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Antibody Related Discussions
  1. Raising monoclonal antibodies - Monoclonal antibodies raised against similar sequence Antigens in mice (reply: 2)
  2. antibody titer - (reply: 1)
  3. which part of an antigen should monoclonal antibodies bind to? - how to choose appropiate epitopes? (reply: 1)
  4. Co-immunoprecipitation - Source of cheap irrelevant antibody (reply: 2)
  5. Antibody out of stock: ab8580 - (reply: 4)
  6. Secondary antibody - (reply: 2)
  7. antibody against His-tagged protein - (reply: 3)
  8. Visualizing CpG island in IF - Are there antibodies available? (reply: 1)
  9. mAB vs pAB - (reply: 4)
  10. Non-specific bands with secondary Antibody on Western Blotting - (reply: 1)
  11. Blocking antibody - (reply: 1)
  12. HSV1-Tk (mono/ployclonal) antibody. - (reply: 1)
  13. anyone knows good HA antibody for zebrafish whole mount stainging - (reply: 4)
  14. Antigen coated beads - For making antibodies (reply: 1)
  15. antibody to use for methylase chip - DNMT/MBD/MeCP2? (reply: 3)
  16. Immobilisation of Antibodies to Au ( Gold) surface - (reply: 2)
  17. antibodies working in indirect ELISA but not in inhibition ELISA - (reply: 11)
  18. Bacterial expression antibody recognition problem - antibodies won't recognize protein (reply: 2)
  19. Why do I get non-specific bands? - with my secondary antibody.... (reply: 5)
  20. How to release antibodies from beads after IP? - in order to run ELISA after IP, not Western Blot. (reply: 7)
  21. Negative control for primary antibody in immunohistochemistry - What is it? (reply: 9)
  22. Coating with monoclonal/polyclonal - (reply: 6)
  23. Digestion of antibody using pepsin - no F(ab)2 was found (reply: 1)
  24. Control for primary antibody in Immunohistochemistry - What is it? (reply: 4)
  25. recombinant human antibody production problem - (reply: 2)
  26. How to reduce amount of antibodies used in WB - (reply: 4)
  27. Monoclonal vs Polyclonal Abs - (reply: 1)
  28. Resuspending lyophylized antibodies - (reply: 1)
  29. antibody storage - how to store? (reply: 7)
  30. Custom polyclonal peptide antibody services? - (reply: 4)
  31. gel and monoclonal antibody issues - (reply: 3)
  32. How to reuse antibody in Western blotting - Share your experience (reply: 8)
  33. Is my antibody specific? - (reply: 3)
  34. Can we reuse the primary antibody in immunohistochemistry? - (reply: 3)
  35. Testing the primary antibody - Is the secondary antibody recognizing the primary (reply: 1)
  36. Polyclonal antibody service - Who can you recommend? (reply: 2)
  37. Concentrating antibodies - (reply: 3)
  38. What is needed for a hybridoma growth culture - (reply: 1)
  39. Santa Cruz antibody - (reply: 14)
  40. Help: New to biochemistry and need help with antibody? - (reply: 11)
  41. affinity determination of an antibody - (reply: 5)
  42. Hybridoma cultures - floaters? - (reply: 4)
  43. polyclonal vs monoclonal - (reply: 2)
  44. Can I incubate membrane with 2 antibodies at the same time ? - (reply: 5)
  45. Western Blot Background - Having high backgrounds on western blotting due to antibody issues. (reply: 2)
  46. crosslinking antibodies before IP - (reply: 2)
  47. mouse anti-goat antibodies - (reply: 1)
  48. Monoclonal antibody affinity (Kd constant) determination using ELISA - (reply: 2)
  49. Can an antibody just stop working? - (reply: 5)
  50. HA antibody - (reply: 1)
  51. Diluting/mixing of antibodies - exact method? - (reply: 1)
  52. Blocking for phospsho antibodies - (reply: 4)
  53. Apoptosis detection with Phosphatidylserine antibody - (reply: 4)
  54. Frozen Antibody Lifespan - (reply: 2)
  55. anti-gfp antibodies - (reply: 4)
  56. Hybridoma culture not secreting mouse antibody - (reply: 6)
  57. mouse anti-mouse antibody ? - (reply: 5)
  58. Which p-Serine antibody is suitable for IP and WB - (reply: 1)
  59. "recombinanant antibody" - (reply: 3)
  60. How about the antibody quality of these two companies? - antibody quality (reply: 5)
  61. HELP! confusing antibody - (reply: 14)
  62. Denaturation of antibodies in high salt - (reply: 3)
  63. problem with anti-acetyl H3 antibody - (reply: 11)
