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1321. Gel Electrophoresis Smearing Problems - Molecular biology always a mystery! (reply: 4)
1322. Overexpression plasmid is not expressing protein - (reply: 13)
1323. Why shouldn't you clone using expression bacteria - (reply: 6)
1324. Unstable A260/A230 in RNA isolation - (reply: 2)
1325. paraffin from ffpe tissue samples refuse to dissolve in xylene - DNA extraction from ffpe tissue shavings (reply: 2)
1326. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
1327. Radioactive calculation - (reply: 3)
1328. Froze Dig Antibody (Roche) Now What? - (reply: 1)
1329. invitro mutagenesis - (reply: 5)
1330. Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
1331. Extra band on gel after miniprep - (reply: 6)
1332. 3 distinct bands of gDNA on gel - (reply: 2)
1333. PCR Mastermix - (reply: 4)
1334. Unsuccessful self-ligation - (reply: 4)
1335. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
1336. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
1337. DNA extraction from E. coli - solutions needed? (reply: 6)
1338. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1339. Frequency of restriction enzyme sites in a genome - (reply: 5)
1340. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1341. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1342. Internal Control RNA or DNA - (reply: 4)
1343. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1344. RNA extraction - (reply: 3)
1345. mutation using overlapping PCR, what's the success rate? - (reply: 3)
1346. finding exons for a gene, having the coding sequence only - (reply: 4)
1347. colonies without insert!!! - molecular biology (reply: 1)
1348. Effect of phosphoric acid on a kinase assay - (reply: 2)
1349. Proteinase K stock solution - (reply: 3)
1350. Total RNA only produce 1 band in gel - (reply: 2)
1322. Overexpression plasmid is not expressing protein - (reply: 13)
1323. Why shouldn't you clone using expression bacteria - (reply: 6)
1324. Unstable A260/A230 in RNA isolation - (reply: 2)
1325. paraffin from ffpe tissue samples refuse to dissolve in xylene - DNA extraction from ffpe tissue shavings (reply: 2)
1326. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
1327. Radioactive calculation - (reply: 3)
1328. Froze Dig Antibody (Roche) Now What? - (reply: 1)
1329. invitro mutagenesis - (reply: 5)
1330. Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
1331. Extra band on gel after miniprep - (reply: 6)
1332. 3 distinct bands of gDNA on gel - (reply: 2)
1333. PCR Mastermix - (reply: 4)
1334. Unsuccessful self-ligation - (reply: 4)
1335. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
1336. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
1337. DNA extraction from E. coli - solutions needed? (reply: 6)
1338. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1339. Frequency of restriction enzyme sites in a genome - (reply: 5)
1340. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1341. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1342. Internal Control RNA or DNA - (reply: 4)
1343. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1344. RNA extraction - (reply: 3)
1345. mutation using overlapping PCR, what's the success rate? - (reply: 3)
1346. finding exons for a gene, having the coding sequence only - (reply: 4)
1347. colonies without insert!!! - molecular biology (reply: 1)
1348. Effect of phosphoric acid on a kinase assay - (reply: 2)
1349. Proteinase K stock solution - (reply: 3)
1350. Total RNA only produce 1 band in gel - (reply: 2)