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Top : New Forum Archives (2009-): : Molecular-Biology
511. Beta alanine alternative? - (reply: 2)
512. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
513. lncRNA full-sequence or partial sequence - (reply: 2)
514. Cloning at 6 kb gene - (reply: 3)
515. Trouble with DNA purity (A260/A280) - (reply: 3)
516. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
517. Luciferase assay - extreme variation in RLU - (reply: 1)
518. site-directed mutagenesis - (reply: 20)
519. qPCR detection - (reply: 2)
520. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
521. TCA precipitation - (reply: 1)
522. SOC vs TB in transformations - (reply: 1)
523. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
524. Single RE site cloning - (reply: 3)
525. PCR product running on agarose gel - (reply: 32)
526. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
527. PCR amplification with high fidelity enzyme - (reply: 1)
528. Transformation problem - (reply: 5)
529. finding gene sequence to delete - (reply: 4)
530. Blue fluorescent color of agarose gel in UV - (reply: 7)
531. heterologous gene integration into e.coli genome - (reply: 1)
532. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
533. Can anyone vouch for a protocol to get good gDNA from fungi? - (reply: 2)
534. Successful Transformation->Getting Colongy--> Getting DNA after miniprep - (reply: 9)
535. Qiagen column eluted DNA gives higher concetration than the original sample - (reply: 10)
536. Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
537. Continuous growth of E. coli on AMP LB plate - (reply: 2)
538. Plasmid compatibly groups - (reply: 5)
539. too much DNA on agarose gel - (reply: 10)
540. What are the "mammalian vectors N1 and C1"? - (reply: 1)