  64. IP problems - The antibody doesn't bind to the beads (reply: 5)
  65. polyclonal antibodies go bad - when stored at -80???? (reply: 5)
  66. shape-shifting antibodies? - (reply: 11)
  67. Making antibodies - Anybody has designed a custom antibody? (reply: 4)
  68. stripping antibodies in immunochemistry? - (reply: 6)
  69. antibody against yeast protein - (reply: 1)
  70. Antibodies for immunohistochemistry and cell ELISA - Are they the same? (reply: 2)
  71. antibody classification - (reply: 1)
  72. storage of ammonium sulphate purified antibodies - (reply: 1)
  73. Antibodies for different epitope giving bands of different sizes in the same sam - (reply: 1)
  74. Do antibody solutions settle? - (reply: 2)
  75. creating a neg. charge on an Antibodies' Fc region - (reply: 2)
  76. Why should some antibodies not be aliquoted? - (reply: 12)
  77. RNA polymerase II antibody recommendation? - (reply: 2)
  78. Mab purification - no mouse IgG band seen (reply: 3)
  79. Monoclonal screening of stable cells - How to maintain it to confluent stage? (reply: 4)
  80. macrophages collection for hybridoma - (reply: 1)
  81. Couple peptide to beads? for antibody purification - (reply: 1)
  82. Looking for an antibody/epitope type of search... - (reply: 1)
  83. polyclonal antibody production - is it necessay to remove fusion tag? (reply: 2)
  84. pSTAT5 antibody - (reply: 5)
  85. Ki67 Antibodies - (reply: 6)
  86. Antibody detecting albumin - (reply: 1)
  87. lyophilization of monoclonal antibodies... How? - (reply: 5)
  88. Affinity purification of antibody - (reply: 2)
  89. conformation specific monoclonal antibody - how to confirm? (reply: 4)
  90. antibody purification - how to get rid of bovine IgG? (reply: 3)
  91. Antibodies for ChiP - Of mice and men (reply: 1)
  92. polyclonal antibody production protocol - looking for protocols (reply: 2)
  93. monoclonal antibody production - (reply: 6)
  94. Human TERT Antibody - (reply: 2)
  95. humanizing an antibody? - (reply: 5)
  96. Polyclonal antibodies in immunocytochemistry not working - (reply: 4)
  97. Multiple antibodies in Western - (reply: 5)
  98. Are there antibodies to differentiate epithelial from fibroblasts cells? - (reply: 5)
  99. Rainbow MW marker from Amersham - detection with a mouse secondary antibody? (reply: 1)
  100. EMSA witj antibodies against TFII factors - (reply: 3)
  101. c-Myc antibody for Western - (reply: 1)
  102. Antibody concepts - (reply: 20)
  103. antibodies life - 4oC, -20oC or -80oC (reply: 26)
  104. Antibody produced in same species as target tissue - Will it cross react? (reply: 2)
  105. Western Blotting using Anti α Tubulin antibody: Bands too high! - (reply: 3)
  106. ChIP IgG has the same signal than the antibody tested - (reply: 9)
  107. NeuN and Ox-42 antibodies - (reply: 4)
  108. antibody against denatured protein? - basic questions (reply: 2)
  109. Immunofluorescence using polyclonal antibody - (reply: 5)
  110. monoclonal ab recognize one or more epitopes? - (reply: 4)
  111. Smearing antibody and DTT - how to interpret the gel? - Aggregated antibody (reply: 1)
  112. Antibody Mismatch Tolerance - (reply: 2)
  113. Problem in IP (too high background) - mAb shows a lot of backgroud+light chain. (reply: 3)
  114. estimation of concentration of Ab in hybridoma culture supernatant? - (reply: 4)
  115. can u guess the molecular weight of a raised antibody? - (reply: 3)
  116. dilution buffer for ap labeled 2nd antibody - (reply: 1)
  117. does 2nd antibody concentration matters? - (reply: 3)
  118. same proteins, same antibodies, different results - (reply: 1)
  119. Shipping antibodies - (reply: 4)
  120. Western blottting - Santacruz antibodies - (reply: 10)
  121. Can anyone recommend the best FLAG and c-myc antibodies for co-IP? - (reply: 4)
  122. Anybody has a good phosphorylated IKKa antibody? - (reply: 3)
  123. ASK1 antibody - (reply: 2)
  124. Antibody purification protocol --any suggestions? - (reply: 12)
  125. oligopeptide beased antibody for IP - (reply: 2)
  126. vortexing of antibodies upon dilution in 3% skim milk - (reply: 3)
