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Top : New Forum Archives (2009-): : Molecular-Biology
1321. DNA extraction from E. coli - solutions needed? (reply: 6)
1322. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1323. Frequency of restriction enzyme sites in a genome - (reply: 5)
1324. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1325. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1326. Internal Control RNA or DNA - (reply: 4)
1327. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1328. RNA extraction - (reply: 3)
1329. mutation using overlapping PCR, what's the success rate? - (reply: 3)
1330. finding exons for a gene, having the coding sequence only - (reply: 4)
1331. colonies without insert!!! - molecular biology (reply: 1)
1332. Effect of phosphoric acid on a kinase assay - (reply: 2)
1333. Proteinase K stock solution - (reply: 3)
1334. Total RNA only produce 1 band in gel - (reply: 2)
1335. Advice on amount of starting template for subcloning - (reply: 3)
1336. New to Gene cloning - Plasmid vector preparation (reply: 2)
1337. PCR for MULTIPLE mutagenesis - (reply: 4)
1338. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
1339. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
1340. Induction strength : IPTG vs lactose - which one is stronger? (reply: 2)
1341. EMSA - probe disappears with nuclear extract - probe works without nuclear extract... (reply: 1)
1342. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
1343. Digested PCR product migrate slower than uncut - (reply: 8)
1344. PCR question - (reply: 2)
1345. cloning a 34kb insert - please suggest good vectors (reply: 1)
1346. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
1347. Do you know good sequencing companies? - Sanger sequencing (reply: 15)
1348. RNA extraction troubleshooting: low 260/230 - (reply: 1)
1349. Illumina sample prep for Multiplexed sequencing... - How does it work? (reply: 3)
1350. RNA in native gel - (reply: 3)