1)
  6. Map Viewer for several genes - (reply: 1)
  7. Why mutations in different genes cause the same disease across different familie - (reply: 3)
  8. Target gene cloning - (reply: 4)
  9. What are the different methods to inhibit a gene in an organism? - (reply: 1)
  10. Enhancer Identification for Specific Genes - (reply: 3)
  11. What gene to insert in my anti-cancer virus? - (reply: 2)
  12. Gene expression heat map ? - (reply: 1)
  13. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
  14. Cloned gene in pET system not being expressed - (reply: 10)
  15. Question regarding protein conservation among similar genes - (reply: 2)
  16. Problem with subcloning genescript synthesized genes - (reply: 2)
  17. Housekeeping genes - Cq values - (reply: 4)
  18. Can siRNA be used to prevent upregulation in gene expression? - (reply: 3)
  19. Jurkat cell - normal receptor/gene expressivity - (reply: 1)
  20. Using Gene Ontology - (reply: 2)
  21. Inactivating effects on immune cells caused by mutation in the gene encoding the - (reply: 2)
  22. How to identify gene based on molecular weight? - (reply: 4)
  23. Why is the "gene" the smallest unit of heredity? - (reply: 1)
  24. Help Finding Genes In Other Species (Entrez and SPLIGN) - (reply: 1)
  25. qPCR with different genes - (reply: 5)
  26. How to determine gene knock-out from Agarose gel bands - (reply: 1)
  27. Cloning of an unknown gene - (reply: 3)
  28. Cloning of an unknown gene - (reply: 3)
  29. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
  30. How to select promoter for resistance gene in linear/naked DNA "knockout" - (reply: 2)
  31. Cross-species genes on the chromosomes - (reply: 4)
  32. Sequence for nucleotide 845 in HFE gene - (reply: 3)
  33. low copy number housekeeping genes - (reply: 2)
  34. Reference Gene/HKG- every plate - (reply: 2)
  35. qPCR standard for lipase genes - (reply: 2)
  36. Campylobacter jejuni qPCR targeting VS1 gene - (reply: 2)
  37. Gene expression and transcription factors - (reply: 1)
  38. When is it suitable for using NIH3T3 cells to validate cancer genes? - (reply: 1)
  39. How do i validate a cancer gene? - (reply: 3)
  40. problem in sequencing of gene - (reply: 3)
  41. TaqMan gene expression assays - (reply: 1)
  42. primer design for a gene - (reply: 3)
  43. Problems regarding amplification of my gene - (reply: 3)
  44. oncolytic viruses: do they cause oncolytic activity due to excessive gene expres - (reply: 3)
  45. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
  46. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
  47. need some advise about PCR of PKD1-human gene - (reply: 7)
  48. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
  49. Amplification in HK gene but not for target gene - (reply: 3)
  50. orthologs of genes - (reply: 1)
  51. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
  52. Finding out the regulating transcription factors of a list of genes ? - (reply: 1)
  53. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
  54. how can i find whole exons and introns for a special gene - (reply: 8)
  55. KIR gene promoters for MSP primers design - (reply: 2)
  56. What is the RNA:Protein ratio for ONE gene over different RNA levels? - (reply: 1)
  57. Gene knockout results in increasing mouse spleen weight and reducing % eosinophi - (reply: 1)
  58. chk effect of cell line genes on normal dna - (reply: 1)
  59. Ct value of housekeeping gene - (reply: 4)
  60. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
  61. Gene silencing and miRNA isolation - (reply: 1)
  62. Reference gene(s) selection and validaton for qPCR - (reply: 5)
  63. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
  64. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  65. If MCF-7 cell line can expresses Her-2 gene? - (reply: 5)
  66. Gene cloning with unknown sequence - (reply: 6)
  67. Reference genes in miRNAs - (reply: 3)
  68. mRNA copies of each gene under different promoters - (reply: 1)
  69. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
  70. simultaneous knock down of two genes in a single cell line - (reply: 1)
  71. Can we add a restriction site to a gene? - (reply: 1)
  72. Mouse unique genes vs human unique ones - (reply: 3)
  73. Need no RT control for all genes? - (reply: 1)
  74. Different annealing temp between HKG and target gene - (reply: 2)
  75. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  76. How to determine the size of gene to amplify? - (reply: 1)
