Hi everyone,

I've been searching secondary antibodies for use in immunofluorescence and I came across two new types of fluorochromes:

does anyone have experience with the new DyLights from Rockland or NorthernLights from RnD systems?

Another thing: which are, in your opinion, the best fluorescence conjugated antibodies in the market?

Waiting for your opinions!

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we use Alexa conjugated antibodies. They are good.

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alexa secondaries from molecular probes are good.

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We use the alexa secondaries that are highly cross-absorbed from molecular probes. A few more dollars for the extra purification but I have great success with these. Nice clean staining with minimal background.

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Thanks everyone for your opinions.

I'll keep them in mind!

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We use Jackson ImmunoResearch secondaries raised in donkey - work great and no cross-reactivity.

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I use Alexa and I've had mixed success. It's been hit and miss, I found some AMAZING ones with no background and no non-specific binding, but there were a couple that had terrible non-specific binding so no matter what run your controls.

I have also used jackson raised in donkey and had no problems, they just aren't as bright as alexa.

I am curious about trying dylights and since i do tons of IF I'll probably try them next

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