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2311. Site directed mutagenesis - (reply: 2)
2312. Two bands for p53 on Western Blot - (reply: 7)
2313. Mutation during transformation/maxiprep - (reply: 1)
2314. beta actin - loading control (reply: 1)
2315. Tranformation cell harvesting - (reply: 5)
2316. Sequencing - identifying backbone and deciphering insert information - (reply: 8)
2317. dna quantification and quality assesement - abs 260nm / abs 280 nm <0.5 (reply: 2)
2318. Best plasmid miniprep? - (reply: 13)
2319. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
2320. Volume of DNA to add to PCR Master Mix? - (reply: 1)
2321. PCR components storage - (reply: 3)
2322. problem in chemiluminiscent EMSA PAGE running - (reply: 2)
2323. Alkaline phosphatase - (reply: 5)
2324. resolving a 100bp difference at around 1.2kb (AGE) - (reply: 2)
2325. RNA, mRNA, sequencing... HELP - (reply: 2)
2326. No PCR bands with some templates - (reply: 5)
2327. RNA Purification - After DNase (reply: 3)
2328. How To Fix NaOH/Tris DNA Digest Error? - (reply: 1)
2329. Taq Dilution - (reply: 8)
2330. Homogenation of Mouse Spinal Cord Tissue for RNA Extraction - Most effective way to do it? (reply: 3)
2331. PCR product purification - (reply: 3)
2332. EMSA - Band doesn't shift with poly dI-dC - (reply: 2)
2333. Temp Gradient PCR - (reply: 2)
2334. BamHI and HindIII double digest - driving me mad... (reply: 1)
2335. NEB Restriction Enzymes and Buffer - Restriction (reply: 13)
2336. rna isolation - (reply: 1)
2337. DNA extraction with expired kit? - (reply: 1)
2338. Transcription of double blunt end Clone - (reply: 2)
2339. PCR with genomic dna - (reply: 9)
2340. mutagenesis - (reply: 1)