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Top : New Forum Archives (2009-): : Molecular-Biology
511. what is the ORFeome clone? - (reply: 2)
512. ligation with one ristrection site/enzyme - (reply: 3)
513. No amplification during RT-PCR - (reply: 4)
514. rescue cloning of a transposon mutant - (reply: 2)
515. Dephosphorylation - (reply: 7)
516. study of receptors in cell lines - (reply: 1)
517. Mouse genotyping issues - (reply: 1)
518. In-situ hybridization - (reply: 1)
519. His tag introduction in to gene and primer design - (reply: 2)
520. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
521. Principle of silica membrane binding of nucleic acid - (reply: 6)
522. genogroup and genotype - (reply: 2)
523. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
524. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
525. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
526. Beta alanine alternative? - (reply: 2)
527. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
528. lncRNA full-sequence or partial sequence - (reply: 2)
529. Cloning at 6 kb gene - (reply: 3)
530. Trouble with DNA purity (A260/A280) - (reply: 3)
531. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
532. Luciferase assay - extreme variation in RLU - (reply: 1)
533. site-directed mutagenesis - (reply: 20)
534. qPCR detection - (reply: 2)
535. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
536. TCA precipitation - (reply: 1)
537. SOC vs TB in transformations - (reply: 1)
538. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
539. Single RE site cloning - (reply: 3)
540. PCR product running on agarose gel - (reply: 32)