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Gene Related Discussions
  1. Target gene cloning - (reply: 4)
  2. Enhancer Identification for Specific Genes - (reply: 3)
  3. What gene to insert in my anti-cancer virus? - (reply: 2)
  4. Gene expression heat map ? - (reply: 1)
  5. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
  6. Cloned gene in pET system not being expressed - (reply: 10)
  7. Question regarding protein conservation among similar genes - (reply: 2)
  8. Problem with subcloning genescript synthesized genes - (reply: 1)
  9. Housekeeping genes - Cq values - (reply: 4)
  10. Can siRNA be used to prevent upregulation in gene expression? - (reply: 3)
  11. Jurkat cell - normal receptor/gene expressivity - (reply: 1)
  12. Using Gene Ontology - (reply: 2)
  13. Inactivating effects on immune cells caused by mutation in the gene encoding the - (reply: 2)
  14. How to identify gene based on molecular weight? - (reply: 4)
  15. Why is the "gene" the smallest unit of heredity? - (reply: 1)
  16. Help Finding Genes In Other Species (Entrez and SPLIGN) - (reply: 1)
  17. qPCR with different genes - (reply: 5)
  18. How to determine gene knock-out from Agarose gel bands - (reply: 1)
  19. Cloning of an unknown gene - (reply: 3)
  20. Cloning of an unknown gene - (reply: 3)
  21. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
  22. How to select promoter for resistance gene in linear/naked DNA "knockout" - (reply: 2)
  23. Cross-species genes on the chromosomes - (reply: 4)
  24. Sequence for nucleotide 845 in HFE gene - (reply: 3)
  25. low copy number housekeeping genes - (reply: 2)
  26. Reference Gene/HKG- every plate - (reply: 2)
  27. qPCR standard for lipase genes - (reply: 2)
  28. Campylobacter jejuni qPCR targeting VS1 gene - (reply: 2)
  29. Gene expression and transcription factors - (reply: 1)
  30. When is it suitable for using NIH3T3 cells to validate cancer genes? - (reply: 1)
  31. How do i validate a cancer gene? - (reply: 3)
  32. problem in sequencing of gene - (reply: 3)
  33. TaqMan gene expression assays - (reply: 1)
  34. primer design for a gene - (reply: 3)
  35. Problems regarding amplification of my gene - (reply: 3)
  36. oncolytic viruses: do they cause oncolytic activity due to excessive gene expres - (reply: 3)
  37. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
  38. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
  39. need some advise about PCR of PKD1-human gene - (reply: 7)
  40. Restriction-free cloning to exchange gene in vector backbone - (reply: 8)
  41. Amplification in HK gene but not for target gene - (reply: 3)
  42. orthologs of genes - (reply: 1)
  43. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
  44. Finding out the regulating transcription factors of a list of genes ? - (reply: 1)
  45. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
  46. how can i find whole exons and introns for a special gene - (reply: 8)
  47. KIR gene promoters for MSP primers design - (reply: 2)
  48. What is the RNA:Protein ratio for ONE gene over different RNA levels? - (reply: 1)
  49. Gene knockout results in increasing mouse spleen weight and reducing % eosinophi - (reply: 1)
  50. chk effect of cell line genes on normal dna - (reply: 1)
  51. Ct value of housekeeping gene - (reply: 4)
  52. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
  53. Gene silencing and miRNA isolation - (reply: 1)
  54. Reference gene(s) selection and validaton for qPCR - (reply: 5)
  55. How to clone a gene whose genome has not been sequenced yet? - (reply: 4)
  56. Cloning two genes in the same plasmid with one promotor - (reply: 4)
  57. If MCF-7 cell line can expresses Her-2 gene? - (reply: 5)
  58. Gene cloning with unknown sequence - (reply: 6)
  59. Reference genes in miRNAs - (reply: 3)
  60. mRNA copies of each gene under different promoters - (reply: 1)
  61. Ideas wanted-How to clone 600bp gene with two 5' 55base overhangs? - (reply: 1)
  62. simultaneous knock down of two genes in a single cell line - (reply: 1)
