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Top : New Forum Archives (2009-): : Molecular-Biology
451. rescue cloning of a transposon mutant - (reply: 2)
452. Dephosphorylation - (reply: 7)
453. study of receptors in cell lines - (reply: 1)
454. Mouse genotyping issues - (reply: 1)
455. In-situ hybridization - (reply: 1)
456. His tag introduction in to gene and primer design - (reply: 2)
457. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
458. Principle of silica membrane binding of nucleic acid - (reply: 6)
459. genogroup and genotype - (reply: 2)
460. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
461. qPCR multiplex- impact of the fluorophore on probe annealing temperature - (reply: 1)
462. How double stranded DNA becomes single stranded in LAMP assay? - (reply: 3)
463. Beta alanine alternative? - (reply: 2)
464. RNA gel electrophoresis for integrity check - denaturing or non-denaturing?? - (reply: 6)
465. lncRNA full-sequence or partial sequence - (reply: 2)
466. Cloning at 6 kb gene - (reply: 3)
467. Trouble with DNA purity (A260/A280) - (reply: 3)
468. Placental RNA extraction with qiagen rneasy mini plus kit - (reply: 9)
469. Luciferase assay - extreme variation in RLU - (reply: 1)
470. site-directed mutagenesis - (reply: 20)
471. qPCR detection - (reply: 2)
472. Advice needed to subclone big inserts (6 kb) into pET system - (reply: 2)
473. TCA precipitation - (reply: 1)
474. SOC vs TB in transformations - (reply: 1)
475. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
476. Single RE site cloning - (reply: 3)
477. PCR product running on agarose gel - (reply: 32)
478. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
479. PCR amplification with high fidelity enzyme - (reply: 1)
480. Transformation problem - (reply: 5)