|
1321. Protein TAG for affinity purification - (reply: 1)
1322. Blunt-Cohesive ligation - Should I change the conditions? (reply: 2)
1323. GFP fused proteins - i'm going to cry... (reply: 3)
1324. what's the meaning? - HEAP (reply: 2)
1325. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
1326. Making gels in advance - (reply: 2)
1327. Phenol-chloroform problem - (reply: 1)
1328. Cloning <100 bp fragments into a plasmid - (reply: 7)
1329. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
1330. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
1331. Amount of DNase to use (Qiagen) - (reply: 7)
1332. COMPLETE RNA degradation - (reply: 18)
1333. HpaI blunt end cloning - (reply: 1)
1334. undesired products in multiplex PCR - (reply: 3)
1335. Generate Supercoil Plasmid - it just a thought... (reply: 5)
1336. help with protein localization study using GFP - (reply: 5)
1337. Does a viral gene have introns? - (reply: 4)
1338. Selecting Lethal Mutants? - (reply: 1)
1339. RNA extraction from stained cells - (reply: 2)
1340. agarose gel electrophoresis - fluorescence which will not migrate from well and does not disappear a (reply: 5)
1341. No band after digestion - (reply: 4)
1342. Problem with ligation - Insert into the "wrong" vector (reply: 11)
1343. finding promoter - (reply: 1)
1344. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
1345. Cloning in pCR2.1 plasmid - (reply: 7)
1346. How long can DNA precipitate at -20? - (reply: 6)
1347. Nucleotides with different font size - (reply: 2)
1348. primer sequence problem - (reply: 2)
1349. Maxiprep using alkaline lysis, very high OD, but low intensity on gel - (reply: 2)
1350. lambda phage library - (reply: 1)
1322. Blunt-Cohesive ligation - Should I change the conditions? (reply: 2)
1323. GFP fused proteins - i'm going to cry... (reply: 3)
1324. what's the meaning? - HEAP (reply: 2)
1325. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
1326. Making gels in advance - (reply: 2)
1327. Phenol-chloroform problem - (reply: 1)
1328. Cloning <100 bp fragments into a plasmid - (reply: 7)
1329. Plasmid miniprep w/ Glass fiber filter plates ???? - Protocol help (reply: 2)
1330. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
1331. Amount of DNase to use (Qiagen) - (reply: 7)
1332. COMPLETE RNA degradation - (reply: 18)
1333. HpaI blunt end cloning - (reply: 1)
1334. undesired products in multiplex PCR - (reply: 3)
1335. Generate Supercoil Plasmid - it just a thought... (reply: 5)
1336. help with protein localization study using GFP - (reply: 5)
1337. Does a viral gene have introns? - (reply: 4)
1338. Selecting Lethal Mutants? - (reply: 1)
1339. RNA extraction from stained cells - (reply: 2)
1340. agarose gel electrophoresis - fluorescence which will not migrate from well and does not disappear a (reply: 5)
1341. No band after digestion - (reply: 4)
1342. Problem with ligation - Insert into the "wrong" vector (reply: 11)
1343. finding promoter - (reply: 1)
1344. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
1345. Cloning in pCR2.1 plasmid - (reply: 7)
1346. How long can DNA precipitate at -20? - (reply: 6)
1347. Nucleotides with different font size - (reply: 2)
1348. primer sequence problem - (reply: 2)
1349. Maxiprep using alkaline lysis, very high OD, but low intensity on gel - (reply: 2)
1350. lambda phage library - (reply: 1)