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Top : New Forum Archives (2009-): : Molecular-Biology
511. Homozygote DNA but Heterozygote RNA? - (reply: 2)
512. sacB Counter-Selection to generate double cross-over deletion mutants - (reply: 8)
513. TA Cloning of alpha-1,3 glucanase: No blue colonies - (reply: 5)
514. What is the necessary time for lipofectamin to make complex with pre-miRNA oligo - (reply: 1)
515. what is the ORFeome clone? - (reply: 2)
516. ligation with one ristrection site/enzyme - (reply: 3)
517. No amplification during RT-PCR - (reply: 4)
518. rescue cloning of a transposon mutant - (reply: 2)
519. Dephosphorylation - (reply: 7)
520. study of receptors in cell lines - (reply: 1)
521. Mouse genotyping issues - (reply: 1)
522. In-situ hybridization - (reply: 1)
523. His tag introduction in to gene and primer design - (reply: 2)
524. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
525. Principle of silica membrane binding of nucleic acid - (reply: 6)
526. genogroup and genotype - (reply: 2)
527. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
528. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
529. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
530. Beta alanine alternative? - (reply: 2)
531. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
532. lncRNA full-sequence or partial sequence - (reply: 2)
533. Cloning at 6 kb gene - (reply: 3)
534. Trouble with DNA purity (A260/A280) - (reply: 3)
535. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
536. Luciferase assay - extreme variation in RLU - (reply: 1)
537. site-directed mutagenesis - (reply: 20)
538. qPCR detection - (reply: 2)
539. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
540. TCA precipitation - (reply: 1)