Rainbow MW marker from Amersham - detection with a mouse secondary antibody? (Jun/21/2007 )
Would you know by any chance if the Rainbow molecular weight marker from amersham is supposed to react with a mouse secondary antibody?
That might sound as a strange question, but one of my collegue is positive that some of th bands are supposed to be detected, and I can't see anything on my western (this marker was supposed to be my positive control). In the product description from the amersham website, I could not find any information regarding that matter. The recombinant protein are his-tagged, but nothing concerning a mouse secondary antibody...
We use the Rainbow marker from amersham, and I have never seen anybands being recognize by the secondary mouse antibody. The only time one of the marker bands react is when they are recognized by the primary antibody.