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Top : New Forum Archives (2009-): : Molecular-Biology
511. leaky promoter - (reply: 1)
512. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
513. SAP dephosphorylation - (reply: 1)
514. Determining in frame in modified vector - (reply: 1)
515. Deleting restriction enzyme sites - (reply: 1)
516. Creating primers to add restriction sites to vector backbone - (reply: 7)
517. cDNA test on 1% agarose gel - (reply: 3)
518. Plasmid isolation from gram positive bacteria - (reply: 2)
519. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
520. rsaI enzyme - (reply: 1)
521. How to check for the presence of plasmid? - (reply: 3)
522. Extracting total RNA from woody plant roots - (reply: 2)
523. Quantify total DNA in small cell lysate...? - (reply: 1)
524. Quantify DNA in gel electrophoresis bands - (reply: 7)
525. Protein extraction and isolectric focusing - (reply: 1)
526. getting strange data in Luciferase-renilla assay - (reply: 2)
527. Homozygote DNA but Heterozygote RNA? - (reply: 2)
528. sacB Counter-Selection to generate double cross-over deletion mutants - (reply: 8)
529. TA Cloning of alpha-1,3 glucanase: No blue colonies - (reply: 5)
530. What is the necessary time for lipofectamin to make complex with pre-miRNA oligo - (reply: 1)
531. what is the ORFeome clone? - (reply: 2)
532. ligation with one ristrection site/enzyme - (reply: 3)
533. No amplification during RT-PCR - (reply: 4)
534. rescue cloning of a transposon mutant - (reply: 2)
535. Dephosphorylation - (reply: 7)
536. study of receptors in cell lines - (reply: 1)
537. Mouse genotyping issues - (reply: 1)
538. In-situ hybridization - (reply: 1)
539. His tag introduction in to gene and primer design - (reply: 2)
540. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)