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Top : New Forum Archives (2009-): : Molecular-Biology
361. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
362. Luciferase assay - extreme variation in RLU - (reply: 1)
363. site-directed mutagenesis - (reply: 20)
364. qPCR detection - (reply: 2)
365. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
366. TCA precipitation - (reply: 1)
367. SOC vs TB in transformations - (reply: 1)
368. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
369. Single RE site cloning - (reply: 3)
370. PCR product running on agarose gel - (reply: 32)
371. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
372. PCR amplification with high fidelity enzyme - (reply: 1)
373. Transformation problem - (reply: 5)
374. finding gene sequence to delete - (reply: 4)
375. Blue fluorescent color of agarose gel in UV - (reply: 7)
376. heterologous gene integration into e.coli genome - (reply: 1)
377. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
378. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
379. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
380. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
381. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
382. Continuous growth of E. coli on AMP LB plate - (reply: 2)
383. Plasmid compatibly groups - (reply: 5)
384. too much DNA on agarose gel - (reply: 9)
385. What are the "mammalian vectors N1 and C1"? - (reply: 1)
386. Life before BLAST etc? Help needed... - (reply: 1)
387. How base pairs upstream of gene to include for the promoter? - (reply: 3)
388. Neomycin resistance sequence - (reply: 1)
389. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
390. polyacrylamide RNA gel - (reply: 4)