  63. Can we add a restriction site to a gene? - (reply: 1)
  64. Mouse unique genes vs human unique ones - (reply: 3)
  65. Need no RT control for all genes? - (reply: 1)
  66. Different annealing temp between HKG and target gene - (reply: 2)
  67. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  68. How to determine the size of gene to amplify? - (reply: 1)
  69. AZA treatment induces the genes but bisulfite sequencing does not reveal de-meth - (reply: 11)
  70. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  71. How to qualitatively measure DNA methylation of gene promoter - (reply: 1)
  72. Can multiple genes follow only one promoter? - (reply: 1)
  73. does DNA methylation outside promoter region affect gene Transcription/expressio - (reply: 1)
  74. BLAST sequence analysis and gene identification - (reply: 2)
  75. Determining CpG methylation and effect on gene expression - (reply: 1)
  76. i couldn't get the band of target gene after ligation - (reply: 9)
  77. suitable single-copy gene target for fungal qPCR - (reply: 4)
  78. Suppression of a gene by Antisense strategy - (reply: 3)
  79. qPCR of Intronless gene - (reply: 4)
  80. High Ct values in ref gene as well as target gene - (reply: 3)
  81. qPCR reference gene testing help - (reply: 4)
  82. Fanconi anaemia, how was the FANCA gene identified? - (reply: 1)
  83. Step-by-step protocol for newbie in gene expression analysis - (reply: 3)
  84. what is gene codon optimisation. - (reply: 5)
  85. how many copies of the gene are in the genome - (reply: 1)
  86. PCR GAPDH gene - (reply: 1)
  87. Calibrator sample has zero expression of target gene. - (reply: 1)
  88. Functional analysis of microarray gene lists - (reply: 4)
  89. I don't find housekeeping genes: what should I do? - (reply: 6)
  90. Do genes always need to be cloned in the "right direction"? - (reply: 3)
  91. Gene sequence for Real time PCR - (reply: 2)
  92. What is the advantage of knockout over gene therapy in mice model in order to st - (reply: 1)
  93. confirmation of real gene status - (reply: 5)
  94. Finding functions for genes in long gene lists - (reply: 2)
  95. defining ratio of alternative spliced gene with qPCR - (reply: 14)
  96. PCR gene specific amplification problem - (reply: 3)
  97. Reference gene for normalisation - for different growth rates - (reply: 3)
  98. Sorting tools??: Oncogenes/tumor suppressor genes - (reply: 3)
  99. Puzzling difficulty cutting out one gene sequence for another - (reply: 4)
  100. Cloning synthesised genes - (reply: 1)
  101. Low Transformation Efficiency with a Specific Gene - (reply: 4)
  102. Gene of interest targeting micrornas - (reply: 6)
  103. gene cloning, where to start? - (reply: 1)
  104. Artificial RNA as normalizer (HK gene) - (reply: 1)
  105. GHR gene location of water buffalo? - (reply: 2)
  106. Insoluble Protein of Interest Resulted from Mammalian Gene Expression - Can Any - (reply: 3)
  107. Gene expression profiling with small cell number - (reply: 1)
  108. Gene expression assay with small cell number - (reply: 3)
  109. BLAST is giving me a result totally unrelated to the gene I expect - (reply: 3)
  110. Two genes, same MCS - (reply: 1)
  111. gene cloning - (reply: 1)
  112. Truncated gene Cloning - (reply: 1)
  113. Over expression of genes and their effect on Apoptosis or mitosis - (reply: 1)
  114. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
  115. His tag introduction in to gene and primer design - (reply: 2)
  116. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
  117. reference gene for qPCR in E.coli - (reply: 2)
  118. Cloning at 6 kb gene - (reply: 3)
  119. Problem amplifying viral gene - (reply: 5)
  120. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
  121. finding gene sequence to delete - (reply: 4)
  122. Designing an experiement to identify mutations in particular genes - (reply: 5)
  123. heterologous gene integration into e.coli genome - (reply: 1)
