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Gene knockin or knockout? transgenic mouse - (Mar/13/2009 )

Hello friends,

I identified a gene to be a potential oncogene, ie, high expression in cancer cell lines and tissues, its knockdown by RNAi causes inhibition of cell growth, its overexpression causes transformation. Now I want to further study the function of this gene by creating transgenic mice. Should I create knockin mice or knockout mice?

Thanks for your input!


I believe knockin of a suspected oncogene makes more sense, what do you think?


I think it depends on what do you want to know........
Knockout ===> e.g. affect (embryonic) development? or it just (specifically) inhibits cancer cell growth?
knockin ====> use tissue specific promoter, for example epoR promoter, will you see anything in hematopoietic progenitor cells?
Just my two cents.

-Functional Screens-