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1141. Primer Design - aligning sequences for probe/primer design (reply: 2)
1142. Cloning problem - (reply: 2)
1143. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 1)
1144. 3fragment ligation- troubleshooting - Cloning (reply: 2)
1145. In situ hybridization enigma - (reply: 1)
1146. A cloning nightmare - (reply: 8)
1147. Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
1148. RNA fragmentation - (reply: 3)
1149. RNA extraction - how many cells do I need at least?? (reply: 4)
1150. RNA extraction protocol for small amounts of cells - (reply: 2)
1151. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
1152. DNA sample contaminated with lipids - (reply: 8)
1153. cloning problem - (reply: 5)
1154. colony PCR inconsistent - (reply: 4)
1155. Newbie to Sequencing Question - (reply: 1)
1156. Problem with gel extraction of DNA. - (reply: 1)
1157. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
1158. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
1159. genomic DNA isolation - (reply: 1)
1160. E. coli not growing in liquid cultures :( - (reply: 4)
1161. Tricine SDS-PAGE for plant mitochondria - Membrane proteomics (reply: 1)
1162. DNA product almost 1500 bp - (reply: 1)
1163. Slightly too high 260:280 and 260:230 ratios from gDNA extraction - Should I care? (reply: 2)
1164. Blunt end filling - Blunt end filling (reply: 1)
1165. GHOST in Electrophoresis - (reply: 18)
1166. No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
1167. Transformation background problem - high background in negative control (reply: 9)
1168. Akrylamide Gels - (reply: 2)
1169. Does unautoclaved DEPC inhibit northern blotting? - (reply: 2)
1170. Miniprep and sequencing - (reply: 4)
1142. Cloning problem - (reply: 2)
1143. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 1)
1144. 3fragment ligation- troubleshooting - Cloning (reply: 2)
1145. In situ hybridization enigma - (reply: 1)
1146. A cloning nightmare - (reply: 8)
1147. Microtiter plate directed DNA degradation - Why is DNA on one plate degraded while intact on the next? (reply: 3)
1148. RNA fragmentation - (reply: 3)
1149. RNA extraction - how many cells do I need at least?? (reply: 4)
1150. RNA extraction protocol for small amounts of cells - (reply: 2)
1151. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
1152. DNA sample contaminated with lipids - (reply: 8)
1153. cloning problem - (reply: 5)
1154. colony PCR inconsistent - (reply: 4)
1155. Newbie to Sequencing Question - (reply: 1)
1156. Problem with gel extraction of DNA. - (reply: 1)
1157. How to clone DNA (already cloned in a vector) in to another vector - please help (reply: 20)
1158. plasmid more than recommended for transfection - will it be toxic? (reply: 1)
1159. genomic DNA isolation - (reply: 1)
1160. E. coli not growing in liquid cultures :( - (reply: 4)
1161. Tricine SDS-PAGE for plant mitochondria - Membrane proteomics (reply: 1)
1162. DNA product almost 1500 bp - (reply: 1)
1163. Slightly too high 260:280 and 260:230 ratios from gDNA extraction - Should I care? (reply: 2)
1164. Blunt end filling - Blunt end filling (reply: 1)
1165. GHOST in Electrophoresis - (reply: 18)
1166. No luck with blunt and sticky ligations, need hlep - Prepare for a thorough read (reply: 10)
1167. Transformation background problem - high background in negative control (reply: 9)
1168. Akrylamide Gels - (reply: 2)
1169. Does unautoclaved DEPC inhibit northern blotting? - (reply: 2)
1170. Miniprep and sequencing - (reply: 4)