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Antibody Related Discussions
  1. Can we reuse the primary antibody in immunohistochemistry? - (reply: 3)
  2. Testing the primary antibody - Is the secondary antibody recognizing the primary (reply: 1)
  3. monoclonal antibody or polyclonal antibody? - which is better for ChIP? (reply: 1)
  4. Polyclonal antibody service - Who can you recommend? (reply: 2)
  5. what does "(H + L)" mean in the secondary antibody? - (reply: 3)
  6. Concentrating antibodies - (reply: 3)
  7. KLH-based immunization protocols: suitable for hybridoma production purposes? - (reply: 4)
  8. What is needed for a hybridoma growth culture - (reply: 1)
  9. How dilute an antibody 1:150000 - (reply: 2)
  10. Santa Cruz antibody - (reply: 11)
  11. WB antibody to be used in IHC - (reply: 7)
  12. Help: New to biochemistry and need help with antibody? - (reply: 11)
  13. mouse and rabbit antibodies in western blot - (reply: 2)
  14. affinity determination of an antibody - (reply: 5)
  15. How to dilute an antibody? - (reply: 2)
  16. Hybridoma cultures - floaters? - (reply: 4)
  17. how to label an antibody - (reply: 3)
  18. polyclonal vs monoclonal - (reply: 2)
  19. Help choosing an anti-human antibody - (reply: 2)
  20. Can I incubate membrane with 2 antibodies at the same time ? - (reply: 5)
  21. Specifity of antibodies - (reply: 2)
  22. Western Blot Background - Having high backgrounds on western blotting due to antibody issues. (reply: 2)
  23. which conjugate for the secondary antibody in immunohistochemistry - (reply: 1)
  24. crosslinking antibodies before IP - (reply: 2)
  25. ELISA Problem since change of antibody supplier? - (reply: 1)
  26. mouse anti-goat antibodies - (reply: 1)
  27. antibody gold labeling - (reply: 3)
  28. Monoclonal antibody affinity (Kd constant) determination using ELISA - (reply: 2)
  29. Antigen Anti body and Protein - (reply: 3)
  30. Can an antibody just stop working? - (reply: 5)
  31. Decrease in serum antibody titers - (reply: 3)
  32. HA antibody - (reply: 1)
  33. Antibody help please - (reply: 3)
  34. Diluting/mixing of antibodies - exact method? - (reply: 1)
  35. Opinion about fluorescent antibodies - (reply: 6)
  36. Blocking for phospsho antibodies - (reply: 4)
  37. secondary antibody labeling with NHS ester functionalized help? - (reply: 1)
  38. Apoptosis detection with Phosphatidylserine antibody - (reply: 4)
  39. Qiagen penta-HIS antibody not reusable - looses activity after freeze-thaw-cycles (reply: 2)
  40. Frozen Antibody Lifespan - (reply: 2)
  41. polyclonal antibodies using inclusion bodies - how to prepare sample (reply: 1)
  42. anti-gfp antibodies - (reply: 4)
  43. labelling primary antibody with fluorescent tag - (reply: 1)
  44. Hybridoma culture not secreting mouse antibody - (reply: 6)
  45. How much of antibody to add? - (reply: 3)
  46. mouse anti-mouse antibody ? - (reply: 5)
  47. ELISA - coating antibody (reply: 3)
  48. Which p-Serine antibody is suitable for IP and WB - (reply: 1)
  49. looking for TNF alpha antibody for human tissue IHC - (reply: 1)
  50. "recombinanant antibody" - (reply: 3)
  51. production of monoclonal antibody when the host protein is having sequence simil - (reply: 3)
  52. How about the antibody quality of these two companies? - antibody quality (reply: 5)
  53. secondary antibody? - (reply: 4)
  54. HELP! confusing antibody - (reply: 14)
  55. preferred method for purifying antibodies - (reply: 2)
  56. Denaturation of antibodies in high salt - (reply: 3)
  57. antibodies testing - (reply: 4)
  58. problem with anti-acetyl H3 antibody - (reply: 11)
  59. how to select the proper antibody - (reply: 5)
  60. IP problems - The antibody doesn't bind to the beads (reply: 5)
  61. fluorescent secondary antibodies --> reccomendations? - (reply: 6)
  62. polyclonal antibodies go bad - when stored at -80???? (reply: 5)
  63. antibody incubation on western blots - difference between 4°C and RT (reply: 10)
  64. shape-shifting antibodies? - (reply: 11)
