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reference genes for qPCR - (Nov/18/2009 )

Hi,

i'm doing qPCR and know that you have to take some normalization genes in each run.
I wondered which genes, are usually used.
Do somebody has some experience with this? And which genes, did you use?

greetz

-susanna-

susanna on Nov 18 2009, 11:03 PM said:

Hi,

i'm doing qPCR and know that you have to take some normalization genes in each run.
I wondered which genes, are usually used.
Do somebody has some experience with this? And which genes, did you use?

greetz



Hi

you should take a housekeeping gene/constitutive gene for normalization purposes...Genes which would remain unaffected even with different conditions used. ppl use actin, GAPDH etc....i use a mitochondrial ribosomal subunit......depends on the system that you are working on....

Best....

-DRN-

DRN on Nov 18 2009, 05:52 PM said:

depends on the system that you are working on....

True.
Actin usually works fine but it's not a good choice if your RNA samples weren't treated with DNase before.
Besides, pbgd and h-mrpl, from those that I know.

-unity-

unity on Nov 19 2009, 10:58 AM said:

DRN on Nov 18 2009, 05:52 PM said:

depends on the system that you are working on....

True.
Actin usually works fine but it's not a good choice if your RNA samples weren't treated with DNase before.
Besides, pbgd and h-mrpl, from those that I know.


yes, i'm using DNase before.
Thanks for the reply!

-susanna-