  77. AZA treatment induces the genes but bisulfite sequencing does not reveal de-meth - (reply: 11)
  78. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  79. How to qualitatively measure DNA methylation of gene promoter - (reply: 1)
  80. Can multiple genes follow only one promoter? - (reply: 1)
  81. does DNA methylation outside promoter region affect gene Transcription/expressio - (reply: 1)
  82. BLAST sequence analysis and gene identification - (reply: 2)
  83. Determining CpG methylation and effect on gene expression - (reply: 1)
  84. i couldn't get the band of target gene after ligation - (reply: 9)
  85. suitable single-copy gene target for fungal qPCR - (reply: 4)
  86. Suppression of a gene by Antisense strategy - (reply: 3)
  87. qPCR of Intronless gene - (reply: 4)
  88. High Ct values in ref gene as well as target gene - (reply: 3)
  89. qPCR reference gene testing help - (reply: 4)
  90. Fanconi anaemia, how was the FANCA gene identified? - (reply: 1)
  91. Step-by-step protocol for newbie in gene expression analysis - (reply: 3)
  92. what is gene codon optimisation. - (reply: 5)
  93. how many copies of the gene are in the genome - (reply: 1)
  94. PCR GAPDH gene - (reply: 1)
  95. Calibrator sample has zero expression of target gene. - (reply: 1)
  96. Functional analysis of microarray gene lists - (reply: 4)
  97. I don't find housekeeping genes: what should I do? - (reply: 6)
  98. Do genes always need to be cloned in the "right direction"? - (reply: 3)
  99. Gene sequence for Real time PCR - (reply: 2)
  100. What is the advantage of knockout over gene therapy in mice model in order to st - (reply: 1)
  101. confirmation of real gene status - (reply: 5)
  102. Finding functions for genes in long gene lists - (reply: 2)
  103. defining ratio of alternative spliced gene with qPCR - (reply: 14)
  104. PCR gene specific amplification problem - (reply: 3)
  105. Reference gene for normalisation - for different growth rates - (reply: 3)
  106. Sorting tools??: Oncogenes/tumor suppressor genes - (reply: 3)
  107. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
  108. Cloning synthesised genes - (reply: 1)
  109. Low Transformation Efficiency with a Specific Gene - (reply: 4)
  110. Gene of interest targeting micrornas - (reply: 6)
  111. gene cloning, where to start? - (reply: 1)
  112. Artificial RNA as normalizer (HK gene) - (reply: 1)
  113. GHR gene location of water buffalo? - (reply: 2)
  114. Insoluble Protein of Interest Resulted from Mammalian Gene Expression - Can Any - (reply: 3)
  115. Gene expression profiling with small cell number - (reply: 1)
  116. Gene expression assay with small cell number - (reply: 3)
  117. BLAST is giving me a result totally unrelated to the gene I expect - (reply: 3)
  118. Two genes, same MCS - (reply: 1)
  119. gene cloning - (reply: 1)
  120. Truncated gene Cloning - (reply: 1)
  121. Over expression of genes and their effect on Apoptosis or mitosis - (reply: 1)
  122. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
  123. His tag introduction in to gene and primer design - (reply: 2)
  124. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
  125. reference gene for qPCR in E.coli - (reply: 2)
  126. Cloning at 6 kb gene - (reply: 3)
  127. Problem amplifying viral gene - (reply: 5)
  128. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
  129. finding gene sequence to delete - (reply: 4)
  130. Designing an experiement to identify mutations in particular genes - (reply: 5)
  131. heterologous gene integration into e.coli genome - (reply: 1)
  132. Finding promoters and genes - (reply: 2)
  133. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
  134. Validation of Up-regulated genes - (reply: 1)
  135. how to find out the DNA seuence of a human gene from online database? - (reply: 1)
  136. Gene expression database - (reply: 1)
  137. How base pairs upstream of gene to include for the promoter? - (reply: 3)
  138. PCR-screening of T-DNA inserted in Arabidopsis gene - (reply: 1)
  139. Change in Superscript lot influences transcript level of one gene but not others - (reply: 2)
  140. Gene knockdown expression levels shRNAmirs - (reply: 2)
  141. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 3)
  142. New gene discovery - (reply: 1)
  143. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
  144. converting protein names protein ids and enterz gene ids - (reply: 1)
  145. Transfection of a non human gene into human cell line - (reply: 1)
