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Top : New Forum Archives (2009-): : Molecular-Biology
511. TA Cloning of alpha-1,3 glucanase: No blue colonies - (reply: 5)
512. What is the necessary time for lipofectamin to make complex with pre-miRNA oligo - (reply: 1)
513. what is the ORFeome clone? - (reply: 2)
514. ligation with one ristrection site/enzyme - (reply: 3)
515. No amplification during RT-PCR - (reply: 4)
516. rescue cloning of a transposon mutant - (reply: 2)
517. Dephosphorylation - (reply: 7)
518. study of receptors in cell lines - (reply: 1)
519. Mouse genotyping issues - (reply: 1)
520. In-situ hybridization - (reply: 1)
521. His tag introduction in to gene and primer design - (reply: 2)
522. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
523. Principle of silica membrane binding of nucleic acid - (reply: 6)
524. genogroup and genotype - (reply: 2)
525. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
526. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
527. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
528. Beta alanine alternative? - (reply: 2)
529. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
530. lncRNA full-sequence or partial sequence - (reply: 2)
531. Cloning at 6 kb gene - (reply: 3)
532. Trouble with DNA purity (A260/A280) - (reply: 3)
533. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
534. Luciferase assay - extreme variation in RLU - (reply: 1)
535. site-directed mutagenesis - (reply: 20)
536. qPCR detection - (reply: 2)
537. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
538. TCA precipitation - (reply: 1)
539. SOC vs TB in transformations - (reply: 1)
540. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)