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want gene knock out protocol - (Mar/12/2009 )

hi


i am not able to find a protocol for gene knock out even after a through search,
please any one help me in this regard,i would be very thankful to them.

thanks in advance.

waiting for some response.

-bablu70760-

bablu70760 on Mar 13 2009, 06:37 AM said:

hi


i am not able to find a protocol for gene knock out even after a through search,
please any one help me in this regard,i would be very thankful to them.

thanks in advance.

waiting for some response.


You'll have to give us more information. What organism do you want to knock a gene out of?

Clare

-Clare-

Clare on Mar 13 2009, 02:56 AM said:

bablu70760 on Mar 13 2009, 06:37 AM said:

hi


i am not able to find a protocol for gene knock out even after a through search,
please any one help me in this regard,i would be very thankful to them.

thanks in advance.

waiting for some response.


You'll have to give us more information. What organism do you want to knock a gene out of?

Clare


thank god there is some one to listen and help me out there.
first of all thank you for your reply"CLARE" :lol:
Ok now here i go
i have started working on this(gene knock out) from 4 days,i am just a beginner,so obviously my organism is E.coli the strain may be mostly DH5alpha and the knock out gene is dehydrogenase.
And i have found that lambda red recombinase method is used for knocking out the gene.
How would i get an good protocol so that i can start my work and do it uninterruptedly.
thank you once again Clare.

eagerly waiting for reply.....

-bablu70760-