  146. looking for a difficult gene - (reply: 1)
  147. Normalization to Standard Gene: what does this mean - (reply: 8)
  148. Tagging a gene with HA and FLAG using PCR - (reply: 1)
  149. Directions of genes expression illustrations - (reply: 5)
  150. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  151. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
  152. Which one is better as reference gene base on these data - (reply: 3)
  153. Restriction enzymes for a gene - (reply: 2)
  154. Better option, mutagenesis or gene synthesis? - (reply: 4)
  155. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  156. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
  157. qPCR: CT value of reference gene - (reply: 3)
  158. cloning a gene in to a plasmid - (reply: 5)
  159. Which cells express which genes? - (reply: 1)
  160. Looking for tool that searches a list of genes for common MiRs - (reply: 1)
  161. How to find promoter sequence of a gene - (reply: 1)
  162. Heterologous gene expression in yeast - a bioinformatician needs your help cloni - (reply: 1)
  163. geNorm alternative for qPCR housekeeping genes - (reply: 1)
  164. Pcr product size determination for a fusion gene - (reply: 8)
  165. How to download whole Genome of Bacteria with Individual genes in FASTA format a - (reply: 3)
  166. validation of microRNA target gene - (reply: 6)
  167. Textbook Canon: Expressing Any Genes in Bacteria - (reply: 21)
  168. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
  169. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
  170. Alternative to gene silencing for functional assay?? much help needed :) - (reply: 1)
  171. Taqman assay for detecting expression of genes carrying specific SNPs - (reply: 2)
  172. Trick for making diagram of gene for paper figure? - (reply: 4)
  173. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
  174. Best way to genotpy a mutant gene with a 1bp difference. - (reply: 3)
  175. A good vector to make gene library? - (reply: 5)
  176. SNP genotyping of whole gene - (reply: 4)
  177. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  178. Receptor and gene interaction - (reply: 2)
  179. Transformation of an inducible GFP gene into Aspergillus niger - (reply: 2)
  180. Gene expression - (reply: 5)
  181. Help with CGL gene cloning problems - (reply: 1)
  182. sequencing result doesn't match my gene.... - (reply: 5)
  183. How to get pure protein of a gene - (reply: 1)
  184. How to get pure protein of a gene - (reply: 3)
  185. How to insert genes into chromosome of E. coli - (reply: 1)
  186. Homologous Recombination of 2 genes with greatly different lenghts?. - (reply: 2)
  187. Spliced and unspliced form of a gene - (reply: 3)
  188. Gene synthesis - (reply: 2)
  189. cloning of a long gene using cDNA - (reply: 1)
  190. Finding region of interest in a gene - (reply: 2)
  191. Isolation of a Gene of Interest for Subcloning - (reply: 8)
  192. books for bacteria gene knockout - (reply: 1)
  193. books about bacteria gene knockout - (reply: 9)
  194. Functional relationships between genes that are contiguous in the genome - (reply: 2)
  195. Too low Ct value for reference gene !!! - (reply: 3)
  196. Problem with expressing genes using tac promoter - (reply: 4)
  197. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
  198. Gene expression - (reply: 1)
  199. DNA Construction: HIV-1 Tat Gene - (reply: 9)
  200. qPCR normalization of reference gene - (reply: 1)
  201. inquiry about the methods for gene knockout in bacteria - (reply: 1)
  202. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
  203. housekeeping gene has not uniform pattern - (reply: 3)
  204. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  205. cloning an AT rich gene - (reply: 3)
  206. Cloning two genes in the same plasmid - (reply: 4)
  207. RNAi problem - Gene is knocked down, but not protein - (reply: 9)
  208. relative qPCR analysis when gene is not expressed at baseline - (reply: 8)
  209. Any free software for drawing genes? - (reply: 9)
  210. Plasmid, gene orientation, insert orientation. - (reply: 1)
  211. good tutorials/protocols/books on gene cloning - (reply: 2)
  212. Determining gene of interest from Cell culture lines - (reply: 2)
  213. compare between 2 or more genes - (reply: 1)
  214. Knocking out non-essential gene - (reply: 2)
  215. Double digest after cloning doesn't give gene - (reply: 6)
  216. How much of plasmid should be cut to see the gene - (reply: 1)
  217. Comparison of gene structure - (reply: 1)
