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Antibody Related Discussions
Raising monoclonal antibodies - Monoclonal antibodies raised against similar sequence Antigens in mice
(reply: 2)
antibody titer -
(reply: 1)
which part of an antigen should monoclonal antibodies bind to? - how to choose appropiate epitopes?
(reply: 1)
Co-immunoprecipitation - Source of cheap irrelevant antibody
(reply: 2)
Antibody out of stock: ab8580 -
(reply: 4)
Secondary antibody -
(reply: 2)
antibody against His-tagged protein -
(reply: 3)
Visualizing CpG island in IF - Are there antibodies available?
(reply: 1)
mAB vs pAB -
(reply: 4)
Non-specific bands with secondary Antibody on Western Blotting -
(reply: 1)
Blocking antibody -
(reply: 1)
HSV1-Tk (mono/ployclonal) antibody. -
(reply: 1)
anyone knows good HA antibody for zebrafish whole mount stainging -
(reply: 4)
Antigen coated beads - For making antibodies
(reply: 1)
antibody to use for methylase chip - DNMT/MBD/MeCP2?
(reply: 3)
Immobilisation of Antibodies to Au ( Gold) surface -
(reply: 2)
antibodies working in indirect ELISA but not in inhibition ELISA -
(reply: 11)
Bacterial expression antibody recognition problem - antibodies won't recognize protein
(reply: 2)
Why do I get non-specific bands? - with my secondary antibody....
(reply: 5)
How to release antibodies from beads after IP? - in order to run ELISA after IP, not Western Blot.
(reply: 7)
Negative control for primary antibody in immunohistochemistry - What is it?
(reply: 9)
Coating with monoclonal/polyclonal -
(reply: 6)
Digestion of antibody using pepsin - no F(ab)2 was found
(reply: 1)
Control for primary antibody in Immunohistochemistry - What is it?
(reply: 4)
recombinant human antibody production problem -
(reply: 2)
How to reduce amount of antibodies used in WB -
(reply: 4)
Monoclonal vs Polyclonal Abs -
(reply: 1)
Resuspending lyophylized antibodies -
(reply: 1)
antibody storage - how to store?
(reply: 7)
Custom polyclonal peptide antibody services? -
(reply: 4)
gel and monoclonal antibody issues -
(reply: 3)
How to reuse antibody in Western blotting - Share your experience
(reply: 8)
Is my antibody specific? -
(reply: 3)
Can we reuse the primary antibody in immunohistochemistry? -
(reply: 3)
Testing the primary antibody - Is the secondary antibody recognizing the primary
(reply: 1)
Polyclonal antibody service - Who can you recommend?
(reply: 2)
Concentrating antibodies -
(reply: 3)
What is needed for a hybridoma growth culture -
(reply: 1)
Santa Cruz antibody -
(reply: 14)
Help: New to biochemistry and need help with antibody? -
(reply: 11)
affinity determination of an antibody -
(reply: 5)
Hybridoma cultures - floaters? -
(reply: 4)
polyclonal vs monoclonal -
(reply: 2)
Can I incubate membrane with 2 antibodies at the same time ? -
(reply: 5)
Western Blot Background - Having high backgrounds on western blotting due to antibody issues.
(reply: 2)
crosslinking antibodies before IP -
(reply: 2)
mouse anti-goat antibodies -
(reply: 1)
Monoclonal antibody affinity (Kd constant) determination using ELISA -
(reply: 2)
Can an antibody just stop working? -
(reply: 5)
HA antibody -
(reply: 1)
Diluting/mixing of antibodies - exact method? -
(reply: 1)
Blocking for phospsho antibodies -
(reply: 4)
Apoptosis detection with Phosphatidylserine antibody -
(reply: 4)
Frozen Antibody Lifespan -
(reply: 2)
anti-gfp antibodies -
(reply: 4)
Hybridoma culture not secreting mouse antibody -
(reply: 6)
mouse anti-mouse antibody ? -
(reply: 5)
Which p-Serine antibody is suitable for IP and WB -
(reply: 1)
"recombinanant antibody" -
(reply: 3)
How about the antibody quality of these two companies? - antibody quality
(reply: 5)
HELP! confusing antibody -
(reply: 14)
Denaturation of antibodies in high salt -
(reply: 3)
problem with anti-acetyl H3 antibody -
(reply: 11)
IP problems - The antibody doesn't bind to the beads
(reply: 5)
polyclonal antibodies go bad - when stored at -80????
