vortexing of antibodies upon dilution in 3% skim milk - (Jan/24/2007 )
I am a beginner in western blot. is it possible to vortex antibodies (primery &secondary) upon dilution in 3% skim milk ? or is it better to mix by pipetting up and down?
I just mix them by inverting. Vortexing is not neccessary and may harm them.
I vortex antibody sol. in milk, gently and briefly for 1-2 seconds.
I do the same as scolix