  124. Finding promoters and genes - (reply: 2)
  125. Not RNAi. Has anyone tried gene knock-up? - (reply: 2)
  126. Validation of Up-regulated genes - (reply: 1)
  127. how to find out the DNA seuence of a human gene from online database? - (reply: 1)
  128. Gene expression database - (reply: 1)
  129. How base pairs upstream of gene to include for the promoter? - (reply: 3)
  130. PCR-screening of T-DNA inserted in Arabidopsis gene - (reply: 1)
  131. Change in Superscript lot influences transcript level of one gene but not others - (reply: 2)
  132. Gene knockdown expression levels shRNAmirs - (reply: 2)
  133. After ligation,I found that my colony on amplicilin plate have not insert gene - (reply: 1)
  134. New gene discovery - (reply: 1)
  135. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
  136. converting protein names protein ids and enterz gene ids - (reply: 1)
  137. Transfection of a non human gene into human cell line - (reply: 1)
  138. looking for a difficult gene - (reply: 1)
  139. Normalization to Standard Gene: what does this mean - (reply: 8)
  140. Tagging a gene with HA and FLAG using PCR - (reply: 1)
  141. Directions of genes expression illustrations - (reply: 5)
  142. different orientation or direction of genes in expression Plasmid vector - (reply: 4)
  143. shRNA designed for AB1 gene also knockdown AB2 - (reply: 2)
  144. Which one is better as reference gene base on these data - (reply: 3)
  145. Restriction enzymes for a gene - (reply: 2)
  146. Better option, mutagenesis or gene synthesis? - (reply: 4)
  147. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  148. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
  149. qPCR: CT value of reference gene - (reply: 3)
  150. cloning a gene in to a plasmid - (reply: 5)
  151. Which cells express which genes? - (reply: 1)
  152. Looking for tool that searches a list of genes for common MiRs - (reply: 1)
  153. How to find promoter sequence of a gene - (reply: 1)
  154. Heterologous gene expression in yeast - a bioinformatician needs your help cloni - (reply: 1)
  155. geNorm alternative for qPCR housekeeping genes - (reply: 1)
  156. Pcr product size determination for a fusion gene - (reply: 8)
  157. How to download whole Genome of Bacteria with Individual genes in FASTA format a - (reply: 3)
  158. validation of microRNA target gene - (reply: 6)
  159. Textbook Canon: Expressing Any Genes in Bacteria - (reply: 21)
  160. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
  161. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
  162. Alternative to gene silencing for functional assay?? much help needed :) - (reply: 1)
  163. Taqman assay for detecting expression of genes carrying specific SNPs - (reply: 2)
  164. Trick for making diagram of gene for paper figure? - (reply: 4)
  165. Is the Kozak sequence really needed for cloning to express the gene? - (reply: 3)
  166. Best way to genotpy a mutant gene with a 1bp difference. - (reply: 3)
  167. A good vector to make gene library? - (reply: 5)
  168. SNP genotyping of whole gene - (reply: 4)
  169. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  170. Receptor and gene interaction - (reply: 2)
  171. Transformation of an inducible GFP gene into Aspergillus niger - (reply: 2)
  172. Gene expression - (reply: 5)
  173. Help with CGL gene cloning problems - (reply: 1)
  174. sequencing result doesn't match my gene.... - (reply: 5)
  175. How to get pure protein of a gene - (reply: 1)
  176. How to get pure protein of a gene - (reply: 3)
  177. How to insert genes into chromosome of E. coli - (reply: 1)
  178. Homologous Recombination of 2 genes with greatly different lenghts?. - (reply: 2)
  179. Spliced and unspliced form of a gene - (reply: 3)
  180. Gene synthesis - (reply: 2)
  181. cloning of a long gene using cDNA - (reply: 1)
  182. Finding region of interest in a gene - (reply: 2)
  183. Isolation of a Gene of Interest for Subcloning - (reply: 8)
  184. books for bacteria gene knockout - (reply: 1)
