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2161. Pfu is Pol I or Pol II ?? - Silly Q.s. (reply: 1)
2162. how to determine certain gene copy number? - (reply: 3)
2163. in situs: Fixed and store for too long? - (reply: 1)
2164. Phenol - why different for DNA and RNA extraction? (reply: 9)
2165. PCR, RNA, Northern Blotting???? - (reply: 1)
2166. concentration of dna - (reply: 3)
2167. Northern blot: Wetting a membrane - oops... (reply: 1)
2168. bizzare PCR smear - why does science hate me (reply: 7)
2169. DNA dissolved in DMSO instead of H2O - (reply: 2)
2170. Running Polyacrylamide gel in acidic conditions - Adicid denaturing PAGE (with or without SD?) (reply: 1)
2171. identifying isoforms - (reply: 1)
2172. BamHI restriction digest - (reply: 9)
2173. Sub-cloning sticky-end PCR products - (reply: 2)
2174. Fragmented DNA doesn't appear as bands on agarose gel - (reply: 5)
2175. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
2176. Judging a dissociation curve - (reply: 2)
2177. Knock-out MEFs and WT MEFs - (reply: 2)
2178. transfecting TOPO vector - can it be done? (reply: 6)
2179. 2 step RNA isolation - Possible to Isolate RNA in 2 days? (reply: 2)
2180. lentiviral transdcution of hematopoëtic stem cells - (reply: 2)
2181. Questions about 260/230 when reading DNA conc. - (reply: 1)
2182. Transcribed complementary sequence - (reply: 3)
2183. Quikchange Mutagenesis Help Please!!! - (reply: 7)
2184. North-Western Blot - (reply: 1)
2185. Genomic DNA contamination of RNA - (reply: 24)
2186. blunt end ligation problem - (reply: 2)
2187. Non-specific bands on EMSA - (reply: 2)
2188. restriction enzyme problem - restriction enzyme problem (reply: 4)
2189. Always a Band in negative after RT-PCR - RT-PCR and Gapdh (reply: 2)
2190. Gel extraction help.... desperate! - (reply: 6)
2162. how to determine certain gene copy number? - (reply: 3)
2163. in situs: Fixed and store for too long? - (reply: 1)
2164. Phenol - why different for DNA and RNA extraction? (reply: 9)
2165. PCR, RNA, Northern Blotting???? - (reply: 1)
2166. concentration of dna - (reply: 3)
2167. Northern blot: Wetting a membrane - oops... (reply: 1)
2168. bizzare PCR smear - why does science hate me (reply: 7)
2169. DNA dissolved in DMSO instead of H2O - (reply: 2)
2170. Running Polyacrylamide gel in acidic conditions - Adicid denaturing PAGE (with or without SD?) (reply: 1)
2171. identifying isoforms - (reply: 1)
2172. BamHI restriction digest - (reply: 9)
2173. Sub-cloning sticky-end PCR products - (reply: 2)
2174. Fragmented DNA doesn't appear as bands on agarose gel - (reply: 5)
2175. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
2176. Judging a dissociation curve - (reply: 2)
2177. Knock-out MEFs and WT MEFs - (reply: 2)
2178. transfecting TOPO vector - can it be done? (reply: 6)
2179. 2 step RNA isolation - Possible to Isolate RNA in 2 days? (reply: 2)
2180. lentiviral transdcution of hematopoëtic stem cells - (reply: 2)
2181. Questions about 260/230 when reading DNA conc. - (reply: 1)
2182. Transcribed complementary sequence - (reply: 3)
2183. Quikchange Mutagenesis Help Please!!! - (reply: 7)
2184. North-Western Blot - (reply: 1)
2185. Genomic DNA contamination of RNA - (reply: 24)
2186. blunt end ligation problem - (reply: 2)
2187. Non-specific bands on EMSA - (reply: 2)
2188. restriction enzyme problem - restriction enzyme problem (reply: 4)
2189. Always a Band in negative after RT-PCR - RT-PCR and Gapdh (reply: 2)
2190. Gel extraction help.... desperate! - (reply: 6)