  65. My antibody does not work for IP, but works well fpr western? - antibofy not working for IP (reply: 12)
  66. Making antibodies - Anybody has designed a custom antibody? (reply: 4)
  67. What are the differences between monocloan and polyclonal antibody? - help! (reply: 1)
  68. stripping antibodies in immunochemistry? - (reply: 6)
  69. Affinity Purified Antibody - (reply: 1)
  70. antibody against yeast protein - (reply: 1)
  71. reusing PVDF membrane for different antibodies without stripping - (reply: 5)
  72. Antibodies for immunohistochemistry and cell ELISA - Are they the same? (reply: 2)
  73. purification of HRP antibody conjugate - (reply: 10)
  74. antibody classification - (reply: 1)
  75. Freezed antibodies - (reply: 2)
  76. storage of ammonium sulphate purified antibodies - (reply: 1)
  77. native or denatured protein? - for antibodies (reply: 5)
  78. Antibodies for different epitope giving bands of different sizes in the same sam - (reply: 1)
  79. Antibodies to stain vesicles/vacuoles in my cells? - (reply: 4)
  80. Do antibody solutions settle? - (reply: 2)
  81. antibody labelling for immunochromatographic assay - (reply: 4)
  82. creating a neg. charge on an Antibodies' Fc region - (reply: 2)
  83. Looking for primary antibody to BiP (grp78) for flow cytometry - (reply: 2)
  84. Why should some antibodies not be aliquoted? - (reply: 12)
  85. Primary-Secondary Antibody Question - (reply: 5)
  86. RNA polymerase II antibody recommendation? - (reply: 2)
  87. protein estimation of monoclonal antibody - inconsistent values (reply: 2)
  88. Mab purification - no mouse IgG band seen (reply: 3)
  89. Confirming IP with WB - Can I use the same antibody for both IP and WB? (reply: 5)
  90. Monoclonal screening of stable cells - How to maintain it to confluent stage? (reply: 4)
  91. Antibody not binding to protein A/G - Immunoprecipitation (reply: 3)
  92. macrophages collection for hybridoma - (reply: 1)
  93. Is it OK to use monoclonal antibody against its host protein for westen - (reply: 4)
  94. Couple peptide to beads? for antibody purification - (reply: 1)
  95. Did my Ab provider do me wrong? - Judging the purity of my antibody (reply: 3)
  96. Looking for an antibody/epitope type of search... - (reply: 1)
  97. Could protein modification block an antibody epitope? - (reply: 4)
  98. polyclonal antibody production - is it necessay to remove fusion tag? (reply: 2)
  99. Antibody for EMSA - (reply: 2)
  100. pSTAT5 antibody - (reply: 5)
  101. Is my secondary antibody the cause for non-specific signal? - (reply: 3)
  102. Ki67 Antibodies - (reply: 6)
  103. Anti-hunman CD monoclonal antibody - (reply: 1)
  104. Antibody detecting albumin - (reply: 1)
  105. anti-flag antibodies - (reply: 6)
  106. lyophilization of monoclonal antibodies... How? - (reply: 5)
  107. Antibody storage - (reply: 4)
  108. Affinity purification of antibody - (reply: 2)
  109. Antibodies and triton X - (reply: 4)
  110. conformation specific monoclonal antibody - how to confirm? (reply: 4)
  111. Problem with Monoclonal antibody production - why cells die after adding HAT?????? (reply: 6)
  112. antibody purification - how to get rid of bovine IgG? (reply: 3)
  113. Stability of primary antibodies at room temperature - (reply: 4)
  114. Antibodies for ChiP - Of mice and men (reply: 1)
  115. Oligodendrocyte's and tubulin antibody in Western Blot - (reply: 1)
  116. polyclonal antibody production protocol - looking for protocols (reply: 2)
  117. Good GFP antibody from SANTA CRUZ for Western Blotting? - (reply: 7)
  118. monoclonal antibody production - (reply: 6)
  119. Catalase Antibody - (reply: 2)
  120. Human TERT Antibody - (reply: 2)
  121. Need clean HAtag antibody for mouse tissue lysates - (reply: 1)
  122. humanizing an antibody? - (reply: 5)
  123. Catalase Antibody - (reply: 1)
  124. Polyclonal antibodies in immunocytochemistry not working - (reply: 4)
  125. FLAG antibodies for mouse samples - Immunofluorescence/Flow cytometry (reply: 1)
  126. Multiple antibodies in Western - (reply: 5)