(reply: 5)
shape-shifting antibodies? -
(reply: 11)
Making antibodies - Anybody has designed a custom antibody?
(reply: 4)
stripping antibodies in immunochemistry? -
(reply: 6)
antibody against yeast protein -
(reply: 1)
Antibodies for immunohistochemistry and cell ELISA - Are they the same?
(reply: 2)
antibody classification -
(reply: 1)
storage of ammonium sulphate purified antibodies -
(reply: 1)
Antibodies for different epitope giving bands of different sizes in the same sam -
(reply: 1)
Do antibody solutions settle? -
(reply: 2)
creating a neg. charge on an Antibodies' Fc region -
(reply: 2)
Why should some antibodies not be aliquoted? -
(reply: 12)
RNA polymerase II antibody recommendation? -
(reply: 2)
Mab purification - no mouse IgG band seen
(reply: 3)
Monoclonal screening of stable cells - How to maintain it to confluent stage?
(reply: 4)
macrophages collection for hybridoma -
(reply: 1)
Couple peptide to beads? for antibody purification -
(reply: 1)
Looking for an antibody/epitope type of search... -
(reply: 1)
polyclonal antibody production - is it necessay to remove fusion tag?
(reply: 2)
pSTAT5 antibody -
(reply: 5)
Ki67 Antibodies -
(reply: 6)
Antibody detecting albumin -
(reply: 1)
lyophilization of monoclonal antibodies... How? -
(reply: 5)
Affinity purification of antibody -
(reply: 2)
conformation specific monoclonal antibody - how to confirm?
(reply: 4)
antibody purification - how to get rid of bovine IgG?
(reply: 3)
Antibodies for ChiP - Of mice and men
(reply: 1)
polyclonal antibody production protocol - looking for protocols
(reply: 2)
monoclonal antibody production -
(reply: 6)
Human TERT Antibody -
(reply: 2)
humanizing an antibody? -
(reply: 5)
Polyclonal antibodies in immunocytochemistry not working -
(reply: 4)
Multiple antibodies in Western -
(reply: 5)
Are there antibodies to differentiate epithelial from fibroblasts cells? -
(reply: 5)
Rainbow MW marker from Amersham - detection with a mouse secondary antibody?
(reply: 1)
EMSA witj antibodies against TFII factors -
(reply: 3)
c-Myc antibody for Western -
(reply: 1)
Antibody concepts -
(reply: 20)
antibodies life - 4oC, -20oC or -80oC
(reply: 26)
Antibody produced in same species as target tissue - Will it cross react?
(reply: 2)
Western Blotting using Anti α Tubulin antibody: Bands too high! -
(reply: 3)
ChIP IgG has the same signal than the antibody tested -
(reply: 9)
NeuN and Ox-42 antibodies -
(reply: 4)
antibody against denatured protein? - basic questions
(reply: 2)
Immunofluorescence using polyclonal antibody -
(reply: 5)
monoclonal ab recognize one or more epitopes? -
(reply: 4)
Smearing antibody and DTT - how to interpret the gel? - Aggregated antibody
(reply: 1)
Antibody Mismatch Tolerance -
(reply: 2)
Problem in IP (too high background) - mAb shows a lot of backgroud+light chain.
(reply: 3)
estimation of concentration of Ab in hybridoma culture supernatant? -
(reply: 4)
can u guess the molecular weight of a raised antibody? -
(reply: 3)
dilution buffer for ap labeled 2nd antibody -
(reply: 1)
does 2nd antibody concentration matters? -
(reply: 3)
same proteins, same antibodies, different results -
(reply: 1)
Shipping antibodies -
(reply: 4)
Western blottting - Santacruz antibodies -
(reply: 10)
Can anyone recommend the best FLAG and c-myc antibodies for co-IP? -
(reply: 4)
Anybody has a good phosphorylated IKKa antibody? -
(reply: 3)
ASK1 antibody -
(reply: 2)
Antibody purification protocol --any suggestions? -
(reply: 12)
oligopeptide beased antibody for IP -
(reply: 2)
vortexing of antibodies upon dilution in 3% skim milk -
(reply: 3)
antibody concentration - antibody precipitation using ammonium sulfate
(reply: 2)
Concentration of antibody -
(reply: 5)
specific ser/Thr phosphorylation detection by in house antibody -
(reply: 1)
humanized antibody - how to design primers for CDRs - humanized antibody
(reply: 1)
Antibody question -
(reply: 5)
Polyclonal antibody purification? - how?
