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Best way to genotpy a mutant gene with a 1bp difference. - (Jul/19/2012 )

What is the best way to genotype a mutant gene with a 1bp difference? I'm trying to check whether my DNA is homozygous or heterozygous mutant but I'm having some difficulties due to the tiny difference.

Thanks

-Wek-

You could either do sequencing (most sensitive) or perhaps dHPLC if you have one available.

-bob1-

Is there any protocol to optimize the DNA for sequencing?

-Wek-

You may be able to RE cut the fragment to make it short, e.g. 25 bp. The 1 bp difference would then be far easier to see on a high concentration page gel.

-phage434-