"recombinanant antibody" - (Jan/23/2008 )
Hi all,
I am wondering, is it possible to get the DNA sequence of antibody and transfect it with a vector into B cells and the B cells would produce the desired antibody. Does anyone do or read about this kinda experiment? (or is that impossible?)Thank you.
Regards,
Wllmch
well, what is the purpose of your experiment? to make some more antibody? I would think making a hybridoma would be easier. that's a very complicated thing to clone
Yes. But i am thinking of making some "humanized" antibodies. Thats why thinking of doing a transfection. Is that the way to do it?
From what I remember of my Immunology class, mice are commonly used to establish cell lines that generate antibodies. If one wants humanized antibodies, one takes the cell line (which has presumably been immortalized), find the genes for the antibody, swap out the murine Fc region (or, if you want to do more work, all but the variable regions of the antigen binding site), stick the two genes into your mammalian cell line of choice with a decent promoter, and collect your buckets of antibody.
There now exist mice whose own antibody genes have been knocked out and replaced with human antibody genes, so they produce human antibodies with no need to be humanized later.