(reply: 9)
How can I elute antigen from proteinG+antibody+antigen complexes? -
(reply: 3)
questions about secondary antibody -
(reply: 2)
Rabbit IgG Isotype Control for Co-ip - Need available antibodies
(reply: 1)
Problem with antibodies -
(reply: 10)
antibody question -
(reply: 7)
can antibodies be added to cell culture for 10d before stain - immunocytochemistry
(reply: 1)
someone has frozen my antibody, is it still alive? -
(reply: 2)
Using denaturing protcol for his-protein purification - Can it still be used for antibody production?????
(reply: 4)
making a monoclonal from scratch... -
(reply: 1)
using two primary antibodies for WB? -
(reply: 3)
reducing background in western... - ...by blocking the antibodies
(reply: 2)
Which bleed gives best result for antibody production? -
(reply: 2)
Using Western Antibodies for IHC - General rules- suggestions
(reply: 3)
Storage of antibodies -
(reply: 3)
Purified protein (his-tagged) multiple bands on gel - all picked up by antibody - what could they be?
(reply: 2)
Problem with Annexin FITC antibody -
(reply: 5)
Can antibody recommended for ELISA or WB be used on IHC? -
(reply: 5)
solubility and stability of monoclonal antibodies -
(reply: 2)
Standard Antibodies Against Separated Cellular Preps -
(reply: 2)
Anti-FLAG antibody working for Western blot - I unsucessfully tried many different
(reply: 4)
why some antibodies need overnight incubation for Western blot -
(reply: 4)
antibody inactivation of peptide - ELISA troubleshooting
(reply: 1)
Anti-PolyHistidin antibody - Best His6 antibody for western
(reply: 2)
Coupling Antibodies to alkaline phosphatase -
(reply: 2)
monoclonal antibody production - cell death in few wells
(reply: 2)
hybridoma cell line -
(reply: 1)
Will antibody need any buffer to make link with bead - Which buffer should I use to link bead with antibody
(reply: 1)
Monoclonal Ab is always from mice? -
(reply: 3)
Any experieces with unstable primary antibodies? - still recognizes recombinant protein but not native... HELP
(reply: 1)
two mouse antibodies can be stained with different secondaries? - different colors?
(reply: 3)
stability of hybridoma cell lines - how to produce totally stable hybridoma cel line?
(reply: 3)
One Antibody in Western Blot -
(reply: 4)
What epitope is my antibody recognizing? -
(reply: 3)
immunoprecipitation and western blotting assay by the same antibody -
(reply: 4)
no cross-reactivity at 81% homology using polyclonal antibodies? -
(reply: 3)
How to make an antibody against my protein -
(reply: 4)
Fab Antibody -
(reply: 4)
alpha2 gabaa antibody for mice please! -
(reply: 1)
Question on the recommended application of primary Antibody -
(reply: 2)
Purified antibodies in Western. - How can I find out how much to use?
(reply: 12)
Rabbit monoclonal antibody produce -
(reply: 1)
Amastigote of Leishmania-ELISA - Detaching of bound antibody
(reply: 1)
Creatine Kinase antibody problem. -
(reply: 3)
FACS analysis of receptor surface expression using fluorescent antibodies -
(reply: 3)
question about antibodies & protein G/A sepharose in CO-IP -
(reply: 6)
very strong secondary antibody background -
(reply: 7)
Antibody left overnight at room temperature - still usable? -
(reply: 5)
Big DNA contamination in antibody fraction, HELP ! -
(reply: 1)
antibody storage in skim milk - I want to know % of sodium azide
(reply: 8)
Reuse primary antibody -
(reply: 4)
How many antibody should be added for Immunoprecipitation -
(reply: 7)
Mouse Monoclonal Ki-67 ? -
(reply: 1)
antibody generation - big problem: PLEASE HELP
(reply: 1)
How to choose an antibody? - for western or other applications
(reply: 1)
confuse about which antibody to use as a first antibody -
(reply: 2)
proteins to raise the antibody againest to HIV - proteins
(reply: 1)
HELP: transfection reagent for suspension cell - mouse hybridoma siRNA transfection
(reply: 2)
Custom antibody advice (which company ?) -
(reply: 2)
Western Blots - Monoclonal or Polyclonal Antibody...? - Which one to use?
