gene copy number calculation - qPCR (Oct/27/2009 )
Hi,
Can someone brief me on hoe to calculate gene copy number using qPCR. What sample do I use for standard? I'm checking the expression f mrna from serum samples from a articular cancer. So, do I use teh patient sera for the standard?
-SF_HK-
SF_HK on Oct 27 2009, 02:35 PM said:
Hi,
Can someone brief me on hoe to calculate gene copy number using qPCR. What sample do I use for standard? I'm checking the expression f mrna from serum samples from a articular cancer. So, do I use teh patient sera for the standard?
Can someone brief me on hoe to calculate gene copy number using qPCR. What sample do I use for standard? I'm checking the expression f mrna from serum samples from a articular cancer. So, do I use teh patient sera for the standard?
hope this will help u!!!!
find the attachment!!!
-Pradeep Iyer-
Pradeep Iyer on Oct 27 2009, 04:45 PM said:
SF_HK on Oct 27 2009, 02:35 PM said:
Hi,
Can someone brief me on hoe to calculate gene copy number using qPCR. What sample do I use for standard? I'm checking the expression f mrna from serum samples from a articular cancer. So, do I use teh patient sera for the standard?
Can someone brief me on hoe to calculate gene copy number using qPCR. What sample do I use for standard? I'm checking the expression f mrna from serum samples from a articular cancer. So, do I use teh patient sera for the standard?
hope this will help u!!!!
find the attachment!!!
Thank you very much
-SF_HK-