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Top : New Forum Archives (2009-): : Molecular-Biology
361. Principle of silica membrane binding of nucleic acid - (reply: 6)
362. genogroup and genotype - (reply: 2)
363. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
364. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
365. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
366. Beta alanine alternative? - (reply: 2)
367. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
368. lncRNA full-sequence or partial sequence - (reply: 2)
369. Cloning at 6 kb gene - (reply: 3)
370. Trouble with DNA purity (A260/A280) - (reply: 3)
371. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
372. Luciferase assay - extreme variation in RLU - (reply: 1)
373. site-directed mutagenesis - (reply: 20)
374. qPCR detection - (reply: 2)
375. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
376. TCA precipitation - (reply: 1)
377. SOC vs TB in transformations - (reply: 1)
378. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
379. Single RE site cloning - (reply: 3)
380. PCR product running on agarose gel - (reply: 32)
381. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
382. PCR amplification with high fidelity enzyme - (reply: 1)
383. Transformation problem - (reply: 5)
384. finding gene sequence to delete - (reply: 4)
385. Blue fluorescent color of agarose gel in UV - (reply: 7)
386. heterologous gene integration into e.coli genome - (reply: 1)
387. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
388. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
389. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
390. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)