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gene copy number calculation - qPCR (Oct/27/2009 )

Hi,

Can someone brief me on hoe to calculate gene copy number using qPCR. What sample do I use for standard? I'm checking the expression f mrna from serum samples from a articular cancer. So, do I use teh patient sera for the standard?

-SF_HK-

SF_HK on Oct 27 2009, 02:35 PM said:

Hi,

Can someone brief me on hoe to calculate gene copy number using qPCR. What sample do I use for standard? I'm checking the expression f mrna from serum samples from a articular cancer. So, do I use teh patient sera for the standard?


hope this will help u!!!!
find the attachment!!!
Attached File

-Pradeep Iyer-

Pradeep Iyer on Oct 27 2009, 04:45 PM said:

SF_HK on Oct 27 2009, 02:35 PM said:

Hi,

Can someone brief me on hoe to calculate gene copy number using qPCR. What sample do I use for standard? I'm checking the expression f mrna from serum samples from a articular cancer. So, do I use teh patient sera for the standard?


hope this will help u!!!!
find the attachment!!!

Thank you very much

-SF_HK-