|
241. rescue cloning of a transposon mutant - (reply: 2)
242. Dephosphorylation - (reply: 7)
243. study of receptors in cell lines - (reply: 1)
244. Mouse genotyping issues - (reply: 1)
245. In-situ hybridization - (reply: 1)
246. His tag introduction in to gene and primer design - (reply: 2)
247. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
248. Principle of silica membrane binding of nucleic acid - (reply: 6)
249. genogroup and genotype - (reply: 2)
250. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
251. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
252. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
253. Beta alanine alternative? - (reply: 2)
254. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
255. lncRNA full-sequence or partial sequence - (reply: 2)
256. Cloning at 6 kb gene - (reply: 3)
257. Trouble with DNA purity (A260/A280) - (reply: 3)
258. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
259. Luciferase assay - extreme variation in RLU - (reply: 1)
260. site-directed mutagenesis - (reply: 20)
261. qPCR detection - (reply: 2)
262. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
263. TCA precipitation - (reply: 1)
264. SOC vs TB in transformations - (reply: 1)
265. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
266. Single RE site cloning - (reply: 3)
267. PCR product running on agarose gel - (reply: 32)
268. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
269. PCR amplification with high fidelity enzyme - (reply: 1)
270. Transformation problem - (reply: 5)
242. Dephosphorylation - (reply: 7)
243. study of receptors in cell lines - (reply: 1)
244. Mouse genotyping issues - (reply: 1)
245. In-situ hybridization - (reply: 1)
246. His tag introduction in to gene and primer design - (reply: 2)
247. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
248. Principle of silica membrane binding of nucleic acid - (reply: 6)
249. genogroup and genotype - (reply: 2)
250. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
251. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
252. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
253. Beta alanine alternative? - (reply: 2)
254. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
255. lncRNA full-sequence or partial sequence - (reply: 2)
256. Cloning at 6 kb gene - (reply: 3)
257. Trouble with DNA purity (A260/A280) - (reply: 3)
258. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
259. Luciferase assay - extreme variation in RLU - (reply: 1)
260. site-directed mutagenesis - (reply: 20)
261. qPCR detection - (reply: 2)
262. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
263. TCA precipitation - (reply: 1)
264. SOC vs TB in transformations - (reply: 1)
265. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
266. Single RE site cloning - (reply: 3)
267. PCR product running on agarose gel - (reply: 32)
268. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
269. PCR amplification with high fidelity enzyme - (reply: 1)
270. Transformation problem - (reply: 5)