selection of internal control gene - (Apr/29/2009 )
I am going to do qPCR. My target gene is expressed at a very low level. Can I use housekeeping genes? I was told that comparable CTs between target and control are preferred, but can't find any official saying about this.
makiyo on Apr 29 2009, 11:45 AM said:
It is more important that your amplification efficiencies for both genes (target and control) are similar, rather than their absolute Ct values. Some of the housekeeping genes should be fine.
I would suggest using more than 1 house keeping gene just so that the results donot mislead you.