  127. antibody concentration - antibody precipitation using ammonium sulfate (reply: 2)
  128. Concentration of antibody - (reply: 5)
  129. specific ser/Thr phosphorylation detection by in house antibody - (reply: 1)
  130. humanized antibody - how to design primers for CDRs - humanized antibody (reply: 1)
  131. Antibody question - (reply: 5)
  132. Polyclonal antibody purification? - how? (reply: 9)
  133. How can I elute antigen from proteinG+antibody+antigen complexes? - (reply: 3)
  134. questions about secondary antibody - (reply: 2)
  135. Rabbit IgG Isotype Control for Co-ip - Need available antibodies (reply: 1)
  136. Problem with antibodies - (reply: 10)
  137. antibody question - (reply: 7)
  138. can antibodies be added to cell culture for 10d before stain - immunocytochemistry (reply: 1)
  139. someone has frozen my antibody, is it still alive? - (reply: 2)
  140. Using denaturing protcol for his-protein purification - Can it still be used for antibody production????? (reply: 4)
  141. making a monoclonal from scratch... - (reply: 1)
  142. using two primary antibodies for WB? - (reply: 3)
  143. reducing background in western... - ...by blocking the antibodies (reply: 2)
  144. Which bleed gives best result for antibody production? - (reply: 2)
  145. Using Western Antibodies for IHC - General rules- suggestions (reply: 3)
  146. Storage of antibodies - (reply: 3)
  147. Purified protein (his-tagged) multiple bands on gel - all picked up by antibody - what could they be? (reply: 2)
  148. Problem with Annexin FITC antibody - (reply: 5)
  149. Can antibody recommended for ELISA or WB be used on IHC? - (reply: 5)
  150. solubility and stability of monoclonal antibodies - (reply: 2)
  151. Standard Antibodies Against Separated Cellular Preps - (reply: 2)
  152. Anti-FLAG antibody working for Western blot - I unsucessfully tried many different (reply: 4)
  153. why some antibodies need overnight incubation for Western blot - (reply: 4)
  154. antibody inactivation of peptide - ELISA troubleshooting (reply: 1)
  155. Anti-PolyHistidin antibody - Best His6 antibody for western (reply: 2)
  156. Coupling Antibodies to alkaline phosphatase - (reply: 2)
  157. monoclonal antibody production - cell death in few wells (reply: 2)
  158. hybridoma cell line - (reply: 1)
  159. Will antibody need any buffer to make link with bead - Which buffer should I use to link bead with antibody (reply: 1)
  160. Monoclonal Ab is always from mice? - (reply: 3)
  161. Any experieces with unstable primary antibodies? - still recognizes recombinant protein but not native... HELP (reply: 1)
  162. two mouse antibodies can be stained with different secondaries? - different colors? (reply: 3)
  163. stability of hybridoma cell lines - how to produce totally stable hybridoma cel line? (reply: 3)
  164. One Antibody in Western Blot - (reply: 4)
  165. What epitope is my antibody recognizing? - (reply: 3)
  166. immunoprecipitation and western blotting assay by the same antibody - (reply: 4)
  167. no cross-reactivity at 81% homology using polyclonal antibodies? - (reply: 3)
  168. How to make an antibody against my protein - (reply: 4)
  169. Fab Antibody - (reply: 4)
  170. alpha2 gabaa antibody for mice please! - (reply: 1)
  171. Question on the recommended application of primary Antibody - (reply: 2)
  172. Purified antibodies in Western. - How can I find out how much to use? (reply: 12)
  173. Rabbit monoclonal antibody produce - (reply: 1)
  174. Amastigote of Leishmania-ELISA - Detaching of bound antibody (reply: 1)
  175. Creatine Kinase antibody problem. - (reply: 3)
  176. FACS analysis of receptor surface expression using fluorescent antibodies - (reply: 3)
  177. question about antibodies & protein G/A sepharose in CO-IP - (reply: 6)
  178. very strong secondary antibody background - (reply: 7)
  179. Antibody left overnight at room temperature - still usable? - (reply: 5)
  180. Big DNA contamination in antibody fraction, HELP ! - (reply: 1)
  181. antibody storage in skim milk - I want to know % of sodium azide (reply: 8)
  182. Reuse primary antibody - (reply: 4)
  183. How many antibody should be added for Immunoprecipitation - (reply: 7)
