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Top : New Forum Archives (2009-): : Molecular-Biology
2341. Transcription of double blunt end Clone - (reply: 2)
2342. PCR with genomic dna - (reply: 9)
2343. mutagenesis - (reply: 1)
2344. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
2345. PCR with long primers - (reply: 3)
2346. Need Help: Bacterial gene deletion- sacB sucrose resistance - (reply: 31)
2347. Gateway cloning - (reply: 8)
2348. RNA Isolation - RNA isolation from human cell lines (reply: 2)
2349. Which red fluorescence is better? - mCherry of dsRed (reply: 2)
2350. Finding corresponding amino acid in DNA sequence - (reply: 1)
2351. Buffer for 5 Kb DNA fragments - (reply: 2)
2352. Pooling doesn't work - make up rna amount of 10ug (reply: 3)
2353. Western Blot for Bacteria - Loading Control (reply: 1)
2354. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
2355. Southern blotting - copy number determination (reply: 1)
2356. RNase: RNA ratio - making dsRNA (reply: 1)
2357. I hate my Klenow! - (reply: 9)
2358. Tris Base Buffer is the same that Trizma??No I think.. - Buffers:Differences between Tris Base and Trizma.. (reply: 3)
2359. Sequencing doubts - (reply: 3)
2360. Help with Northern Blotting - (reply: 4)
2361. ELISA - how can i get Mab for NS1? (reply: 1)
2362. 0.5% crystal violet - how to make (reply: 2)
2363. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
2364. cDNA 260/280 ratio - (reply: 3)
2365. Amino acid substitution a problem? - Phenylalanine to leucine (reply: 3)
2366. What would happen? sequencing question - (reply: 2)
2367. Chemical transfection with Calcium Chloride - anyone used this method and made the 2X HBS? (reply: 6)
2368. About reporting a new splice variant in NCBI - (reply: 1)
2369. foci formation assay - crystal violet (reply: 1)
2370. In situ hybridization - zebrafish (reply: 1)