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Question on the recommended application of primary Antibody - (May/07/2006 )

I have some primary antibodies recommended for IF IP or WB,even the IHC(Frozen section), But not for IHC(P), I want to know why the antibody can't be used for the IHC(P) ?Is there any professional explation? Why the antigen being retrieved,there is still no positive staining on the specimens?



hope this will helps u.

gud luk


QUOTE (payeli @ May 7 2006, 07:24 AM)

Thanks for your suggestions.
Can u explain to me why most antibodies are not recommended for IHC(P)? Just not tested?