  127. ELISA/ BSA in capture antibody storage buffer - (reply: 1)
  128. Are there antibodies to differentiate epithelial from fibroblasts cells? - (reply: 5)
  129. need antibody in TX - (reply: 1)
  130. Rainbow MW marker from Amersham - detection with a mouse secondary antibody? (reply: 1)
  131. using IgM antibodies in IP - (reply: 3)
  132. EMSA witj antibodies against TFII factors - (reply: 3)
  133. Monoclonal antibodies production older mice - (reply: 1)
  134. c-Myc antibody for Western - (reply: 1)
  135. Working antibody for human NFkB (p65) ChIP assay ? - (reply: 2)
  136. Antibody concepts - (reply: 20)
  137. Stable cell line, polyclonal and monoclonal. - Which is better? (reply: 4)
  138. antibodies life - 4oC, -20oC or -80oC (reply: 25)
  139. How to calculate antibody concentration from OD280 readings? - (reply: 2)
  140. Antibody produced in same species as target tissue - Will it cross react? (reply: 2)
  141. Does this antibody work? - (reply: 3)
  142. Western Blotting using Anti α Tubulin antibody: Bands too high! - (reply: 3)
  143. Antibody stability and additives - (reply: 4)
  144. ChIP IgG has the same signal than the antibody tested - (reply: 9)
  145. Best Chromatin IP antibody?(tag) - (reply: 3)
  146. NeuN and Ox-42 antibodies - (reply: 4)
  147. how to check protein-primary antibody binding - Is there any method or technique to check or proof read the primary Ab (reply: 7)
  148. antibody against denatured protein? - basic questions (reply: 2)
  149. Disaster! - Wrong temperature for antibody (reply: 9)
  150. Immunofluorescence using polyclonal antibody - (reply: 5)
  151. Weak and variable signal blotting for MDR1 using C219 monoclonal Ab - (reply: 2)
  152. monoclonal ab recognize one or more epitopes? - (reply: 4)
  153. Co-immunoprecipitation - antigen elution without the antibody - (reply: 4)
  154. Smearing antibody and DTT - how to interpret the gel? - Aggregated antibody (reply: 1)
  155. secondary antibody - (reply: 2)
  156. Antibody Mismatch Tolerance - (reply: 2)
  157. Storage of diluted antibodies - (reply: 6)
  158. Problem in IP (too high background) - mAb shows a lot of backgroud+light chain. (reply: 3)
  159. Antibody crossreactivity - (reply: 1)
  160. estimation of concentration of Ab in hybridoma culture supernatant? - (reply: 4)
  161. Antibody treatment - (reply: 2)
  162. can u guess the molecular weight of a raised antibody? - (reply: 3)
  163. Antibody storage in 5% milk - (reply: 1)
  164. dilution buffer for ap labeled 2nd antibody - (reply: 1)
  165. antibody cocktails - (reply: 4)
  166. does 2nd antibody concentration matters? - (reply: 3)
  167. Phage Display: Not getting antibodies after 3 rounds of panning - (reply: 6)
  168. same proteins, same antibodies, different results - (reply: 1)
  169. antibodies from hybridoma - (reply: 6)
  170. Shipping antibodies - (reply: 4)
  171. Freeze-thaw effects on antibodies and samples - (reply: 1)
  172. Western blottting - Santacruz antibodies - (reply: 10)
  173. Double staining antibody plus Annexin! - Help (reply: 2)
  174. Can anyone recommend the best FLAG and c-myc antibodies for co-IP? - (reply: 4)
  175. A great case problem on antibody assay - (reply: 2)
  176. Anybody has a good phosphorylated IKKa antibody? - (reply: 3)
  177. Antibody Dilution - (reply: 10)
  178. ASK1 antibody - (reply: 2)
  179. What is the sensitivity of dot blot compared to ELISA, and what antibody concent - (reply: 1)
  180. Antibody purification protocol --any suggestions? - (reply: 12)
  181. hrp labeling of antibodies - (reply: 3)
  182. oligopeptide beased antibody for IP - (reply: 2)
  183. NGF deprivation: why anti-NGF antibody? - (reply: 3)
  184. vortexing of antibodies upon dilution in 3% skim milk - (reply: 3)
  185. My antibody is forming a precipitate! - Why? (reply: 7)
  186. antibody concentration - antibody precipitation using ammonium sulfate (reply: 2)
  187. question on NF-kB antibody - (reply: 2)
  188. Concentration of antibody - (reply: 5)
