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MHC II molecules - antigen bound state - purchasable conformation specific antibodies (Aug/04/2008 )


is it somehow possible to determine amount of MHCII molecules which have an antigen bound?
e.g. some special anitbody which recognizes differential conformation or so?

thanks for suggestions


its not clear if you are asking if a specific peptide is presented or if there are 'empty' mhc II molecules on the cell sruface.

under normal conditions in a wild-type cell (DC, macrophage, or B cell), every single MHC II molecule (and class I for that matter) should have a peptide bound. i know that there are several Abs which bind class I bound to specific peptides, so i am pretty sure that similar reagents exist for class II.

-JE UMass IVP-