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Top : New Forum Archives (2009-): : Molecular-Biology
511. pcr 10X buffer preparation - (reply: 2)
512. Sensitive, Consistent and Reproducible Method(s) for Extraction of gDNA from Low - (reply: 2)
513. Enhancer Analysis - (reply: 2)
514. how much template do i need for pcr? - (reply: 5)
515. 5'-azacytidine treatment - (reply: 1)
516. leaky promoter - (reply: 1)
517. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
518. SAP dephosphorylation - (reply: 1)
519. Determining in frame in modified vector - (reply: 1)
520. Deleting restriction enzyme sites - (reply: 1)
521. Creating primers to add restriction sites to vector backbone - (reply: 7)
522. cDNA test on 1% agarose gel - (reply: 3)
523. Plasmid isolation from gram positive bacteria - (reply: 2)
524. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
525. rsaI enzyme - (reply: 1)
526. How to check for the presence of plasmid? - (reply: 3)
527. Extracting total RNA from woody plant roots - (reply: 2)
528. Quantify total DNA in small cell lysate...? - (reply: 1)
529. Quantify DNA in gel electrophoresis bands - (reply: 7)
530. Protein extraction and isolectric focusing - (reply: 1)
531. getting strange data in Luciferase-renilla assay - (reply: 2)
532. Homozygote DNA but Heterozygote RNA? - (reply: 2)
533. sacB Counter-Selection to generate double cross-over deletion mutants - (reply: 8)
534. TA Cloning of alpha-1,3 glucanase: No blue colonies - (reply: 5)
535. What is the necessary time for lipofectamin to make complex with pre-miRNA oligo - (reply: 1)
536. what is the ORFeome clone? - (reply: 2)
537. ligation with one ristrection site/enzyme - (reply: 3)
538. No amplification during RT-PCR - (reply: 4)
539. rescue cloning of a transposon mutant - (reply: 2)
540. Dephosphorylation - (reply: 7)