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Can we reuse the primary antibody in immunohistochemistry? - (Jun/27/2008 )

I know that we can reuse primary antibody in western blot, but I'm wondering if it's the case in immunohistochemistry as well or not? because this technique needs a lot of primary depending on how many slides we have to work with ... If we can, for how long?


I think you can reuse for immunohistochemistry until you can see the weak signals (meaning it is time to add more primary antibody!)


I've reused primary antibody from immunohistochemistry. As timjim said, use until you see weak signals.


great ... !!! Thank you ...