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Top : New Forum Archives (2009-): : Molecular-Biology
511. Trouble with DNA purity (A260/A280) - (reply: 3)
512. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
513. Luciferase assay - extreme variation in RLU - (reply: 1)
514. site-directed mutagenesis - (reply: 20)
515. qPCR detection - (reply: 2)
516. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
517. TCA precipitation - (reply: 1)
518. SOC vs TB in transformations - (reply: 1)
519. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
520. Single RE site cloning - (reply: 3)
521. PCR product running on agarose gel - (reply: 32)
522. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
523. PCR amplification with high fidelity enzyme - (reply: 1)
524. Transformation problem - (reply: 5)
525. finding gene sequence to delete - (reply: 4)
526. Blue fluorescent color of agarose gel in UV - (reply: 7)
527. heterologous gene integration into e.coli genome - (reply: 1)
528. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
529. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
530. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
531. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
532. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
533. Continuous growth of E. coli on AMP LB plate - (reply: 2)
534. Plasmid compatibly groups - (reply: 5)
535. too much DNA on agarose gel - (reply: 10)
536. What are the "mammalian vectors N1 and C1"? - (reply: 1)
537. Life before BLAST etc? Help needed... - (reply: 1)
538. How base pairs upstream of gene to include for the promoter? - (reply: 3)
539. Neomycin resistance sequence - (reply: 1)
540. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)