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Top : New Forum Archives (2009-): : Molecular-Biology
2341. PCR with genomic dna - (reply: 9)
2342. mutagenesis - (reply: 1)
2343. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
2344. PCR with long primers - (reply: 3)
2345. Need Help: Bacterial gene deletion- sacB sucrose resistance - (reply: 31)
2346. Gateway cloning - (reply: 8)
2347. RNA Isolation - RNA isolation from human cell lines (reply: 2)
2348. Which red fluorescence is better? - mCherry of dsRed (reply: 2)
2349. Finding corresponding amino acid in DNA sequence - (reply: 1)
2350. Buffer for 5 Kb DNA fragments - (reply: 2)
2351. Pooling doesn't work - make up rna amount of 10ug (reply: 3)
2352. Western Blot for Bacteria - Loading Control (reply: 1)
2353. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
2354. Southern blotting - copy number determination (reply: 1)
2355. RNase: RNA ratio - making dsRNA (reply: 1)
2356. I hate my Klenow! - (reply: 9)
2357. Tris Base Buffer is the same that Trizma??No I think.. - Buffers:Differences between Tris Base and Trizma.. (reply: 3)
2358. Sequencing doubts - (reply: 3)
2359. Help with Northern Blotting - (reply: 4)
2360. ELISA - how can i get Mab for NS1? (reply: 1)
2361. 0.5% crystal violet - how to make (reply: 2)
2362. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
2363. cDNA 260/280 ratio - (reply: 3)
2364. Amino acid substitution a problem? - Phenylalanine to leucine (reply: 3)
2365. What would happen? sequencing question - (reply: 2)
2366. Chemical transfection with Calcium Chloride - anyone used this method and made the 2X HBS? (reply: 6)
2367. About reporting a new splice variant in NCBI - (reply: 1)
2368. foci formation assay - crystal violet (reply: 1)
2369. In situ hybridization - zebrafish (reply: 1)
2370. transient transfection - (reply: 2)