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451. failure of PCR from yeast genomic DNA - (reply: 1)
452. troubles in DNA digestion - (reply: 7)
453. Best plasmid for GFP + Mammalian Selection - (reply: 1)
454. Transformation /Cloning Problem - (reply: 3)
455. Ligation Problem - (reply: 4)
456. a robust multi-gene cloning method - (reply: 2)
457. I've run out of ideas.... Restriction enzyme digestion - (reply: 6)
458. Colony lift hybridisation positive but clones are negative for insert DNA - (reply: 1)
459. Mortar and Pestle for RNA Extraction - (reply: 1)
460. Any free software for drawing genes? - (reply: 9)
461. TREx pcDNA6/TR - (reply: 2)
462. Plasmid, gene orientation, insert orientation. - (reply: 1)
463. Need stable GFP expression in IPS and embryonic SCs - (reply: 1)
464. good tutorials/protocols/books on gene cloning - (reply: 2)
465. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
466. yeast growth in SC-ura medium - (reply: 1)
467. Optimizing Trizol mRNA extraction - (reply: 3)
468. Expression in trans - (reply: 1)
469. DNA Storage - (reply: 10)
470. RBC LYSIS BUFFER - a good substitute of sucrose? - (reply: 5)
471. Luciferase assay - expected range of raw reading values - (reply: 1)
472. compare between 2 or more genes - (reply: 1)
473. sample preservation for RNA extraction - (reply: 1)
474. Designing primers for the ORFs - (reply: 9)
475. Bioinformatics Software - (reply: 2)
476. Low yields with quiagen midi kit - (reply: 2)
477. Knocking out non-essential gene - (reply: 2)
478. PCR with phophorylated primers - (reply: 1)
479. Nanodrop absorbtion at 290 nm??? - (reply: 1)
480. Plasmid sequencing without primers - (reply: 1)
452. troubles in DNA digestion - (reply: 7)
453. Best plasmid for GFP + Mammalian Selection - (reply: 1)
454. Transformation /Cloning Problem - (reply: 3)
455. Ligation Problem - (reply: 4)
456. a robust multi-gene cloning method - (reply: 2)
457. I've run out of ideas.... Restriction enzyme digestion - (reply: 6)
458. Colony lift hybridisation positive but clones are negative for insert DNA - (reply: 1)
459. Mortar and Pestle for RNA Extraction - (reply: 1)
460. Any free software for drawing genes? - (reply: 9)
461. TREx pcDNA6/TR - (reply: 2)
462. Plasmid, gene orientation, insert orientation. - (reply: 1)
463. Need stable GFP expression in IPS and embryonic SCs - (reply: 1)
464. good tutorials/protocols/books on gene cloning - (reply: 2)
465. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
466. yeast growth in SC-ura medium - (reply: 1)
467. Optimizing Trizol mRNA extraction - (reply: 3)
468. Expression in trans - (reply: 1)
469. DNA Storage - (reply: 10)
470. RBC LYSIS BUFFER - a good substitute of sucrose? - (reply: 5)
471. Luciferase assay - expected range of raw reading values - (reply: 1)
472. compare between 2 or more genes - (reply: 1)
473. sample preservation for RNA extraction - (reply: 1)
474. Designing primers for the ORFs - (reply: 9)
475. Bioinformatics Software - (reply: 2)
476. Low yields with quiagen midi kit - (reply: 2)
477. Knocking out non-essential gene - (reply: 2)
478. PCR with phophorylated primers - (reply: 1)
479. Nanodrop absorbtion at 290 nm??? - (reply: 1)
480. Plasmid sequencing without primers - (reply: 1)