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Top : New Forum Archives (2009-): : Molecular-Biology
511. Deleting restriction enzyme sites - (reply: 1)
512. Creating primers to add restriction sites to vector backbone - (reply: 7)
513. cDNA test on 1% agarose gel - (reply: 3)
514. Plasmid isolation from gram positive bacteria - (reply: 2)
515. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
516. rsaI enzyme - (reply: 1)
517. How to check for the presence of plasmid? - (reply: 3)
518. Extracting total RNA from woody plant roots - (reply: 2)
519. Quantify total DNA in small cell lysate...? - (reply: 1)
520. Quantify DNA in gel electrophoresis bands - (reply: 7)
521. Protein extraction and isolectric focusing - (reply: 1)
522. getting strange data in Luciferase-renilla assay - (reply: 2)
523. Homozygote DNA but Heterozygote RNA? - (reply: 2)
524. sacB Counter-Selection to generate double cross-over deletion mutants - (reply: 8)
525. TA Cloning of alpha-1,3 glucanase: No blue colonies - (reply: 5)
526. What is the necessary time for lipofectamin to make complex with pre-miRNA oligo - (reply: 1)
527. what is the ORFeome clone? - (reply: 2)
528. ligation with one ristrection site/enzyme - (reply: 3)
529. No amplification during RT-PCR - (reply: 4)
530. rescue cloning of a transposon mutant - (reply: 2)
531. Dephosphorylation - (reply: 7)
532. study of receptors in cell lines - (reply: 1)
533. Mouse genotyping issues - (reply: 1)
534. In-situ hybridization - (reply: 1)
535. His tag introduction in to gene and primer design - (reply: 2)
536. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
537. Principle of silica membrane binding of nucleic acid - (reply: 6)
538. genogroup and genotype - (reply: 2)
539. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
540. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)