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Top : New Forum Archives (2009-): : Molecular-Biology
511. Mouse genotyping issues - (reply: 1)
512. In-situ hybridization - (reply: 1)
513. His tag introduction in to gene and primer design - (reply: 2)
514. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
515. Principle of silica membrane binding of nucleic acid - (reply: 6)
516. genogroup and genotype - (reply: 2)
517. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
518. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
519. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
520. Beta alanine alternative? - (reply: 2)
521. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
522. lncRNA full-sequence or partial sequence - (reply: 2)
523. Cloning at 6 kb gene - (reply: 3)
524. Trouble with DNA purity (A260/A280) - (reply: 3)
525. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
526. Luciferase assay - extreme variation in RLU - (reply: 1)
527. site-directed mutagenesis - (reply: 20)
528. qPCR detection - (reply: 2)
529. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
530. TCA precipitation - (reply: 1)
531. SOC vs TB in transformations - (reply: 1)
532. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
533. Single RE site cloning - (reply: 3)
534. PCR product running on agarose gel - (reply: 32)
535. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
536. PCR amplification with high fidelity enzyme - (reply: 1)
537. Transformation problem - (reply: 5)
538. finding gene sequence to delete - (reply: 4)
539. Blue fluorescent color of agarose gel in UV - (reply: 7)
540. heterologous gene integration into e.coli genome - (reply: 1)