Selection of housekeeping gene in Mycobacterium - (Mar/31/2011 )
I am now currently working on detection of mRNA level on Mycobacterium Smegmatis, are there any good choice for the housekeeping gene??? I cant search for a good one. Thank you.
-jeff1234-
Hi, maybe its helpful. in this paper they used elongation factor Tu and they used it for mycoplasma detection but it could be that it also works as reference gene.
St�rmer M, Vollmer T, Henrich B, Kleesiek K, Dreier J. Broad-range real-time
PCR assay for the rapid identification of cell-line contaminants and clinically
important mollicute species. Int J Med Microbiol. 2009 Apr;299(4):291-300. Epub
2008 Oct 15. PubMed PMID: 18926769
-tea-test-