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Couple peptide to beads? for antibody purification - (Sep/17/2007 )

Hello experts,

I am trying to couple a peptide (approx. 35 amino acids long) to beads. This will be later used for affinity purification of antibodies.

Can anyone suggest a good protocol for this coupling? Or direct me to a reliable source?



what kind of beads are you going to use? cnbr-sepharose? ech-sepharose? eah-sepharose?

are you going to activate the bead yourself?

there is a free manual available for download (i can't upload it for you from this computer) at the gehealthcare website. this will give you all the information you need to do the job.