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Top : New Forum Archives (2009-): : Molecular-Biology
511. Cloning at 6 kb gene - (reply: 3)
512. Trouble with DNA purity (A260/A280) - (reply: 3)
513. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
514. Luciferase assay - extreme variation in RLU - (reply: 1)
515. site-directed mutagenesis - (reply: 20)
516. qPCR detection - (reply: 2)
517. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
518. TCA precipitation - (reply: 1)
519. SOC vs TB in transformations - (reply: 1)
520. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
521. Single RE site cloning - (reply: 3)
522. PCR product running on agarose gel - (reply: 32)
523. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
524. PCR amplification with high fidelity enzyme - (reply: 1)
525. Transformation problem - (reply: 5)
526. finding gene sequence to delete - (reply: 4)
527. Blue fluorescent color of agarose gel in UV - (reply: 7)
528. heterologous gene integration into e.coli genome - (reply: 1)
529. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
530. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
531. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
532. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
533. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
534. Continuous growth of E. coli on AMP LB plate - (reply: 2)
535. Plasmid compatibly groups - (reply: 5)
536. too much DNA on agarose gel - (reply: 10)
537. What are the "mammalian vectors N1 and C1"? - (reply: 1)
538. Life before BLAST etc? Help needed... - (reply: 1)
539. How base pairs upstream of gene to include for the promoter? - (reply: 3)
540. Neomycin resistance sequence - (reply: 1)