need help on isolation of intron of gene - (May/21/2009 )
I want to isolate intron of LIN6 gene of tomato to make RNAi experiment and i have total gene sequence available in genebank. Can anyone help me? how to isolate intron of my gene with PCR.
Thanks in advance,
get the exon (coding) sequence and align with the full genomic sequence. This will give you the intron/exon boundaries which you can design primers around.