  218. HaCaT reference gene - (reply: 1)
  219. checking whther there is any miRNA in introns of a specific gene. - (reply: 1)
  220. Need help for PCR for AT rich gene - (reply: 3)
  221. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  222. Relative quantification of different genes in single sample - same threshold val - (reply: 1)
  223. How to find the peptide fragment of target gene? - (reply: 1)
  224. gene expression in E coli with PET21a vector - (reply: 1)
  225. how to find gene sequences online (need both exons and introns) - (reply: 3)
  226. websits for gene exon and intron sequences - (reply: 1)
  227. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
  228. Cloning promoter (overlap with another gene!) - (reply: 3)
  229. how to know introns of a gene - (reply: 3)
  230. methods for gene sequencing - (reply: 4)
  231. expression of dirrefent isoforms f the same gene in different tissues - (reply: 1)
  232. How do I find the promoter of a gene? - (reply: 3)
  233. How to define a promoter region of a gene? - (reply: 1)
  234. Housekeeping gene for COS-1 cells - (reply: 1)
  235. Low Molecular Weight Housekeeping Gene for Loading Control? - (reply: 2)
  236. Identifying which promoters are regulating gene expression - (reply: 2)
  237. Cell lines & gene of interest - (reply: 1)
  238. Software for selection genes related to concrete cell processes - (reply: 1)
  239. a gene expressed in 293 cell lines but not Hela cells - (reply: 1)
  240. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)
  241. How genes are selectively silenced ? - (reply: 1)
  242. real time qRT-PCR and low expression gene - (reply: 2)
  243. Cloning genes into plant vector - (reply: 1)
  244. PCRing mature form of a gene - (reply: 1)
  245. Gene Expression Issues - (reply: 3)
  246. No gene amplification, 18s amplification is fine - (reply: 1)
  247. Cloning large gene - (reply: 3)
  248. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  249. Confirmed gene knockdown not showing on array - (reply: 2)
  250. how to determine fold change of gene expression - (reply: 2)
  251. negative values after GOI - reference gene ?? - (reply: 4)
  252. Gene expression change after mutagenesis-- please help! - (reply: 4)
  253. Two start codons" the TAG and the gene" - (reply: 1)
  254. expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
  255. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
  256. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
  257. Gene on negative strand - two eductional questions. - (reply: 3)
  258. 3'UTR sequence of a gene - (reply: 3)
  259. Enriched gene signatures - (reply: 1)
  260. how does the binding of miRNA at 3'UTR regulates the gene - (reply: 3)
  261. Sigma esiRNA induces target gene expression - (reply: 1)
  262. High resolution melting to gene scanning - (reply: 1)
  263. Is one reference gene ever appropriate? - (reply: 3)
  264. Trouble to UNDERSTAND gene replacement results - (reply: 1)
  265. Comprehensive database for all the known epigenetic genes in human and mouse - (reply: 2)
  266. Restriction Enzyme sites in my genes - (reply: 1)
  267. infection with two retroviruses? - Delivery of two Genes in distinct virus constructs, does it work? (reply: 3)
  268. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  269. qPCR with low expressing genes - (reply: 1)
  270. Reference genes. - (reply: 1)
  271. Reference genes - (reply: 2)
  272. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
  273. Problem cloning wild type E. coli gene... Possible recombination event in DH5alp - (reply: 4)
  274. Gene set similarity searches in public databases - (reply: 4)
  275. Gene copy number analysis - (reply: 1)
  276. reference gene - (reply: 2)
  277. Predicting function of heterozygous vs WT gene? - (reply: 1)
  278. is there a method to set up stable transfectants without drug selection - no drug-resistant gene (reply: 8)
  279. Selection of housekeeping gene in Mycobacterium - (reply: 1)
  280. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
  281. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
  282. finding exons for a gene, having the coding sequence only - (reply: 4)
  283. New to Gene cloning - Plasmid vector preparation (reply: 2)
  284. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  285. Regression analysis between two genes - (reply: 1)
  286. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
  287. copy number of gene in genome - (reply: 4)
