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Top : New Forum Archives (2009-): : Molecular-Biology
1141. PCR Mastermix - (reply: 4)
1142. Unsuccessful self-ligation - (reply: 4)
1143. retrovirus infection mechanism - retrovirus infection mechanism (reply: 1)
1144. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
1145. DNA extraction from E. coli - solutions needed? (reply: 6)
1146. How to extract a plasmid in a strain carrying two types of plasmids - (reply: 4)
1147. Frequency of restriction enzyme sites in a genome - (reply: 5)
1148. Construction of a N-terminal GFP fusion plasmid - No detectable GFP fluorescence (reply: 2)
1149. Why does T7 RNA transcription start with a GGG? - (reply: 5)
1150. Internal Control RNA or DNA - (reply: 4)
1151. Preparation of phenylmethylsulfonyl fluoride in isopropanol - (reply: 2)
1152. RNA extraction - (reply: 3)
1153. mutation using overlapping PCR, what's the success rate? - (reply: 3)
1154. finding exons for a gene, having the coding sequence only - (reply: 4)
1155. colonies without insert!!! - molecular biology (reply: 1)
1156. Effect of phosphoric acid on a kinase assay - (reply: 2)
1157. Proteinase K stock solution - (reply: 3)
1158. Total RNA only produce 1 band in gel - (reply: 2)
1159. Advice on amount of starting template for subcloning - (reply: 3)
1160. New to Gene cloning - Plasmid vector preparation (reply: 2)
1161. PCR for MULTIPLE mutagenesis - (reply: 4)
1162. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
1163. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
1164. Induction strength : IPTG vs lactose - which one is stronger? (reply: 2)
1165. EMSA - probe disappears with nuclear extract - probe works without nuclear extract... (reply: 1)
1166. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
1167. Digested PCR product migrate slower than uncut - (reply: 8)
1168. PCR question - (reply: 2)
1169. cloning a 34kb insert - please suggest good vectors (reply: 1)
1170. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)