can u guess the molecular weight of a raised antibody? - (Mar/13/2007 )
well,i got a strech of peptide synthesized and antibody raised from it.is it a polyclonal antibody because in western blots it picks up many bands. can i ever know the molecular weight of this antibody. In general what is the molecular weight of an antibody.
The western shows you the MW of the protein that the antibody recognizes, not the MW of the antibody itself. I think the MW of antibodies are different depending on what type of Ig molecules they are made of, IgA, IgG, etc. I don't know if a table exists anywhere...
IgG heavy chain ~55 kDa, light chain ~25 kDa in SDS PAGE
For IgG between 150-190 kDA depend on glycosylation ( In sample buffer without bME on SDSPAAG ) with bME as The Bearer say