  189. antibody - (reply: 4)
  190. specific ser/Thr phosphorylation detection by in house antibody - (reply: 1)
  191. Selection of Antibodies for CHip - (reply: 7)
  192. humanized antibody - how to design primers for CDRs - humanized antibody (reply: 1)
  193. help mAb - monocloanal antibodies (reply: 2)
  194. Antibody question - (reply: 5)
  195. Removing LPS from mAb stocks - (reply: 1)
  196. Polyclonal antibody purification? - how? (reply: 9)
  197. Multiple Bands in Westren Blot? - My antibodies are polyclonal...is dat d reason im getting multiple bands? (reply: 2)
  198. How can I elute antigen from proteinG+antibody+antigen complexes? - (reply: 3)
  199. antibody purification - how to purify from serum free medium (reply: 1)
  200. questions about secondary antibody - (reply: 2)
  201. Antibody from short peptides - Time saving, but do they work for both West and ELISAs (reply: 4)
  202. Rabbit IgG Isotype Control for Co-ip - Need available antibodies (reply: 1)
  203. Basic questions.. - antibody titer (reply: 5)
  204. Problem with antibodies - (reply: 10)
  205. detecting transmembrane protein exoression for monoclonal ab production - (reply: 1)
  206. antibody question - (reply: 7)
  207. spleenocyte count for hybridoma production - (reply: 1)
  208. can antibodies be added to cell culture for 10d before stain - immunocytochemistry (reply: 1)
  209. What can I do with blood sample from immunized rabbit? - we want to know whether there's antibody or not. (reply: 5)
  210. someone has frozen my antibody, is it still alive? - (reply: 2)
  211. Co-IP with two polyclonal antibodies - (reply: 2)
  212. Using denaturing protcol for his-protein purification - Can it still be used for antibody production????? (reply: 4)
  213. Secondary Antibody Choice - (reply: 6)
  214. making a monoclonal from scratch... - (reply: 1)
  215. which company can raise good antibodies? - raising antibodies using the native protein (reply: 3)
  216. using two primary antibodies for WB? - (reply: 3)
  217. Western Blot - unspecific antibody binding - (reply: 9)
  218. reducing background in western... - ...by blocking the antibodies (reply: 2)
  219. Secondary antibody - need to strip blot? - (reply: 4)
  220. Which bleed gives best result for antibody production? - (reply: 2)
  221. primary antibody for neuron - (reply: 6)
  222. Using Western Antibodies for IHC - General rules- suggestions (reply: 3)
  223. What kind of antibodies to use? Conjugated vs. unconjugated? - (reply: 19)
  224. Storage of antibodies - (reply: 3)
  225. antibody array for histone modification identification - (reply: 2)
  226. Purified protein (his-tagged) multiple bands on gel - all picked up by antibody - what could they be? (reply: 2)
  227. Detecting phosphorylated PKR - antibody and positive control - (reply: 4)
  228. Problem with Annexin FITC antibody - (reply: 5)
  229. Sandwich ELISA antibodies - (reply: 3)
  230. Can antibody recommended for ELISA or WB be used on IHC? - (reply: 5)
  231. Does HIV antibody kill HIV? - (reply: 6)
  232. solubility and stability of monoclonal antibodies - (reply: 2)
  233. Selecting epitomes of antibodies to apply in Western Blotting - (reply: 1)
  234. Standard Antibodies Against Separated Cellular Preps - (reply: 2)
  235. Array antibodies onto membranes - (reply: 4)
  236. Anti-FLAG antibody working for Western blot - I unsucessfully tried many different (reply: 4)
  237. Two different antibodies necessary for sandwich ELISA? - (reply: 3)
  238. why some antibodies need overnight incubation for Western blot - (reply: 4)
  239. aminocoumarin immunoprecipitation - looking for primary antibody (reply: 1)
  240. antibody inactivation of peptide - ELISA troubleshooting (reply: 1)
  241. Monoclonal antibody production - no cells show high O.D. - (reply: 8)
  242. Anti-PolyHistidin antibody - Best His6 antibody for western (reply: 2)
  243. Antibody for Sialic Acid residues - (reply: 3)
  244. Coupling Antibodies to alkaline phosphatase - (reply: 2)
  245. monoclonal antibody - use of HT (reply: 2)