  185. books about bacteria gene knockout - (reply: 9)
  186. Functional relationships between genes that are contiguous in the genome - (reply: 2)
  187. Too low Ct value for reference gene !!! - (reply: 3)
  188. Problem with expressing genes using tac promoter - (reply: 4)
  189. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
  190. Gene expression - (reply: 1)
  191. DNA Construction: HIV-1 Tat Gene - (reply: 9)
  192. qPCR normalization of reference gene - (reply: 1)
  193. inquiry about the methods for gene knockout in bacteria - (reply: 1)
  194. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
  195. housekeeping gene has not uniform pattern - (reply: 3)
  196. Does KanR gene driven by Kanamycin promoter on a plasmid (e.g., pCR4TOPO) confer - (reply: 3)
  197. cloning an AT rich gene - (reply: 3)
  198. Cloning two genes in the same plasmid - (reply: 4)
  199. RNAi problem - Gene is knocked down, but not protein - (reply: 9)
  200. relative qPCR analysis when gene is not expressed at baseline - (reply: 8)
  201. Any free software for drawing genes? - (reply: 9)
  202. Plasmid, gene orientation, insert orientation. - (reply: 1)
  203. good tutorials/protocols/books on gene cloning - (reply: 2)
  204. Determining gene of interest from Cell culture lines - (reply: 2)
  205. compare between 2 or more genes - (reply: 1)
  206. Knocking out non-essential gene - (reply: 2)
  207. Double digest after cloning doesn't give gene - (reply: 6)
  208. How much of plasmid should be cut to see the gene - (reply: 1)
  209. Comparison of gene structure - (reply: 1)
  210. HaCaT reference gene - (reply: 1)
  211. checking whther there is any miRNA in introns of a specific gene. - (reply: 1)
  212. Need help for PCR for AT rich gene - (reply: 3)
  213. How big is big in terms of the gene of interest insert in plasmid? - (reply: 1)
  214. Relative quantification of different genes in single sample - same threshold val - (reply: 1)
  215. How to find the peptide fragment of target gene? - (reply: 1)
  216. gene expression in E coli with PET21a vector - (reply: 1)
  217. how to find gene sequences online (need both exons and introns) - (reply: 3)
  218. websits for gene exon and intron sequences - (reply: 1)
  219. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
  220. Cloning promoter (overlap with another gene!) - (reply: 3)
  221. how to know introns of a gene - (reply: 3)
  222. methods for gene sequencing - (reply: 4)
  223. expression of dirrefent isoforms f the same gene in different tissues - (reply: 1)
  224. How do I find the promoter of a gene? - (reply: 3)
  225. How to define a promoter region of a gene? - (reply: 1)
  226. Housekeeping gene for COS-1 cells - (reply: 1)
  227. Low Molecular Weight Housekeeping Gene for Loading Control? - (reply: 2)
  228. Identifying which promoters are regulating gene expression - (reply: 2)
  229. Cell lines & gene of interest - (reply: 1)
  230. Software for selection genes related to concrete cell processes - (reply: 1)
  231. a gene expressed in 293 cell lines but not Hela cells - (reply: 1)
  232. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)
  233. How genes are selectively silenced ? - (reply: 1)
  234. real time qRT-PCR and low expression gene - (reply: 2)
  235. Cloning genes into plant vector - (reply: 1)
  236. PCRing mature form of a gene - (reply: 1)
  237. Gene Expression Issues - (reply: 3)
  238. No gene amplification, 18s amplification is fine - (reply: 1)
  239. Cloning large gene - (reply: 3)
  240. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  241. Confirmed gene knockdown not showing on array - (reply: 2)
  242. how to determine fold change of gene expression - (reply: 2)
  243. negative values after GOI - reference gene ?? - (reply: 4)
  244. Gene expression change after mutagenesis-- please help! - (reply: 4)
  245. Two start codons" the TAG and the gene" - (reply: 1)
  246. expreesing cloned vector - having problems with texpreesing cloned gene in vector. (reply: 6)
