microRNAs and mRNAs. Interpreting gene expression profiles - Gene expression profiling and microRNAs (Feb/24/2010 )
Hi guys,
I'm a newbie and may be I'm doing a stupid question but I really need to understand. I will do an example to make me understand. Let say that in a gene expression profiling I see that a given mRNA is equally upregulated in a set of samples. At the same time I know that only in one of these samples are present the microRNAs (let say 4) that are potentially targeting it (targetscan prediction). May I say that likely in this sample I don't have the protein even though the mRNA level is high? Thank you in advance for your help....
-netdavid-
netdavid on Feb 24 2010, 11:05 AM said:
Hi guys,
I'm a newbie and may be I'm doing a stupid question but I really need to understand. I will do an example to make me understand. Let say that in a gene expression profiling I see that a given mRNA is equally upregulated in a set of samples. At the same time I know that only in one of these samples are present the microRNAs (let say 4) that are potentially targeting it (targetscan prediction). May I say that likely in this sample I don't have the protein even though the mRNA level is high? Thank you in advance for your help....
I'm a newbie and may be I'm doing a stupid question but I really need to understand. I will do an example to make me understand. Let say that in a gene expression profiling I see that a given mRNA is equally upregulated in a set of samples. At the same time I know that only in one of these samples are present the microRNAs (let say 4) that are potentially targeting it (targetscan prediction). May I say that likely in this sample I don't have the protein even though the mRNA level is high? Thank you in advance for your help....
I wouldn't say "likely". Let's say remotely possible but yes, you could think that. But always beware of target prediction, it is VERY far from being reliable.
If there are not known miRNAs targeting your mRNA you have nothing else to do but try.
Fiz
-Fizban-
Fizban on Feb 24 2010, 07:42 AM said:
netdavid on Feb 24 2010, 11:05 AM said:
Hi guys,
I'm a newbie and may be I'm doing a stupid question but I really need to understand. I will do an example to make me understand. Let say that in a gene expression profiling I see that a given mRNA is equally upregulated in a set of samples. At the same time I know that only in one of these samples are present the microRNAs (let say 4) that are potentially targeting it (targetscan prediction). May I say that likely in this sample I don't have the protein even though the mRNA level is high? Thank you in advance for your help....
I'm a newbie and may be I'm doing a stupid question but I really need to understand. I will do an example to make me understand. Let say that in a gene expression profiling I see that a given mRNA is equally upregulated in a set of samples. At the same time I know that only in one of these samples are present the microRNAs (let say 4) that are potentially targeting it (targetscan prediction). May I say that likely in this sample I don't have the protein even though the mRNA level is high? Thank you in advance for your help....
I wouldn't say "likely". Let's say remotely possible but yes, you could think that. But always beware of target prediction, it is VERY far from being reliable.
If there are not known miRNAs targeting your mRNA you have nothing else to do but try.
Fiz
Thank you Fiz for your reply........I just got some results.......only in one sample these microRNAs are present. So now I have high mRNA expression in all the sample but and high microRNA expression (that supposedly targeting it) only in one of them (which of course behave differently). Unfortunately no antibodies are available for this protein. What should I do in your opinion? Is an shRNA against this mRNA a resonable approach? Thank you again
-netdavid-