  246. monoclonal antibody production - cell death in few wells (reply: 2)
  247. Polyclonal Ab versus Monoclonal Ab for immunoaffinity chromatography - (reply: 1)
  248. hybridoma cell line - (reply: 1)
  249. Western Blotting detection Antibodies........... - Are anti-sheep and anti-goat HRP interchangable? (reply: 2)
  250. Will antibody need any buffer to make link with bead - Which buffer should I use to link bead with antibody (reply: 1)
  251. Bacterial antibodies as the controls for Western - (reply: 1)
  252. Monoclonal Ab is always from mice? - (reply: 3)
  253. Antibodies to estrogen receptor, recommendations? - (reply: 1)
  254. Any experieces with unstable primary antibodies? - still recognizes recombinant protein but not native... HELP (reply: 1)
  255. recommendation on mitochondria matrix protein antibody? - (reply: 1)
  256. two mouse antibodies can be stained with different secondaries? - different colors? (reply: 3)
  257. PARP antibody working condition - (reply: 3)
  258. stability of hybridoma cell lines - how to produce totally stable hybridoma cel line? (reply: 3)
  259. Who sells good primary antibodies for Westerns? - Who's your favourite supplier? Least favourite? (reply: 24)
  260. One Antibody in Western Blot - (reply: 4)
  261. how can i preserve the purified mAb for a long time? - (reply: 7)
  262. What epitope is my antibody recognizing? - (reply: 3)
  263. Antibody Position on ELISA plate - (reply: 7)
  264. immunoprecipitation and western blotting assay by the same antibody - (reply: 4)
  265. Problems with protein expression - Protein expression, Antibody production (reply: 2)
  266. no cross-reactivity at 81% homology using polyclonal antibodies? - (reply: 3)
  267. polyclonal antibody doesn't pick up my protein during western!? - (reply: 4)
  268. How to make an antibody against my protein - (reply: 4)
  269. Antibody Vs. silver - (reply: 1)
  270. Fab Antibody - (reply: 4)
  271. Antibody source? - canine antibodies (reply: 1)
  272. alpha2 gabaa antibody for mice please! - (reply: 1)
  273. how many antibody eveyone use for CHIP assay - (reply: 2)
  274. Question on the recommended application of primary Antibody - (reply: 2)
  275. Co-IP experiments, Ratio of amount of cells/lysis volume/ug antibodies - (reply: 2)
  276. Purified antibodies in Western. - How can I find out how much to use? (reply: 12)
  277. Immunoaffinity chromatography-help - protocol for coupling of antibodies (reply: 2)
  278. Rabbit monoclonal antibody produce - (reply: 1)
  279. Caco-2 Vs Hybridoma cells - contamination of Caco-2 cell line with hybridoma cell line (reply: 3)
  280. Amastigote of Leishmania-ELISA - Detaching of bound antibody (reply: 1)
  281. How much Antibody did I get? - Protein G column (reply: 1)
  282. Creatine Kinase antibody problem. - (reply: 3)
  283. Nuclear staining - staining with a non-nuclear antibody - (reply: 1)
  284. FACS analysis of receptor surface expression using fluorescent antibodies - (reply: 3)
  285. western blot secondary antibody incubation time - will long incubation cause high background? (reply: 3)
  286. question about antibodies & protein G/A sepharose in CO-IP - (reply: 6)
  287. Low PCR product for antibody gene - (reply: 4)
  288. very strong secondary antibody background - (reply: 7)
  289. Choice of colour of secondary antibody for IHC - (reply: 2)
  290. Antibody left overnight at room temperature - still usable? - (reply: 5)
  291. Two antibodies on one western Blot? - (reply: 4)
  292. Big DNA contamination in antibody fraction, HELP ! - (reply: 1)
  293. Creative ways to get my hands antibodies - ...for free (reply: 35)
  294. antibody storage in skim milk - I want to know % of sodium azide (reply: 8)
  295. Antibodies of different class in blood of different species. - (reply: 3)
  296. Reuse primary antibody - (reply: 4)
  297. non IgG secreting hybridomas - (reply: 7)
  298. How many antibody should be added for Immunoprecipitation - (reply: 7)
  299. Antibody with sodiem azide added to mammalian cells - (reply: 2)