  184. Mouse Monoclonal Ki-67 ? - (reply: 1)
  185. antibody generation - big problem: PLEASE HELP (reply: 1)
  186. How to choose an antibody? - for western or other applications (reply: 1)
  187. confuse about which antibody to use as a first antibody - (reply: 2)
  188. proteins to raise the antibody againest to HIV - proteins (reply: 1)
  189. HELP: transfection reagent for suspension cell - mouse hybridoma siRNA transfection (reply: 2)
  190. Custom antibody advice (which company ?) - (reply: 2)
  191. Western Blots - Monoclonal or Polyclonal Antibody...? - Which one to use? (reply: 1)
  192. Background with chicken primary antibody - (reply: 2)
  193. Western blot background and specificity - How good is my antiserum? (reply: 1)
  194. antibody purification - (reply: 5)
  195. Antibody adsorption protocol - Immunology (reply: 1)
  196. 2 antibodies from same host - same host ab's in sandwich (reply: 4)
  197. antibody in SDS-containing solution - (reply: 3)
  198. cpti antibody - (reply: 3)
  199. purification of Anti-p24 antibody - purification of Anti-p24 antibody (reply: 1)
  200. On antibody storage and ChIP - (reply: 3)
  201. Self-purified antibody - viability and quantity problem - (reply: 1)
  202. Antibody Viability - are my antibodies alive?? (reply: 3)
  203. growing hybridomas - (reply: 1)
  204. Tissue staining problem with polyclonal antibody - (reply: 3)
  205. anyone has idea to raise antibody, how much protein need? - (reply: 1)
  206. antibody problems in western - Got similiar western signals for three different p (reply: 8)
  207. what is the role of glycine in antibody purificiation??? - (reply: 3)
  208. Antibody for inflammatory cells - (reply: 2)
  209. EPEC Antibody - Need commercial source of EPEC Antibody (reply: 1)
  210. Alkaline phosphatase cnjugated antibody - secondary antibody (reply: 5)
  211. Immunoprecipitation - getting rid of antibody heavy/light chains (reply: 6)
  212. Reliable vendor that sells small aliquots of Antibodies? - (reply: 1)
  213. Can antibodies and proteins be vortexed? - (reply: 4)
  214. what company make good antibody - (reply: 2)
  215. Help!! a-tubulin antibody did not work...... - (reply: 1)
  216. Monoclonal antibody but getting multiple bands??? - (reply: 3)
  217. anti-rodent secondary antibody - (reply: 2)
  218. hybridoma cell question - (reply: 4)
  219. Reuse antibodies for further incubation in Western blot - (reply: 5)
  220. antibodies to epitope tags - (reply: 1)
  221. Western blot detection other than antibody? - (reply: 5)
  222. Size of Antibodies - (reply: 1)
  223. monoclonal antibody production - monoclonal antibody production (reply: 3)
  224. phospho-specific antibody - (reply: 1)
  225. custom antibody synthesis - (reply: 1)
  226. phosphospecific antibody - how to get phosphospecific antibody (reply: 3)
  227. Antibodies: polyclonal vs monoclonal - (reply: 2)
  228. an anti-goat-HRP conjugate secondary antibody? - an anti-goat-HRP conjugate secondary antibody? (reply: 4)
  229. multiple bands from monoclonal antibody - (reply: 1)
  230. Western Blot after Coomassie stain of antibody? - Western Blotting after Coomassie stain? (reply: 3)
  231. Hybridoma stability - Hybridoma (reply: 19)
  232. Polyclonal vs. Monoclonal antibody for Sandwich ELISA? - (reply: 10)
  233. Antibodies using Recombinant proteins as antigen - (reply: 3)
  234. Antibodies to recombinant proteins - Any advice on production of proteins? (reply: 2)
  235. antigen / antibody immobilization - (reply: 1)
  236. SOD1 (Cu/Zn) ANTIBODY ? - (reply: 1)
  237. Murine MAb IgG purification - monoclonal antibody purification from ascites & supernatant (reply: 1)
  238. Cloning antibodies - (reply: 1)
  239. Hybridoma screening problem - (reply: 1)
  240. Insitu antibody staining - (reply: 1)
  241. Annti-Rad51/RecA antibodies - (reply: 1)
  242. how to reserve  antibody ofNOS? - (reply: 1)
  243. double label immunocytochemistry - protocol for antibodies from same animal (reply: 1)
  244. how to generate antibody? - (reply: 2)
  245. crosslinking of monoclonal Absto Protein G (or A) -Sepharose - (reply: 1)