  247. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
  248. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
  249. Gene on negative strand - two eductional questions. - (reply: 3)
  250. 3'UTR sequence of a gene - (reply: 3)
  251. Enriched gene signatures - (reply: 1)
  252. how does the binding of miRNA at 3'UTR regulates the gene - (reply: 3)
  253. Sigma esiRNA induces target gene expression - (reply: 1)
  254. High resolution melting to gene scanning - (reply: 1)
  255. Is one reference gene ever appropriate? - (reply: 3)
  256. Trouble to UNDERSTAND gene replacement results - (reply: 1)
  257. Comprehensive database for all the known epigenetic genes in human and mouse - (reply: 2)
  258. Restriction Enzyme sites in my genes - (reply: 1)
  259. infection with two retroviruses? - Delivery of two Genes in distinct virus constructs, does it work? (reply: 3)
  260. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
  261. qPCR with low expressing genes - (reply: 1)
  262. Reference genes. - (reply: 1)
  263. Reference genes - (reply: 2)
  264. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
  265. Problem cloning wild type E. coli gene... Possible recombination event in DH5alp - (reply: 4)
  266. Gene set similarity searches in public databases - (reply: 4)
  267. Gene copy number analysis - (reply: 1)
  268. reference gene - (reply: 2)
  269. Predicting function of heterozygous vs WT gene? - (reply: 1)
  270. is there a method to set up stable transfectants without drug selection - no drug-resistant gene (reply: 8)
  271. Selection of housekeeping gene in Mycobacterium - (reply: 1)
  272. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
  273. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
  274. finding exons for a gene, having the coding sequence only - (reply: 4)
  275. New to Gene cloning - Plasmid vector preparation (reply: 2)
  276. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  277. Regression analysis between two genes - (reply: 1)
  278. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
  279. copy number of gene in genome - (reply: 4)
  280. Cloning of a gene containing 31 exon - (reply: 1)
  281. predict union between microRNAs and genes - (reply: 2)
  282. A gene is not inserted into the Plasmid - (reply: 2)
  283. Reporter gene to test new promotor - (reply: 1)
  284. How to study with the candidate genes? - (reply: 2)
  285. Delta Delta Ct method for multiple reference genes? - (reply: 1)
  286. Cloning a foreign gene into E-coli - (reply: 1)
  287. virus with human gene to mouse cell - Does it work? (reply: 2)
  288. Transfecting a cancer gene - (reply: 2)
  289. housekeeping genes in PCR - (reply: 1)
  290. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
  291. gene expressional analysis - (reply: 1)
  292. Biotinylation sequence: gene orientation?? - (reply: 1)
  293. Can every gene be methylated? - (reply: 1)
  294. Antibody gene cloning - (reply: 3)
  295. Gene not expressed in wild-type and knockout yet differences in microarray - (reply: 15)
  296. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  297. Gene targeting - (reply: 3)
  298. Cheap procedure for gene deletion in E coli? - (reply: 2)
  299. integration of a gene per homologous recombination - (reply: 5)
  300. Ki-67 - no difference bwteen tumor suppressor gene vs ctrl (reply: 3)
  301. relative comparison of many samples and genes in qRT-PCR - (reply: 1)
  302. Reference genes and dilutions in qPCR - (reply: 1)
  303. Molecular identification of ESBL genes blaGES-1, blaVEB-1, blaCTX-M blaOXA-1, bl - International Journal Biological & Life Sciences. 6 (3): 138-142, (reply: 1)
  304. [Software] Drawing arrows as ORFs on genes - (reply: 6)
  305. cre-loxP: how to check if the gene is expressed - (reply: 3)
  306. cre-loxP: how to check if the gene is expressed - (reply: 8)
  307. producing recombinant -one gene plus strand/other gene complemenary and reverse - (reply: 3)
  308. Two genes that are contiguous in the genome... - ...the relationship between them (reply: 7)
