Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
1081. Mutagenesis, deletion - what is the best kit/protocol (reply: 6)
1082. Ethidium Bromide Stability - (reply: 1)
1083. alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
1084. primer design - microalgae (reply: 2)
1085. Trouble to UNDERSTAND gene replacement results - (reply: 1)
1086. Would clean dry hot air kill bacteria? - (reply: 6)
1087. Genomic DNA contamination in my RNA sample? - (reply: 8)
1088. Cloning and expressing a mystery 71KDa protein - (reply: 9)
1089. why 12 h culture for plasmid prep? - (reply: 2)
1090. Insert magically shortening??? - Cloning issues (reply: 2)
1091. Transformation issue - (reply: 1)
1092. RNase should be added in which step for gDNA isolation using phenol chloroform m - after lysozyme / preteinase k treatment or after phenol chloroform? (reply: 9)
1093. PCR yield in ng/ul - (reply: 1)
1094. How to add a T7 terminator to a vector? - (reply: 4)
1095. Dna after gel extraction, single or double stranded? - (reply: 1)
1096. Checking RNA on gel after DIG labeling - (reply: 1)
1097. Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
1098. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
1099. DNA-PK inhibitor - Which chemical is the best? (reply: 1)
1100. some mathematics help - meaning of 5.0E+03 (reply: 4)
1101. Storage conditions - (reply: 4)
1102. is it degraded RNA? - I dont get a clear 3-band after electrophoresis (reply: 6)
1103. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
1104. Stability of DNA at RT - (reply: 1)
1105. yeast expression (gal1-promotor) - expression in glucose?? (reply: 1)
1106. TRIZol Reagent additional reagents & protocol clarifications - (reply: 4)
1107. Is it possible to islate strong negative charged protein - (reply: 1)
1108. DIG southern blot - (reply: 2)
1109. Real time PCR in the presence of heme - (reply: 3)
1110. Protein expression vectors Please help - (reply: 2)