Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
1141. DNA purity - (reply: 1)
1142. Mutagenesis, deletion - what is the best kit/protocol (reply: 6)
1143. Ethidium Bromide Stability - (reply: 1)
1144. alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
1145. primer design - microalgae (reply: 2)
1146. Trouble to UNDERSTAND gene replacement results - (reply: 1)
1147. Would clean dry hot air kill bacteria? - (reply: 6)
1148. Genomic DNA contamination in my RNA sample? - (reply: 8)
1149. Cloning and expressing a mystery 71KDa protein - (reply: 9)
1150. why 12 h culture for plasmid prep? - (reply: 2)
1151. Insert magically shortening??? - Cloning issues (reply: 2)
1152. Transformation issue - (reply: 1)
1153. RNase should be added in which step for gDNA isolation using phenol chloroform m - after lysozyme / preteinase k treatment or after phenol chloroform? (reply: 9)
1154. PCR yield in ng/ul - (reply: 1)
1155. How to add a T7 terminator to a vector? - (reply: 4)
1156. Dna after gel extraction, single or double stranded? - (reply: 1)
1157. Checking RNA on gel after DIG labeling - (reply: 1)
1158. Whole blood RNA and DNA extraction help - Expression analysis and epigenetics (reply: 2)
1159. Plasmid Sequences Mutated :( - Perhaps my insert is too toxic? Recommendations and wisdom welcome. (reply: 6)
1160. DNA-PK inhibitor - Which chemical is the best? (reply: 1)
1161. some mathematics help - meaning of 5.0E+03 (reply: 4)
1162. Storage conditions - (reply: 4)
1163. is it degraded RNA? - I dont get a clear 3-band after electrophoresis (reply: 6)
1164. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
1165. Stability of DNA at RT - (reply: 1)
1166. yeast expression (gal1-promotor) - expression in glucose?? (reply: 1)
1167. TRIZol Reagent additional reagents & protocol clarifications - (reply: 4)
1168. Is it possible to islate strong negative charged protein - (reply: 1)
1169. DIG southern blot - (reply: 2)
1170. Real time PCR in the presence of heme - (reply: 3)