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what kind of secondary antibody do i need? - (Sep/10/2008 )

Hello,

I am trying to detect the presence of an unknown auto-antibody in patient sera. I will be doing a few Western blots to see if I can pick up a novel band suggesting an immune process. The primary antibody of course will the patient serum. What kind of secondary antibody would be best to order? I was thinking a anti-human IgG but I'm not sure. Any suggestions?

Thanks

-medstudent1-

QUOTE (medstudent1 @ Sep 10 2008, 08:33 AM)
Hello,

I am trying to detect the presence of an unknown auto-antibody in patient sera. I will be doing a few Western blots to see if I can pick up a novel band suggesting an immune process. The primary antibody of course will the patient serum. What kind of secondary antibody would be best to order? I was thinking a anti-human IgG but I'm not sure. Any suggestions?

Thanks


for an early response also IgM, allergic also IgE

-The Bearer-