|
1321. Help! low efficiency of transfection - (reply: 2)
1322. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1323. Cloning before sequencing- is it necessary??? - (reply: 13)
1324. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1325. Guanadine HCl for plasmid miniprep - (reply: 3)
1326. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1327. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1328. 3īRACE problem - only smear - (reply: 4)
1329. salt for DNA precipitation - DNA purification (reply: 3)
1330. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1331. Not getting plasmid from miniprep - (reply: 7)
1332. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1333. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1334. Is this consider smearing? - (reply: 2)
1335. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1336. grow in broth but not on agar... - after transformation (reply: 2)
1337. How many colonies to screen (colony PCR)? - (reply: 4)
1338. Primer Design - aligning sequences for probe/primer design (reply: 2)
1339. Cloning problem - (reply: 2)
1340. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)
1341. 3fragment ligation- troubleshooting - Cloning (reply: 2)
1342. In situ hybridization enigma - (reply: 1)
1343. A cloning nightmare - (reply: 8)
1344. Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
1345. RNA fragmentation - (reply: 3)
1346. RNA extraction - how many cells do I need at least?? (reply: 4)
1347. RNA extraction protocol for small amounts of cells - (reply: 2)
1348. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
1349. DNA sample contaminated with lipids - (reply: 8)
1350. cloning problem - (reply: 5)
1322. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1323. Cloning before sequencing- is it necessary??? - (reply: 13)
1324. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1325. Guanadine HCl for plasmid miniprep - (reply: 3)
1326. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1327. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1328. 3īRACE problem - only smear - (reply: 4)
1329. salt for DNA precipitation - DNA purification (reply: 3)
1330. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1331. Not getting plasmid from miniprep - (reply: 7)
1332. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1333. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1334. Is this consider smearing? - (reply: 2)
1335. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1336. grow in broth but not on agar... - after transformation (reply: 2)
1337. How many colonies to screen (colony PCR)? - (reply: 4)
1338. Primer Design - aligning sequences for probe/primer design (reply: 2)
1339. Cloning problem - (reply: 2)
1340. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)
1341. 3fragment ligation- troubleshooting - Cloning (reply: 2)
1342. In situ hybridization enigma - (reply: 1)
1343. A cloning nightmare - (reply: 8)
1344. Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
1345. RNA fragmentation - (reply: 3)
1346. RNA extraction - how many cells do I need at least?? (reply: 4)
1347. RNA extraction protocol for small amounts of cells - (reply: 2)
1348. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
1349. DNA sample contaminated with lipids - (reply: 8)
1350. cloning problem - (reply: 5)