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Top : New Forum Archives (2009-): : Molecular-Biology
511. genogroup and genotype - (reply: 2)
512. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
513. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
514. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
515. Beta alanine alternative? - (reply: 2)
516. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
517. lncRNA full-sequence or partial sequence - (reply: 2)
518. Cloning at 6 kb gene - (reply: 3)
519. Trouble with DNA purity (A260/A280) - (reply: 3)
520. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
521. Luciferase assay - extreme variation in RLU - (reply: 1)
522. site-directed mutagenesis - (reply: 20)
523. qPCR detection - (reply: 2)
524. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
525. TCA precipitation - (reply: 1)
526. SOC vs TB in transformations - (reply: 1)
527. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
528. Single RE site cloning - (reply: 3)
529. PCR product running on agarose gel - (reply: 32)
530. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
531. PCR amplification with high fidelity enzyme - (reply: 1)
532. Transformation problem - (reply: 5)
533. finding gene sequence to delete - (reply: 4)
534. Blue fluorescent color of agarose gel in UV - (reply: 7)
535. heterologous gene integration into e.coli genome - (reply: 1)
536. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
537. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
538. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
539. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
540. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)