(reply: 1)
Background with chicken primary antibody -
(reply: 2)
Western blot background and specificity - How good is my antiserum?
(reply: 1)
antibody purification -
(reply: 5)
Antibody adsorption protocol - Immunology
(reply: 1)
2 antibodies from same host - same host ab's in sandwich
(reply: 4)
antibody in SDS-containing solution -
(reply: 3)
cpti antibody -
(reply: 3)
purification of Anti-p24 antibody - purification of Anti-p24 antibody
(reply: 1)
On antibody storage and ChIP -
(reply: 3)
Self-purified antibody - viability and quantity problem -
(reply: 1)
Antibody Viability - are my antibodies alive??
(reply: 3)
growing hybridomas -
(reply: 1)
Tissue staining problem with polyclonal antibody -
(reply: 3)
anyone has idea to raise antibody, how much protein need? -
(reply: 1)
antibody problems in western - Got similiar western signals for three different p
(reply: 8)
what is the role of glycine in antibody purificiation??? -
(reply: 3)
Antibody for inflammatory cells -
(reply: 2)
EPEC Antibody - Need commercial source of EPEC Antibody
(reply: 1)
Alkaline phosphatase cnjugated antibody - secondary antibody
(reply: 5)
Immunoprecipitation - getting rid of antibody heavy/light chains
(reply: 6)
Reliable vendor that sells small aliquots of Antibodies? -
(reply: 1)
Can antibodies and proteins be vortexed? -
(reply: 4)
what company make good antibody -
(reply: 2)
Help!! a-tubulin antibody did not work...... -
(reply: 1)
Monoclonal antibody but getting multiple bands??? -
(reply: 3)
anti-rodent secondary antibody -
(reply: 2)
hybridoma cell question -
(reply: 4)
Reuse antibodies for further incubation in Western blot -
(reply: 5)
antibodies to epitope tags -
(reply: 1)
Western blot detection other than antibody? -
(reply: 5)
Size of Antibodies -
(reply: 1)
monoclonal antibody production - monoclonal antibody production
(reply: 3)
phospho-specific antibody -
(reply: 1)
custom antibody synthesis -
(reply: 1)
phosphospecific antibody - how to get phosphospecific antibody
(reply: 3)
Antibodies: polyclonal vs monoclonal -
(reply: 2)
an anti-goat-HRP conjugate secondary antibody? - an anti-goat-HRP conjugate secondary antibody?
(reply: 4)
multiple bands from monoclonal antibody -
(reply: 1)
Western Blot after Coomassie stain of antibody? - Western Blotting after Coomassie stain?
(reply: 3)
Hybridoma stability - Hybridoma
(reply: 19)
Polyclonal vs. Monoclonal antibody for Sandwich ELISA? -
(reply: 10)
Antibodies using Recombinant proteins as antigen -
(reply: 3)
Antibodies to recombinant proteins - Any advice on production of proteins?
(reply: 2)
antigen / antibody immobilization -
(reply: 1)
SOD1 (Cu/Zn) ANTIBODY ? -
(reply: 1)
Murine MAb IgG purification - monoclonal antibody purification from ascites & supernatant
(reply: 1)
Cloning antibodies -
(reply: 1)
Hybridoma screening problem -
(reply: 1)
Insitu antibody staining -
(reply: 1)
Annti-Rad51/RecA antibodies -
(reply: 1)
how to reserve antibody ofNOS? -
(reply: 1)
double label immunocytochemistry - protocol for antibodies from same animal
(reply: 1)
how to generate antibody? -
(reply: 2)
crosslinking of monoclonal Absto Protein G (or A) -Sepharose -
(reply: 1)