  300. Mouse Monoclonal Ki-67 ? - (reply: 1)
  301. Getting my primary antibody for Western - (reply: 6)
  302. antibody generation - big problem: PLEASE HELP (reply: 1)
  303. antibody production - animal free systems? (reply: 3)
  304. How to choose an antibody? - for western or other applications (reply: 1)
  305. high antibody binding non specifically - (reply: 3)
  306. confuse about which antibody to use as a first antibody - (reply: 2)
  307. Non-specific binding of secondary antibodies in IF - Secondaries stain Swiss 3T3 nucleoli (reply: 1)
  308. proteins to raise the antibody againest to HIV - proteins (reply: 1)
  309. Role of buffer in antibody purification - (reply: 3)
  310. HELP: transfection reagent for suspension cell - mouse hybridoma siRNA transfection (reply: 2)
  311. How to conjugate the antibody to the beads. - (reply: 4)
  312. Custom antibody advice (which company ?) - (reply: 2)
  313. Same antibody for immunohistochemistry and immunocytochemistry - SAME ANTIBODY FOR IHC AND ICC (reply: 1)
  314. Western Blots - Monoclonal or Polyclonal Antibody...? - Which one to use? (reply: 1)
  315. Antibody - (reply: 1)
  316. Background with chicken primary antibody - (reply: 2)
  317. Protein Purification - Advice - dialysis/desalting/concnetrating and antibody production? (reply: 1)
  318. Western blot background and specificity - How good is my antiserum? (reply: 1)
  319. ...the best antibodies? - (reply: 10)
  320. antibody purification - (reply: 5)
  321. Anti-His tag monoclonal antibody - (reply: 3)
  322. Antibody adsorption protocol - Immunology (reply: 1)
  323. conservation of antibodies! - after purification! (reply: 5)
  324. 2 antibodies from same host - same host ab's in sandwich (reply: 4)
  325. Hybridoma Cell lines - How to do it (reply: 1)
  326. antibody in SDS-containing solution - (reply: 3)
  327. Dnmt1 antibody - (reply: 1)
  328. cpti antibody - (reply: 3)
  329. p85 antibody from Santa Cruz - (reply: 6)
  330. purification of Anti-p24 antibody - purification of Anti-p24 antibody (reply: 1)
  331. Antibody testing - what if antigen is not available in a soluble form? (reply: 2)
  332. On antibody storage and ChIP - (reply: 3)
  333. Help with purification of antibody against a GST-fusion - Antibody purification (reply: 2)
  334. Self-purified antibody - viability and quantity problem - (reply: 1)
  335. Incubating the filters two overnights with primary antibody. - WESTERN BLOTTING (reply: 3)
  336. Antibody Viability - are my antibodies alive?? (reply: 3)
  337. How are antibodies typically injected into living systems? - (reply: 6)
  338. growing hybridomas - (reply: 1)
  339. CS antibodies - (reply: 5)
  340. Tissue staining problem with polyclonal antibody - (reply: 3)
  341. Looking for ELISA kit for proteinase 3 - Not anti-proteinase 3 antibody ELISA kit (reply: 1)
  342. anyone has idea to raise antibody, how much protein need? - (reply: 1)
  343. why did precipitate formed after antibody freez/thaw ? - (reply: 2)
  344. antibody problems in western - Got similiar western signals for three different p (reply: 8)
  345. Freezing method for Hybridoma - (reply: 1)
  346. what is the role of glycine in antibody purificiation??? - (reply: 3)
  347. Which antibody to use for ChiP? - Monoclonal or polyclonal? (reply: 5)
  348. Antibody for inflammatory cells - (reply: 2)
  349. Source for PAR antibody? - IHC for Poly-ADP-Ribose on rat tissues (reply: 2)
  350. EPEC Antibody - Need commercial source of EPEC Antibody (reply: 1)
  351. My antibody recognizes two bands - I tried everything and I have still two bands (reply: 2)
  352. Alkaline phosphatase cnjugated antibody - secondary antibody (reply: 5)
  353. antibody clones? - (reply: 2)
  354. Immunoprecipitation - getting rid of antibody heavy/light chains (reply: 6)
  355. Antibody Substitutes - (reply: 1)
  356. Reliable vendor that sells small aliquots of Antibodies? - (reply: 1)
  357. Immunoprecipitation - removing antibody? (reply: 4)
