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Top : New Forum Archives (2009-): : Molecular-Biology
1321. paraffin from ffpe tissue samples refuse to dissolve in xylene - DNA extraction from ffpe tissue shavings (reply: 2)
1322. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
1323. Radioactive calculation - (reply: 3)
1324. Froze Dig Antibody (Roche) Now What? - (reply: 1)
1325. invitro mutagenesis - (reply: 5)
1326. Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
1327. Extra band on gel after miniprep - (reply: 6)
1328. 3 distinct bands of gDNA on gel - (reply: 2)
1329. PCR Mastermix - (reply: 4)
1330. Unsuccessful self-ligation - (reply: 4)
1331. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
1332. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
1333. DNA extraction from E. coli - solutions needed? (reply: 6)
1334. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1335. Frequency of restriction enzyme sites in a genome - (reply: 5)
1336. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1337. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1338. Internal Control RNA or DNA - (reply: 4)
1339. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1340. RNA extraction - (reply: 3)
1341. mutation using overlapping PCR, what's the success rate? - (reply: 3)
1342. finding exons for a gene, having the coding sequence only - (reply: 4)
1343. colonies without insert!!! - molecular biology (reply: 1)
1344. Effect of phosphoric acid on a kinase assay - (reply: 2)
1345. Proteinase K stock solution - (reply: 3)
1346. Total RNA only produce 1 band in gel - (reply: 2)
1347. Advice on amount of starting template for subcloning - (reply: 3)
1348. New to Gene cloning - Plasmid vector preparation (reply: 2)
1349. PCR for MULTIPLE mutagenesis - (reply: 4)
1350. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)