hybridomona production - (Oct/04/2001 )

Hi, RajeshPlease, take your Balb/c mouse and injected into its abdomen cavity of 0.5ml pristane (Sigma) after this of 1 to 2 week reinjected by similar route 1*10(6) your hybridoma cells and to 14-21 days aspirated the ascitic fluid containing essential mAbs.This is all.Best Research to YouDmitriy

hai ,can some one give me a protocol for hybridomona production in peritonium in mice.please send me soonthank yourajesh

Hi Dimitriy,I usally produce hybridoma in vitro. Could you give me more detail the method to produce hibridoma in Bal B/c peritoneal? I want to produce mAb to bovin type II collagen.wish your help.