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Top : New Forum Archives (2009-): : Molecular-Biology
511. rescue cloning of a transposon mutant - (reply: 2)
512. Dephosphorylation - (reply: 7)
513. study of receptors in cell lines - (reply: 1)
514. Mouse genotyping issues - (reply: 1)
515. In-situ hybridization - (reply: 1)
516. His tag introduction in to gene and primer design - (reply: 2)
517. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
518. Principle of silica membrane binding of nucleic acid - (reply: 6)
519. genogroup and genotype - (reply: 2)
520. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
521. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
522. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
523. Beta alanine alternative? - (reply: 2)
524. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
525. lncRNA full-sequence or partial sequence - (reply: 2)
526. Cloning at 6 kb gene - (reply: 3)
527. Trouble with DNA purity (A260/A280) - (reply: 3)
528. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
529. Luciferase assay - extreme variation in RLU - (reply: 1)
530. site-directed mutagenesis - (reply: 20)
531. qPCR detection - (reply: 2)
532. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
533. TCA precipitation - (reply: 1)
534. SOC vs TB in transformations - (reply: 1)
535. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
536. Single RE site cloning - (reply: 3)
537. PCR product running on agarose gel - (reply: 32)
538. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
539. PCR amplification with high fidelity enzyme - (reply: 1)
540. Transformation problem - (reply: 5)