Antibodies for ChiP - Of mice and men (Jul/25/2007 )
Dear dpo,krümelmonster,and KPDE Thank you all for quick and nice replies
I have another question seems to be of same low level and its about histone acetylation and methylation. That is can we use the same antibodies for human and mouse in order to check acetylation and methylation by Chromatin immunoprecipitaion (ChIP), or we need different antibodies for both species or there are some which can be used for both and others which are species specific? How to find and select them? Which company you will suggest?
Thannks
w
-w007-
QUOTE (w007 @ Jul 25 2007, 10:10 PM)
Dear dpo,krümelmonster,and KPDE Thank you all for quick and nice replies
I have another question seems to be of same low level and its about histone acetylation and methylation. That is can we use the same antibodies for human and mouse in order to check acetylation and methylation by Chromatin immunoprecipitaion (ChIP), or we need different antibodies for both species or there are some which can be used for both and others which are species specific? How to find and select them? Which company you will suggest?
Thannks
w
I have another question seems to be of same low level and its about histone acetylation and methylation. That is can we use the same antibodies for human and mouse in order to check acetylation and methylation by Chromatin immunoprecipitaion (ChIP), or we need different antibodies for both species or there are some which can be used for both and others which are species specific? How to find and select them? Which company you will suggest?
Thannks
w
The companies should tell you what species an antibody works with or should work with (based on sequence identity). Additionally, with some companies there may be comments on the antibody from people who've used it so you can check and see if anyone has used it successfully for the species you're interested in.
We've used antibodies from Abcam (no problems), Cell Signaling (no problems), Upstate (these usually work), Santa Cruz (hit and miss), and Covance (hit and miss) for ChIP. Even though companies like Santa Cruz might be hit and miss for antibodies they may still sell the best antibody for a particular antigen. If you're looking for antibodies to a particular antigen you should look in the literature and find an antibody that many labs have used for that antigen.
-KPDE-