Removing LPS from mAb stocks - (Dec/01/2006 )
I need to remove LPS from my mAb stocks for my experimnts.
I did use the polymyxinB column from Peirce, but my samples were also bound!!
I think many people experienced this problem with polymyxin column.
Does anybody know any better way to do it?
I searched and heard about EndoTrap from profos. But I'm not sure if it's worth to try.
Has anyone used this product ever?
I don't wanna go through the ion exchange purification.....:/
I am Stephanie from Profos AG.
Kindly I am ready to help you with your special question; however I do not want to use this forum as advertisement for EndoTrap.
For any further information please contact Profos via our webpage („contact us“).
Methods how someone can remove endotoxins are:
Treatment of strong acid or alkali
Adsorption on activated carbon, asbestos or barium sulphate
Affinity chromatography (e.g. EndoTrap)