Monoclonal Ab is always from mice? - (Jul/13/2006 )

Hello to all!

Is it true that there only exists monoclonal antibodies from mouse origin?
If we have a goat or rabbit antibody, we can be sure it is a policlonal antisera?

Thanx

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There is also some rat monoclonal antibodies.
when you want to make monoclonal antibodies, you use mouse (or rat), because fusion protocols are OK. If you want some polyclonal, you have to take blood, then you use bigger animals, like rabbit, goat and donkey.
I've never heard about rabbit monoclonal antibodies.
I would say there is 99.9% chance that a mouse is monoclonal, and rabbit polyclonal.

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No,
in the last years scientist developed new techniques to produce monoclonal antibodys as well from rabbit (look here), goat, rat, horse...
So you can´t be sure with your Abs!

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hi guys,

R&D is one of the companies which is producing rabbit monoclonal antibodies.

when u use serum, those are polyclonal antibodies ofcourse.

so try to use anti rabbit antibody as secondary, u will get ur results for sure.

gud luck

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