|
1321. Doing PCR on Nebulized DNA - (reply: 2)
1322. e coli plate left too long at 37C - very small clones appeared (reply: 4)
1323. Strand specific probe design for Sindbis virus - (reply: 4)
1324. Genomic DNA purity...??? - strange 260/280 and 260/230 ratios (reply: 7)
1325. Problems with pJET1,2Blunt - (reply: 1)
1326. DH5 Alpha Lambda pir and Conjugation - (reply: 1)
1327. Gel Electrophoresis Smearing Problems - Molecular biology always a mystery! (reply: 4)
1328. Overexpression plasmid is not expressing protein - (reply: 13)
1329. Why shouldn't you clone using expression bacteria - (reply: 6)
1330. Unstable A260/A230 in RNA isolation - (reply: 2)
1331. paraffin from ffpe tissue samples refuse to dissolve in xylene - DNA extraction from ffpe tissue shavings (reply: 2)
1332. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
1333. Radioactive calculation - (reply: 3)
1334. Froze Dig Antibody (Roche) Now What? - (reply: 1)
1335. invitro mutagenesis - (reply: 5)
1336. Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
1337. Extra band on gel after miniprep - (reply: 6)
1338. 3 distinct bands of gDNA on gel - (reply: 2)
1339. PCR Mastermix - (reply: 4)
1340. Unsuccessful self-ligation - (reply: 4)
1341. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
1342. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
1343. DNA extraction from E. coli - solutions needed? (reply: 6)
1344. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1345. Frequency of restriction enzyme sites in a genome - (reply: 5)
1346. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1347. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1348. Internal Control RNA or DNA - (reply: 4)
1349. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1350. RNA extraction - (reply: 3)
1322. e coli plate left too long at 37C - very small clones appeared (reply: 4)
1323. Strand specific probe design for Sindbis virus - (reply: 4)
1324. Genomic DNA purity...??? - strange 260/280 and 260/230 ratios (reply: 7)
1325. Problems with pJET1,2Blunt - (reply: 1)
1326. DH5 Alpha Lambda pir and Conjugation - (reply: 1)
1327. Gel Electrophoresis Smearing Problems - Molecular biology always a mystery! (reply: 4)
1328. Overexpression plasmid is not expressing protein - (reply: 13)
1329. Why shouldn't you clone using expression bacteria - (reply: 6)
1330. Unstable A260/A230 in RNA isolation - (reply: 2)
1331. paraffin from ffpe tissue samples refuse to dissolve in xylene - DNA extraction from ffpe tissue shavings (reply: 2)
1332. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
1333. Radioactive calculation - (reply: 3)
1334. Froze Dig Antibody (Roche) Now What? - (reply: 1)
1335. invitro mutagenesis - (reply: 5)
1336. Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
1337. Extra band on gel after miniprep - (reply: 6)
1338. 3 distinct bands of gDNA on gel - (reply: 2)
1339. PCR Mastermix - (reply: 4)
1340. Unsuccessful self-ligation - (reply: 4)
1341. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
1342. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
1343. DNA extraction from E. coli - solutions needed? (reply: 6)
1344. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1345. Frequency of restriction enzyme sites in a genome - (reply: 5)
1346. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1347. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1348. Internal Control RNA or DNA - (reply: 4)
1349. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1350. RNA extraction - (reply: 3)