How can I elute antigen from proteinG+antibody+antigen complexes? - (Nov/29/2006 )

I want to just elute proteins from protein-antibody-proteinG beads without any antibody. What kind of buffer should I use to elute protein and keep antibody still attaching on protein G beads?
Thanks!

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Normally, when you elute the protein from the protein G beads, you will also elute the antibody.
Therefore you will need to couple the antibody to the beads using kits, so that only the protein will be eluted.

Hope this help.

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I agree with Minnie Mouse. However coupling can cause a whole new slew of problems such as masking the antigenic site that your protein binds to. Also, coupling usually isn't 100% so you may still get some of the antibody eluting. It's still worth a try but just be aware of the problems associated with it.

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what is it for? maybe there are some other ways to avoid problems with the eluted antibodies.
are you purifying antigens, or do you want to do immunoprecipitation for western-blot?

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