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1321. Genomic DNA purity...??? - strange 260/280 and 260/230 ratios (reply: 7)
1322. Problems with pJET1,2Blunt - (reply: 1)
1323. DH5 Alpha Lambda pir and Conjugation - (reply: 1)
1324. Gel Electrophoresis Smearing Problems - Molecular biology always a mystery! (reply: 4)
1325. Overexpression plasmid is not expressing protein - (reply: 13)
1326. Why shouldn't you clone using expression bacteria - (reply: 6)
1327. Unstable A260/A230 in RNA isolation - (reply: 2)
1328. paraffin from ffpe tissue samples refuse to dissolve in xylene - DNA extraction from ffpe tissue shavings (reply: 2)
1329. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
1330. Radioactive calculation - (reply: 3)
1331. Froze Dig Antibody (Roche) Now What? - (reply: 1)
1332. invitro mutagenesis - (reply: 5)
1333. Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
1334. Extra band on gel after miniprep - (reply: 6)
1335. 3 distinct bands of gDNA on gel - (reply: 2)
1336. PCR Mastermix - (reply: 4)
1337. Unsuccessful self-ligation - (reply: 4)
1338. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
1339. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
1340. DNA extraction from E. coli - solutions needed? (reply: 6)
1341. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1342. Frequency of restriction enzyme sites in a genome - (reply: 5)
1343. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1344. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1345. Internal Control RNA or DNA - (reply: 4)
1346. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1347. RNA extraction - (reply: 3)
1348. mutation using overlapping PCR, what's the success rate? - (reply: 3)
1349. finding exons for a gene, having the coding sequence only - (reply: 4)
1350. colonies without insert!!! - molecular biology (reply: 1)
1322. Problems with pJET1,2Blunt - (reply: 1)
1323. DH5 Alpha Lambda pir and Conjugation - (reply: 1)
1324. Gel Electrophoresis Smearing Problems - Molecular biology always a mystery! (reply: 4)
1325. Overexpression plasmid is not expressing protein - (reply: 13)
1326. Why shouldn't you clone using expression bacteria - (reply: 6)
1327. Unstable A260/A230 in RNA isolation - (reply: 2)
1328. paraffin from ffpe tissue samples refuse to dissolve in xylene - DNA extraction from ffpe tissue shavings (reply: 2)
1329. Question about RNA extraction & gDNA elimination - can you really eliminate gDNA & how do you like to do it? (reply: 1)
1330. Radioactive calculation - (reply: 3)
1331. Froze Dig Antibody (Roche) Now What? - (reply: 1)
1332. invitro mutagenesis - (reply: 5)
1333. Cleaning up old (not quite ancient) gDNA extractions - (reply: 5)
1334. Extra band on gel after miniprep - (reply: 6)
1335. 3 distinct bands of gDNA on gel - (reply: 2)
1336. PCR Mastermix - (reply: 4)
1337. Unsuccessful self-ligation - (reply: 4)
1338. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
1339. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
1340. DNA extraction from E. coli - solutions needed? (reply: 6)
1341. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1342. Frequency of restriction enzyme sites in a genome - (reply: 5)
1343. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1344. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1345. Internal Control RNA or DNA - (reply: 4)
1346. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1347. RNA extraction - (reply: 3)
1348. mutation using overlapping PCR, what's the success rate? - (reply: 3)
1349. finding exons for a gene, having the coding sequence only - (reply: 4)
1350. colonies without insert!!! - molecular biology (reply: 1)