Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
2311. NEB Restriction Enzymes and Buffer - Restriction (reply: 13)
2312. rna isolation - (reply: 1)
2313. DNA extraction with expired kit? - (reply: 1)
2314. Transcription of double blunt end Clone - (reply: 2)
2315. PCR with genomic dna - (reply: 9)
2316. mutagenesis - (reply: 1)
2317. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
2318. PCR with long primers - (reply: 3)
2319. Need Help: Bacterial gene deletion- sacB sucrose resistance - (reply: 31)
2320. Gateway cloning - (reply: 8)
2321. RNA Isolation - RNA isolation from human cell lines (reply: 2)
2322. Which red fluorescence is better? - mCherry of dsRed (reply: 2)
2323. Finding corresponding amino acid in DNA sequence - (reply: 1)
2324. Buffer for 5 Kb DNA fragments - (reply: 2)
2325. Pooling doesn't work - make up rna amount of 10ug (reply: 3)
2326. Western Blot for Bacteria - Loading Control (reply: 1)
2327. uggly, non-focus and diffuse bands in my Western.. - Can you see my image of my Western to LC3? (reply: 1)
2328. Southern blotting - copy number determination (reply: 1)
2329. RNase: RNA ratio - making dsRNA (reply: 1)
2330. I hate my Klenow! - (reply: 9)
2331. Tris Base Buffer is the same that Trizma??No I think.. - Buffers:Differences between Tris Base and Trizma.. (reply: 3)
2332. Sequencing doubts - (reply: 3)
2333. Help with Northern Blotting - (reply: 4)
2334. ELISA - how can i get Mab for NS1? (reply: 1)
2335. 0.5% crystal violet - how to make (reply: 2)
2336. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
2337. cDNA 260/280 ratio - (reply: 3)
2338. Amino acid substitution a problem? - Phenylalanine to leucine (reply: 3)
2339. What would happen? sequencing question - (reply: 2)
2340. Chemical transfection with Calcium Chloride - anyone used this method and made the 2X HBS? (reply: 6)