Different annealing temp between HKG and target gene - (Feb/25/2014 )

Dear all,

I want to ask whether it is okay to run absolute quantification if the housekeeping gene's and target gene's annealing temperature were different. The target gene's is 65 C and the hkg gene's is 60 C.

Thank you.

Cheers,

dee

Yes.

As long as you run all the standards for each gene on the same plate/run/temperature.

Thank you Trof.