Reference genes - (May/25/2011 )
I have a question about reference genes. Why when I design assay for some gene the primer sequence are different when I use different reference gene. I thought that primer sequences for my target gene are the same no matter which reference i use.
the answer lays down in your question !
u are comparing different genes ... so ,by default, there will be different primers ...
I'm comparing different reference genes with the same target gene and primer sequences are different for the target gene depending on reference gene. I don't change target gene but I change reference gene and then I have different primers for my target gene.