  288. Cloning of a gene containing 31 exon - (reply: 1)
  289. predict union between microRNAs and genes - (reply: 2)
  290. A gene is not inserted into the Plasmid - (reply: 2)
  291. Reporter gene to test new promotor - (reply: 1)
  292. How to study with the candidate genes? - (reply: 2)
  293. Delta Delta Ct method for multiple reference genes? - (reply: 1)
  294. Cloning a foreign gene into E-coli - (reply: 1)
  295. virus with human gene to mouse cell - Does it work? (reply: 2)
  296. Transfecting a cancer gene - (reply: 2)
  297. housekeeping genes in PCR - (reply: 1)
  298. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
  299. gene expressional analysis - (reply: 1)
  300. Biotinylation sequence: gene orientation?? - (reply: 1)
  301. Can every gene be methylated? - (reply: 1)
  302. Antibody gene cloning - (reply: 3)
  303. Gene not expressed in wild-type and knockout yet differences in microarray - (reply: 15)
  304. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  305. Gene targeting - (reply: 3)
  306. Cheap procedure for gene deletion in E coli? - (reply: 2)
  307. integration of a gene per homologous recombination - (reply: 5)
  308. Ki-67 - no difference bwteen tumor suppressor gene vs ctrl (reply: 3)
  309. relative comparison of many samples and genes in qRT-PCR - (reply: 1)
  310. Reference genes and dilutions in qPCR - (reply: 1)
  311. Molecular identification of ESBL genes blaGES-1, blaVEB-1, blaCTX-M blaOXA-1, bl - International Journal Biological & Life Sciences. 6 (3): 138-142, (reply: 1)
  312. [Software] Drawing arrows as ORFs on genes - (reply: 6)
  313. cre-loxP: how to check if the gene is expressed - (reply: 3)
  314. cre-loxP: how to check if the gene is expressed - (reply: 8)
  315. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
  316. Two genes that are contiguous in the genome... - ...the relationship between them (reply: 7)
  317. clone GENE RT-PCT PRIMERS - cloning genes (reply: 1)
  318. Ghost gene - (reply: 3)
  319. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  320. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
  321. trouble with protein expression of high AT rich gene - (reply: 1)
  322. Cytosolic Housekeeping gene - (reply: 1)
  323. Gene signatures in breast cancer - (reply: 2)
  324. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  325. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
  326. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
  327. How to download the complete list of genes in a chromosome(chromosome 19) - finding complete list of genes in a chomosome in NCBI map viewer (reply: 7)
  328. help with ct of reference gene - (reply: 1)
  329. colony PCR for subcloning gene - (reply: 1)
  330. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  331. siRNA sequence can be used as shRNA? - Stable down regulation of target gene expression. (reply: 1)
  332. how long will it takes to silence target gene after induction of shRNA - (reply: 2)
  333. how to find kappaB binding site on the promoter of a gene - kappaB binding site in gene promoter (reply: 2)
  334. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
  335. Links between genes and risk factors in polygenic diseases - (reply: 2)
  336. Does a viral gene have introns? - (reply: 4)
  337. Blast Nucleotide Issue - No more Gene ID when comparing Bacteria genome (reply: 3)
  338. Gene expression confusion - (reply: 5)
  339. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  340. use of housekeeping gene in RT PCR chIP - (reply: 3)
  341. Gene cross-checking - (reply: 14)
  342. Using Gene Ontology? - (reply: 1)
  343. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
  344. High Gene low protein levels - (reply: 1)
  345. stable clones - correlation between 2 genes in the same family (reply: 1)
  346. how to find TSS of a certain gene - (reply: 1)
  347. How to get a list of all variants (SNPs) within a gene and 5kb immediately 5' - (reply: 3)
  348. About gene orientation - (reply: 2)
  349. very high and inconsistent Cts for the house keeping gene - (reply: 4)
  350. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
  351. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
  352. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
  353. Looking for FASTA format of DNA of a gene amongst various species - (reply: 7)
  354. House keeping gene question? - Thanks for any help! (reply: 1)
  355. Problems with validating primers and low expression genes - (reply: 3)