  358. Can antibodies and proteins be vortexed? - (reply: 4)
  359. Recycling antibodies in confocal microscopy?? - (reply: 2)
  360. what company make good antibody - (reply: 2)
  361. How many times do you reuse a primary antibody? - (reply: 7)
  362. Help!! a-tubulin antibody did not work...... - (reply: 1)
  363. Difference between polyclonal and monoclonal anitbodies - (reply: 2)
  364. Monoclonal antibody but getting multiple bands??? - (reply: 3)
  365. Help on ELISA antibody binding principles - (reply: 2)
  366. anti-rodent secondary antibody - (reply: 2)
  367. phospho-(Ser) PKC substrate antibody - (reply: 1)
  368. hybridoma cell question - (reply: 4)
  369. Need some antibodies - (reply: 2)
  370. Reuse antibodies for further incubation in Western blot - (reply: 5)
  371. phospho antibody detect untreat lane - (reply: 6)
  372. antibodies to epitope tags - (reply: 1)
  373. p16 antibody in western blotting, weird results - western blotting, p16 (reply: 4)
  374. Western blot detection other than antibody? - (reply: 5)
  375. Phospho-Elk1 specific Antibody...which band?? - (reply: 2)
  376. Size of Antibodies - (reply: 1)
  377. Multiplex Cytokine Assays - Antibody coupling - (reply: 3)
  378. monoclonal antibody production - monoclonal antibody production (reply: 3)
  379. online database for antibodies - (reply: 3)
  380. phospho-specific antibody - (reply: 1)
  381. Immunoprecipitation - peptide antibody (reply: 2)
  382. custom antibody synthesis - (reply: 1)
  383. anti mouse BCRP1 - polyclonal antibodies (reply: 1)
  384. phosphospecific antibody - how to get phosphospecific antibody (reply: 3)
  385. production of monoclonal antibodies - (reply: 2)
  386. Antibodies: polyclonal vs monoclonal - (reply: 2)
  387. western blotting with anti-goat HRP - bands after ECL but only secondary antibody (reply: 1)
  388. an anti-goat-HRP conjugate secondary antibody? - an anti-goat-HRP conjugate secondary antibody? (reply: 4)
  389. antibodies against membrane proteins - detection failure (reply: 3)
  390. multiple bands from monoclonal antibody - (reply: 1)
  391. antibodies against methyldeoxycytidine? - (reply: 1)
  392. Western Blot after Coomassie stain of antibody? - Western Blotting after Coomassie stain? (reply: 3)
  393. antibody shelf life? - (reply: 2)
  394. Hybridoma stability - Hybridoma (reply: 19)
  395. antibody to EGFP - anti-EGFP (reply: 6)
  396. Polyclonal vs. Monoclonal antibody for Sandwich ELISA? - (reply: 10)
  397. SDS PAGE - Validate method for MAb purity test (reply: 3)
  398. Antibodies using Recombinant proteins as antigen - (reply: 3)
  399. antibody stability - (reply: 2)
  400. Antibodies to recombinant proteins - Any advice on production of proteins? (reply: 2)
  401. Antibodies to Recombinant Proteins - Advice on antibody production?? (reply: 1)
  402. antigen / antibody immobilization - (reply: 1)
  403. how to do the antibody supershift through PAGE gel - (reply: 2)
  404. SOD1 (Cu/Zn) ANTIBODY ? - (reply: 1)
  405. Antibody against XanthineOxidase - (reply: 1)
  406. Murine MAb IgG purification - monoclonal antibody purification from ascites & supernatant (reply: 1)
  407. Monoclonal antibodies on MOUSE Tissue - Monoclonal antibodies on MOUSE Tissue (reply: 2)
  408. Cloning antibodies - (reply: 1)
  409. von Willebrands primary antibody - (reply: 1)
  410. Hybridoma screening problem - (reply: 1)
  411. hybridomona production - (reply: 2)
  412. Insitu antibody staining - (reply: 1)
  413. MHC Class I Western blotantibody??? - (reply: 1)
  414. Annti-Rad51/RecA antibodies - (reply: 1)
  415. labeling antibody withfluorescein - (reply: 1)
  416. how to reserve  antibody ofNOS? - (reply: 1)
  417. ELISA incubator - need an incubator or oven for capturing antibody (reply: 1)
  418. double label immunocytochemistry - protocol for antibodies from same animal (reply: 1)
  419. how to generate antibody? - (reply: 2)
  420. His-tag Westerns: Bestcommercial antibody - (reply: 1)
  421. crosslinking of monoclonal Absto Protein G (or A) -Sepharose - (reply: 1)