  309. clone GENE RT-PCT PRIMERS - cloning genes (reply: 1)
  310. Ghost gene - (reply: 3)
  311. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  312. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
  313. trouble with protein expression of high AT rich gene - (reply: 1)
  314. Cytosolic Housekeeping gene - (reply: 1)
  315. Gene signatures in breast cancer - (reply: 2)
  316. How to delete a domain in a gene? - cloned plasmid (reply: 12)
  317. cloning more than 1 gene into vector if no IRES available - Xenopus IRES? if not, what to do? (reply: 2)
  318. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
  319. How to download the complete list of genes in a chromosome(chromosome 19) - finding complete list of genes in a chomosome in NCBI map viewer (reply: 7)
  320. help with ct of reference gene - (reply: 1)
  321. colony PCR for subcloning gene - (reply: 1)
  322. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  323. siRNA sequence can be used as shRNA? - Stable down regulation of target gene expression. (reply: 1)
  324. how long will it takes to silence target gene after induction of shRNA - (reply: 2)
  325. how to find kappaB binding site on the promoter of a gene - kappaB binding site in gene promoter (reply: 2)
  326. What is the BGH gene for in a expression vector? - What is the BGH gene for in a expression vector? Thanks a lot! (reply: 4)
  327. Links between genes and risk factors in polygenic diseases - (reply: 2)
  328. Does a viral gene have introns? - (reply: 4)
  329. Blast Nucleotide Issue - No more Gene ID when comparing Bacteria genome (reply: 3)
  330. Gene expression confusion - (reply: 5)
  331. Retroviral Vector Construction - Simultaneous gene expression and shRNA knockdown? (reply: 8)
  332. use of housekeeping gene in RT PCR chIP - (reply: 3)
  333. Gene cross-checking - (reply: 14)
  334. Using Gene Ontology? - (reply: 1)
  335. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
  336. High Gene low protein levels - (reply: 1)
  337. stable clones - correlation between 2 genes in the same family (reply: 1)
  338. how to find TSS of a certain gene - (reply: 1)
  339. How to get a list of all variants (SNPs) within a gene and 5kb immediately 5' - (reply: 3)
  340. About gene orientation - (reply: 2)
  341. very high and inconsistent Cts for the house keeping gene - (reply: 4)
  342. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
  343. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
  344. Doubt with recombinant expression genes from streptobacillus moniliformis and Er - (reply: 2)
  345. Looking for FASTA format of DNA of a gene amongst various species - (reply: 7)
  346. House keeping gene question? - Thanks for any help! (reply: 1)
  347. Problems with validating primers and low expression genes - (reply: 3)
  348. Deep sequencing to find gene targets of protein? - (reply: 1)
  349. How can multiple cloning sites (MCS) NOT disrupt lacZ gene? - (reply: 2)
  350. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
  351. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
  352. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
  353. Gene expression of methylated gene - (reply: 4)
  354. GAPDH CT lower than target gene CT - (reply: 2)
  355. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
  356. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
  357. mutagenize plasmid-encoded gene - (reply: 4)
  358. Sequencing of gene promoter/terminator, when you only have the AA sequence of th - (reply: 2)
  359. Fusion of reporter genes - (reply: 2)
  360. Cloning from cDNA - the wrong gene?! - (reply: 2)
  361. failure PCR amplification from low GC content gene - (reply: 5)
  362. How many copies of the gene - (reply: 1)
  363. Antibiotic resistance genes in a plasmid - How are they expressed? (reply: 3)
  364. Measuring gene expression across cell lines - (reply: 2)
  365. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
