finding gene sequence to delete - (Feb/14/2013 )
Please if you can help.
My task is to find/locate a gene for oxoglutarate dehydrogenase (ketoglutarat dehydrogenase) in Streptomyces lividans or in Streptomyces coelicolor. I need a nucleotide sequence in order to delete it.
I have worked in human genetic lab till now and I am not familiar with this bacterial genome at all... Which page to use (I am used to ensembl for humans) to get a gene nt seq and locate it in bacterial genome?
Here is a link.
In this link there is no Streptomyces lividans, but if the sequence is similar to the other streptomyces species in this link you can use that. I am working with E. coli. Usually we use different E. coli sequences. Mostly same genus carries almost similar sequences. I found your ketoglutarate dehydrogenase in Streptomyces coelicolor, 1272 aminoacids.
Best of luck,
Thanks Christy! You are my savior!
thanx neuron. ;-)