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finding gene sequence to delete - (Feb/15/2013 )

Please if you can help.

My task is to find/locate a gene for oxoglutarate dehydrogenase (ketoglutarat dehydrogenase) in Streptomyces lividans or in Streptomyces coelicolor. I need a nucleotide sequence in order to delete it.

I have worked in human genetic lab till now and I am not familiar with this bacterial genome at all... Which page to use (I am used to ensembl for humans) to get a gene nt seq and locate it in bacterial genome?

Please help....


Here is a link.

In this link there is no Streptomyces lividans, but if the sequence is similar to the other streptomyces species in this link you can use that. I am working with E. coli. Usually we use different E. coli sequences. Mostly same genus carries almost similar sequences. I found your ketoglutarate dehydrogenase in Streptomyces coelicolor, 1272 aminoacids.

Best of luck,



Thanks Christy! You are my savior!



thanx neuron. ;-)