  366. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
  367. How to find gene homologues? - (reply: 1)
  368. microRNAs and mRNAs. Interpreting gene expression profiles - Gene expression profiling and microRNAs (reply: 4)
  369. complete gene for expression? - (reply: 2)
  370. questions on gene therapy in brain - (reply: 1)
  371. 5' RACE for very GC-rich gene - (reply: 2)
  372. How to remove copy of redundant cDNA in a mixture - RT results in a cDNA library with high copy number of few genes (reply: 3)
  373. Large Difference between Ct of 18s rRNA and Target Gene - (reply: 2)
  374. Isoforms of a gene!! - how to find all isoforms of a gene using bioinfo tools?! (reply: 2)
  375. cloning - how to clone a gene having stop codons (reply: 4)
  376. how to intentionally alter gene sequence - (reply: 1)
  377. Relatively unknown gene, need approach suggestions - (reply: 1)
  378. Why is the y-axis labeled 'relative gene expression'? - (reply: 5)
  379. how to express the gene in opposite transcriptional direction - (reply: 3)
  380. How could I express two genes in opposite orientation - (reply: 2)
  381. always run internal control for different genes on the differnet plates? - (reply: 8)
  382. Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable (reply: 1)
  383. help needed: PCR a gene from genomic DNA - (reply: 2)
  384. cloning of cellulose synthase gene - (reply: 1)
  385. Absolute quantification and normalization - Are reference genes necessary? (reply: 1)
  386. Where can you get the sequence of one exon of a gene? - (reply: 3)
  387. Validation of housekeeping gene expression - (reply: 3)
  388. GMO's - introduction of herbicide resistance gene (reply: 3)
  389. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
  390. software for gene ontology - (reply: 2)
  391. Rotor Gene 3000 and Rotor Gene Q - (reply: 1)
  392. designing primers for genes not sequenced yet - (reply: 2)
  393. Expression of a possible toxic gene? - (reply: 5)
  394. get data from gene chip - (reply: 2)
  395. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  396. How to compare different expression levels between genes - (reply: 1)
  397. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  398. miRNA gene UTRs? - (reply: 3)
  399. reference genes for qPCR - (reply: 3)
  400. Q about the housekeeping gene - (reply: 1)
  401. deleting a certain gene from bacterium - (reply: 12)
  402. promoter of a gene - (reply: 2)
  403. searching housekeeping genes - (reply: 4)
  404. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
  405. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
  406. gene copy number calculation - qPCR (reply: 2)
  407. Reference gene qPCR - (reply: 4)
  408. doubt in gene annotation - doubt in submitting gene annotation to NCBI (reply: 1)
  409. relative gene expression with multiple reference genes - data analysis (reply: 1)
  410. How to detect a gene having multiple isoforms using southern/northen techniques - (reply: 1)
  411. What Factors Affect Ct values of a gene? - (reply: 1)
  412. Can linear vectors be transformed and can they express resistance genes? - (reply: 4)
  413. how to test if a given microRNA regulates a gene of interest - microrna target (reply: 7)
  414. how to know a sequene for a specific gene & produce it - (reply: 5)
  415. Gene insertion without desired unique restriction sites - (reply: 3)
  416. Gene cluster drawing software - Anythign to draw genes cluterested together like in an operon (reply: 1)
  417. How to find upstream genomic sequence of a gene? - (reply: 2)
  418. Gene expressed in lenti-packaging cells, but not in lenti-infected cells - (reply: 2)
  419. Anyone using AppBiosys TaqMan Gene Expression Assays in LightCycler or RotorGene - (reply: 2)
  420. getting crazy about a gene bank entry - (reply: 2)
  421. Need Help: Bacterial gene deletion- sacB sucrose resistance - (reply: 31)
  422. Problems with gene knockdown - No knockdown with validated shRNAs :( (reply: 6)
  423. problem in gene cloning - (reply: 4)