  356. Deep sequencing to find gene targets of protein? - (reply: 1)
  357. How can multiple cloning sites (MCS) NOT disrupt lacZ gene? - (reply: 2)
  358. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
  359. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
  360. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
  361. Gene expression of methylated gene - (reply: 4)
  362. GAPDH CT lower than target gene CT - (reply: 2)
  363. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
  364. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
  365. mutagenize plasmid-encoded gene - (reply: 4)
  366. Sequencing of gene promoter/terminator, when you only have the AA sequence of th - (reply: 2)
  367. Fusion of reporter genes - (reply: 2)
  368. Cloning from cDNA - the wrong gene?! - (reply: 2)
  369. failure PCR amplification from low GC content gene - (reply: 5)
  370. How many copies of the gene - (reply: 1)
  371. Antibiotic resistance genes in a plasmid - How are they expressed? (reply: 3)
  372. Measuring gene expression across cell lines - (reply: 2)
  373. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
  374. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
  375. How to find gene homologues? - (reply: 1)
  376. microRNAs and mRNAs. Interpreting gene expression profiles - Gene expression profiling and microRNAs (reply: 4)
  377. complete gene for expression? - (reply: 2)
  378. questions on gene therapy in brain - (reply: 1)
  379. 5' RACE for very GC-rich gene - (reply: 2)
  380. How to remove copy of redundant cDNA in a mixture - RT results in a cDNA library with high copy number of few genes (reply: 3)
  381. Large Difference between Ct of 18s rRNA and Target Gene - (reply: 2)
  382. Isoforms of a gene!! - how to find all isoforms of a gene using bioinfo tools?! (reply: 2)
  383. cloning - how to clone a gene having stop codons (reply: 4)
  384. how to intentionally alter gene sequence - (reply: 1)
  385. Relatively unknown gene, need approach suggestions - (reply: 1)
  386. Why is the y-axis labeled 'relative gene expression'? - (reply: 5)
  387. how to express the gene in opposite transcriptional direction - (reply: 3)
  388. How could I express two genes in opposite orientation - (reply: 2)
  389. always run internal control for different genes on the differnet plates? - (reply: 8)
  390. Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable (reply: 1)
  391. help needed: PCR a gene from genomic DNA - (reply: 2)
  392. cloning of cellulose synthase gene - (reply: 1)
  393. Absolute quantification and normalization - Are reference genes necessary? (reply: 1)
  394. Where can you get the sequence of one exon of a gene? - (reply: 3)
  395. Validation of housekeeping gene expression - (reply: 3)
  396. GMO's - introduction of herbicide resistance gene (reply: 3)
  397. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
  398. software for gene ontology - (reply: 2)
  399. Rotor Gene 3000 and Rotor Gene Q - (reply: 1)
  400. designing primers for genes not sequenced yet - (reply: 2)
  401. Expression of a possible toxic gene? - (reply: 5)
  402. get data from gene chip - (reply: 2)
  403. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  404. How to compare different expression levels between genes - (reply: 1)
  405. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  406. miRNA gene UTRs? - (reply: 3)
  407. reference genes for qPCR - (reply: 3)
  408. Q about the housekeeping gene - (reply: 1)
  409. deleting a certain gene from bacterium - (reply: 12)
  410. promoter of a gene - (reply: 2)
  411. searching housekeeping genes - (reply: 4)
  412. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
  413. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
  414. gene copy number calculation - qPCR (reply: 2)
  415. Reference gene qPCR - (reply: 4)
  416. doubt in gene annotation - doubt in submitting gene annotation to NCBI (reply: 1)
  417. relative gene expression with multiple reference genes - data analysis (reply: 1)
  418. How to detect a gene having multiple isoforms using southern/northen techniques - (reply: 1)
  419. What Factors Affect Ct values of a gene? - (reply: 1)
  420. Can linear vectors be transformed and can they express resistance genes? - (reply: 4)
  421. how to test if a given microRNA regulates a gene of interest - microrna target (reply: 7)
  422. how to know a sequene for a specific gene & produce it - (reply: 5)
  423. Gene insertion without desired unique restriction sites - (reply: 3)
