Source for PAR antibody? - IHC for Poly-ADP-Ribose on rat tissues (Jul/13/2005 )

Hello - Our research group needs to stain FFPE rat tissue samples for PAR (aka poly-ADP-ribose), not PARP (poly-ADP-ribose polymerase). We had an antibody that worked very well, unfortunately the rabbit died and new lots have shown very poor staining (the manufacturer agrees that they have a problem).

The PI for this project (we are a chemistry group) mentioned our problems at a recent meeting, another researcher mentioned that she had a source in Japan for PAR antibody which was expensive, but gave very repeatable and reliable staining. She was supposed to e-mail him with details, but he hasn't heard anything.

Does anyone have a PAR staining protocol that works on FFPE rat tissue?

Thanks!

-eluned-

have a look at

www.abcam.com

they have a huge databes of differnt antibodies....

if you enter "poly-ADP-ribose" in the search, some results show up , the first one is:

ab14460

PADPR antibody
Poly ADP-ribose

Chicken

Polyclonal

ELISA, IHC, IHC-Fr, WB

Expected to cross-react with a wide range of species due to sequence homology.

mike

-jadefalcon-

QUOTE (jadefalcon @ Jul 14 2005, 12:41 PM)

have a look at

www.abcam.com
they have a huge databes of differnt antibodies....

if you enter "poly-ADP-ribose" in the search, some results show up , the first one is:

ab14460
PADPR antibody
Poly ADP-ribose

Chicken
Polyclonal
ELISA, IHC, IHC-Fr, WB
Expected to cross-react with a wide range of species due to sequence homology.

mike

Thanks - Abcam also has a mouse monoclonal for PAR available.
Biocompare.com has another very nice antibody database which lists Tulip BioLabs(polyclonal and monoclonal), Serotec, GenTex, ADI and BDPharmingen (whose new lots no longer work for us). The PhD who has been working on this progect is out of the country at the moment, but I know she tried a couple US suppliers other than BD.

I'm hoping to get in touch either with someone who can pinpoint this Japanese source OR someone who is using one of the more readily available antibodies of FFPE rat tissue. We are a chemistry group, so rely on a histology core to process our samples. From what I understand, IHC staining protocols often need tweaking to optimize results. If there is a protocol already worked out for the readily available antibodies, it would be worth another try. (FWIW, the tissues we are looking at include rat heart, pancreas, liver and thymus.)

-eluned-