About gene orientation - (Jun/07/2010 )
Hi, i just wanna know what is the effect of gene orientation in a recombinant construct on protein synthesis. Consider following two cases.
in case 1 expression will proceed in two directions and therefore read-through transcription from one transcript to the other will be prevented.
in case 2 expression goes in one direction and although there is a terminator after GOI1 there will be read-through transcription from promoter1.
I am just wondering if there is a terminator in between, then how a read through transcript is possible originating from promoter 1.
Another question is what if i use same promoter for both transcripts in case 1. Will there be any steric hinderance?