  424. Gene cluster drawing software - Anythign to draw genes cluterested together like in an operon (reply: 1)
  425. How to find upstream genomic sequence of a gene? - (reply: 2)
  426. Gene expressed in lenti-packaging cells, but not in lenti-infected cells - (reply: 2)
  427. Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene - (reply: 2)
  428. getting crazy about a gene bank entry - (reply: 2)
  429. Need Help: Bacterial gene deletion- sacB sucrose resistance - (reply: 31)
  430. Problems with gene knockdown - No knockdown with validated shRNAs :( (reply: 6)
  431. problem in gene cloning - (reply: 4)
  432. Trouble with GAPDH housekeeping gene - (reply: 3)
  433. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
  434. HELP: gene with intron expressed in Pichia - (reply: 2)
  435. Promoter/gene cloning question - (reply: 7)
  436. Gene Ontology - Looking for a good software (reply: 3)
  437. Gene screening - (reply: 2)
  438. Gene Expression analysis - (reply: 4)
  439. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  440. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
  441. troubleshooting: gene deletions by method of Wanner and Datsenko - (reply: 2)
  442. yeast transformation - Expression of plant genes in yeast (reply: 1)
  443. Real time house keeping genes change - (reply: 1)
  444. A MAPKKK gene, receptor or not? - (reply: 3)
  445. What genes can be used to validate H3K27me3-ChIP - (reply: 8)
  446. Tool for design gene structure figures - (reply: 5)
  447. how to determine certain gene copy number? - (reply: 3)
  448. expressing data with more than 1 HK gene - (reply: 2)
  449. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
  450. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  451. known gene or area of a chromosome - help (reply: 1)
  452. Amplifying a gene using a degenerate primer - (reply: 4)
  453. Chosing the best housekeeping gene! - (reply: 5)
  454. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
  455. need help on isolation of intron of gene - (reply: 1)
  456. SiRNA- gene silencing in brain Organotypic slice cultures - (reply: 3)
  457. Gene expression in cell line - (reply: 1)
  458. Is H3K4 trimethylation always associated with gene activation? - (reply: 1)
  459. house keeping gene in transgenic mice. - (reply: 1)
  460. Detect gene in stable cell line, - (reply: 4)
  461. selection of internal control gene - (reply: 2)
  462. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
  463. GFP reporter gene - (reply: 3)
  464. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
  465. Bacteria gene expression - (reply: 2)
  466. minimum length for the gene to be amplified in PCR - (reply: 6)
  467. wrong gene - (reply: 13)
  468. gene design - restriction site addition (reply: 2)
  469. Number of genes and number of proteins in humans - (reply: 1)
  470. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
  471. Understanding qPCR and gene expression changes - (reply: 10)
  472. zebrafish gene regulation can I use mammel expression vector? - (reply: 1)
  473. Heterogenous expresion of stably transfected gene - (reply: 4)
  474. assembling problem in genes - assembling problem in genes (reply: 7)
  475. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  476. Promoters and gene expression - (reply: 3)
  477. Automated sequencing - Difficult gene (reply: 1)
  478. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  479. Gene knockin or knockout? transgenic mouse - (reply: 2)
  480. want gene knock out protocol - (reply: 2)
  481. Target gene regulation by transiently transfected PR - (reply: 4)
  482. Mycobacterial housekeeping genes - (reply: 1)
  483. Transfer of gene from one vector to another vector - (reply: 2)
  484. Macroarray platforms - CGH vs Gene Expression (reply: 4)
  485. Houskeeping Gene not steady - (reply: 7)
  486. candidate gene approaches - (reply: 3)
  487. size of gene insert wrong? - (reply: 3)
  488. size of gene insert vs. predicted size - (reply: 1)
  489. Good SiRNA Gene silencing - (reply: 1)
  490. yeast gene deletion - Could I delete an essential gene from haploid cells? (reply: 8)
  491. deleting lacZ gene from control vector - (reply: 5)
  492. methylation by MSSS1(NEB )for reporter gene analysis - (reply: 2)
  493. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  494. RNAi in bacteria - Why no finding for gene silencing in bacteria? (reply: 1)
  495. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)