Antibodies to estrogen receptor, recommendations? - (Jul/12/2006 )
I am trying to do a western blot for estrogen receptors (alpha and beta) in cell lysates. I have bought antibodies from Santa Cruz and Upstate. I get a couple of bands (Santa Cruz) and alot of bands (Upstate) in my blot. Is that normal or is it because the antibodies are not specific? Any recommendations? Thanks
This is Santa Cruz Biotechnology. If you can let us know which antibodies you have, we may be able to help you decipher what those bands could be. The Estrogen Receptor does exist as a phosphorylated protein, which may shift the size on a Western blot. Without knowing exactly where the "couple of bands" are, it is hard to tell if that is what is happening in your case.
If phosphorylation is not the cause of the multiple bands, we may be able to help you optimize the performance of your antibodies. You can always contact us at firstname.lastname@example.org or at 1-800-457-3801 ext. 2.
Santa Cruz Biotechnology Technical Service and Customer Care