  424. Trouble with GAPDH housekeeping gene - (reply: 3)
  425. Cloning a whole gene in pBAD (any comments pls) - (reply: 4)
  426. HELP: gene with intron expressed in Pichia - (reply: 2)
  427. Promoter/gene cloning question - (reply: 7)
  428. Gene Ontology - Looking for a good software (reply: 3)
  429. Gene screening - (reply: 2)
  430. Gene Expression analysis - (reply: 4)
  431. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  432. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
  433. troubleshooting: gene deletions by method of Wanner and Datsenko - (reply: 2)
  434. yeast transformation - Expression of plant genes in yeast (reply: 1)
  435. Real time house keeping genes change - (reply: 1)
  436. A MAPKKK gene, receptor or not? - (reply: 3)
  437. What genes can be used to validate H3K27me3-ChIP - (reply: 8)
  438. Tool for design gene structure figures - (reply: 5)
  439. how to determine certain gene copy number? - (reply: 3)
  440. expressing data with more than 1 HK gene - (reply: 2)
  441. Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) - Can it make them sick? They're small and take forever to grow. (reply: 2)
  442. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  443. known gene or area of a chromosome - help (reply: 1)
  444. Amplifying a gene using a degenerate primer - (reply: 4)
  445. Chosing the best housekeeping gene! - (reply: 5)
  446. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
  447. need help on isolation of intron of gene - (reply: 1)
  448. SiRNA- gene silencing in brain Organotypic slice cultures - (reply: 3)
  449. Gene expression in cell line - (reply: 1)
  450. Is H3K4 trimethylation always associated with gene activation? - (reply: 1)
  451. house keeping gene in transgenic mice. - (reply: 1)
  452. Detect gene in stable cell line, - (reply: 4)
  453. selection of internal control gene - (reply: 2)
  454. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
  455. GFP reporter gene - (reply: 3)
  456. Gene Bridges Red/ET cloning - unexpected streptomycin resistance (reply: 4)
  457. Bacteria gene expression - (reply: 2)
  458. minimum length for the gene to be amplified in PCR - (reply: 6)
  459. wrong gene - (reply: 13)
  460. gene design - restriction site addition (reply: 2)
  461. Number of genes and number of proteins in humans - (reply: 1)
  462. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
  463. Understanding qPCR and gene expression changes - (reply: 10)
  464. zebrafish gene regulation can I use mammel expression vector? - (reply: 1)
  465. Heterogenous expresion of stably transfected gene - (reply: 4)
  466. assembling problem in genes - assembling problem in genes (reply: 7)
  467. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  468. Promoters and gene expression - (reply: 3)
  469. Automated sequencing - Difficult gene (reply: 1)
  470. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  471. Gene knockin or knockout? transgenic mouse - (reply: 2)
  472. want gene knock out protocol - (reply: 2)
  473. Target gene regulation by transiently transfected PR - (reply: 4)
  474. Mycobacterial housekeeping genes - (reply: 1)
  475. Transfer of gene from one vector to another vector - (reply: 2)
  476. Macroarray platforms - CGH vs Gene Expression (reply: 4)
  477. Houskeeping Gene not steady - (reply: 7)
  478. candidate gene approaches - (reply: 3)
  479. size of gene insert wrong? - (reply: 3)
  480. size of gene insert vs. predicted size - (reply: 1)
  481. Good SiRNA Gene silencing - (reply: 1)
  482. yeast gene deletion - Could I delete an essential gene from haploid cells? (reply: 8)
  483. deleting lacZ gene from control vector - (reply: 5)
  484. methylation by MSSS1(NEB )for reporter gene analysis - (reply: 2)
  485. How to design an experiment to prove that plasmid do contain genes coding for va - (reply: 6)
  486. RNAi in bacteria - Why no finding for gene silencing in bacteria? (reply: 1)
  487. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)