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Antibody Substitutes - (Jun/16/2005 )

Hi folks,
I am compiling a comprehensive list of antibody substitutes espcially with regard to
1) their effectivity as a tool to study protiens/pathways etc?
2) their cost of product compared to simple antibodies and more complex valued added ones
3) Current usage of Ab substitutes in academic/biotech/pharma circles
4) Other advantages or disadvatages they may over antibodies
Most of my searches came up with substitutes called aptamers however there doesnt seem to be much literature on them post 2000-2001

Any help would be appreciated



Hi there!

If you go to the PubMed and use thier search with "aptamers" and 2002-2005, you'll get >300 papers with >50 reviews....

example (just the first 21):

1: Borghouts C, Kunz C, Groner B.
Peptide aptamers: recent developments for cancer therapy.
Expert Opin Biol Ther. 2005 Jun;5(6):783-97.
PMID: 15952909 [PubMed - in process]

2: Marro ML, Daniels DA, McNamee A, Andrew DP, Chapman TD, Jiang MS, Wu Z,
Smith JL, Patel KK, Gearing KL.
Identification of Potent and Selective RNA Antagonists of the
IFN-gamma-Inducible CXCL10 Chemokine.
Biochemistry. 2005 Jun 14;44(23):8449-8460.
PMID: 15938634 [PubMed - as supplied by publisher]

3: Moshfeghi AA, Puliafito CA.
Pegaptanib sodium for the treatment of neovascular age-related macular
Expert Opin Investig Drugs. 2005 May;14(5):671-82.
PMID: 15926872 [PubMed - in process]

4: Cong X, Nilsen-Hamilton M.
Allosteric aptamers: targeted reversibly attenuated probes.
Biochemistry. 2005 Jun 7;44(22):7945-54.
PMID: 15924413 [PubMed - in process]

5: Wang J, Jiang Y, Zhou C, Fang X.
Aptamer-based ATP assay using a luminescent light switching complex.
Anal Chem. 2005 Jun 1;77(11):3542-6.
PMID: 15924387 [PubMed - in process]

6: Stadtherr K, Wolf H, Lindner P.
An aptamer-based protein biochip.
Anal Chem. 2005 Jun 1;77(11):3437-43.
PMID: 15924373 [PubMed - in process]

7: Dey AK, Griffiths C, Lea SM, James W.
Structural characterization of an anti-gp120 RNA aptamer that neutralizes R5
strains of HIV-1.
RNA. 2005 Jun;11(6):873-84.
PMID: 15923374 [PubMed - in process]

8: Gevertz J, Gan HH, Schlick T.
In vitro RNA random pools are not structurally diverse: a computational
RNA. 2005 Jun;11(6):853-63.
PMID: 15923372 [PubMed - in process]

9: Tucker BJ, Breaker RR.
Riboswitches as versatile gene control elements.
Curr Opin Struct Biol. 2005 May 23; [Epub ahead of print]
PMID: 15919195 [PubMed - as supplied by publisher]

10: Kato Y, Minakawa N, Komatsu Y, Kamiya H, Ogawa N, Harashima H, Matsuda A.
New NTP analogs: the synthesis of 4'-thioUTP and 4'-thioCTP and their utility
for SELEX.
Nucleic Acids Res. 2005 May 24;33(9):2942-51. Print 2005.
PMID: 15914669 [PubMed - indexed for MEDLINE]

11: Misono TS, Kumar PK.
Selection of RNA aptamers against human influenza virus hemagglutinin using
surface plasmon resonance.
Anal Biochem. 2005 May 21; [Epub ahead of print]
PMID: 15913532 [PubMed - as supplied by publisher]

12: Surugiu-Warnmark I, Warnmark A, Toresson G, Gustafsson JK, Bulow L.
Selection of DNA aptamers against rat liver X receptors.
Biochem Biophys Res Commun. 2005 Jul 1;332(2):512-7.
PMID: 15910755 [PubMed - in process]

13: Ecale Zhou CL, Zemla AT, Roe D, Young M, Lam M, Schoeniger JS, Balhorn R.
Computational approaches for identification of conserved/unique binding pockets
in the A chain of ricin.
Bioinformatics. 2005 May 19; [Epub ahead of print]
PMID: 15905278 [PubMed - as supplied by publisher]

14: Chou SH, Chin KH, Wang AH.
DNA aptamers as potential anti-HIV agents.
Trends Biochem Sci. 2005 May;30(5):231-4.
PMID: 15896739 [PubMed - in process]

15: Cao Z, Tan W.
Molecular Aptamers for Real-Time Protein-Protein Interaction Study.
Chemistry. 2005 May 13; [Epub ahead of print]
PMID: 15892184 [PubMed - as supplied by publisher]

16: Heyduk E, Heyduk T.
Nucleic acid-based fluorescence sensors for detecting proteins.
Anal Chem. 2005 Feb 15;77(4):1147-56.
PMID: 15858998 [PubMed - in process]

17: Tombelli S, Minunni M, Mascini M.
Analytical applications of aptamers.
Biosens Bioelectron. 2005 Jun 15;20(12):2424-34. Epub 2004 Dec 16.
PMID: 15854817 [PubMed - in process]

18: Joshi PJ, North TW, Prasad VR.
Aptamers directed to HIV-1 reverse transcriptase display greater efficacy over
small hairpin RNAs targeted to viral RNA in blocking HIV-1 replication.
Mol Ther. 2005 May;11(5):677-86.
PMID: 15851006 [PubMed - in process]

19: [No authors listed]
[Methods of experimental validation of potential target proteins for creation
of new drugs]
Biomed Khim. 2005;51(1):2-18. Russian.
PMID: 15850214 [PubMed - in process]

20: Famulok M.
Allosteric aptamers and aptazymes as probes for screening approaches.
Curr Opin Mol Ther. 2005 Apr;7(2):137-43.
PMID: 15844621 [PubMed - in process]

21: Cerchia L, Duconge F, Pestourie C, Boulay J, Aissouni Y, Gombert K, Tavitian
B, de Franciscis V, Libri D.
Neutralizing aptamers from whole-cell SELEX inhibit the RET receptor tyrosine
PLoS Biol. 2005 Apr;3(4):e123. Epub 2005 Mar 22.
PMID: 15769183 [PubMed - in process]