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Top : New Forum Archives (2009-): : Molecular-Biology
2011. dilution problem - dilution problem (reply: 1)
2012. How do i increase my rna yield - How do i increase my rna yield (reply: 3)
2013. sequencing with forward/reverse primers - (reply: 7)
2014. can klenow fragment be used to remove the 3' overhangs? - (reply: 1)
2015. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
2016. Viewing Gels - (reply: 3)
2017. ccdB survival E. coli transformation - (reply: 2)
2018. how to get higher RNA purity? - (reply: 3)
2019. Snap freezing worm samples for RNA prep - is it essential? (reply: 1)
2020. Adding A overhangs - primer design implications? - (reply: 1)
2021. I can't resolve 2 proteins (LC3-I and II) in Tricine gels - (reply: 5)
2022. Is an enzyme protein of 1100 aa too big to be expressed in E coli - (reply: 4)
2023. Fungal DNA Isolation - Fungal DNA isolation without liquid nitrogen (reply: 1)
2024. Translational Efficiancy - (reply: 1)
2025. Electroporation strange problem - (reply: 1)
2026. reverse transcriptase and cDNA - (reply: 1)
2027. gene expression, reverse transcription on total RNA or mRNA? - (reply: 3)
2028. stable cell line - (reply: 2)
2029. Site-directed mutagenesis (repeat oligo insert) - Strange mutagenesis reults! (reply: 10)
2030. Low RNA yield after extraction by RNA blood mini kit. - (reply: 2)
2031. Restriction Enzymes - Enzymes from different companies work together? (reply: 2)
2032. Plant RNA Isolation Aid - (reply: 3)
2033. best plasmid purification kit for transfection - (reply: 1)
2034. insert sequence in clone - (reply: 6)
2035. Question about Plasmid Isolation Protocol containing Phenol/Chloroform PEG - (reply: 4)
2036. how long is carb stable in liquid broth at 37C - I need longer incubation times of bacteria because of the transformant (reply: 1)
2037. Blotting technique principle - (reply: 1)
2038. any one tried in-vitro expression system ? - Roche is the best ? (reply: 1)
2039. Taq at 2-8 - (reply: 4)
2040. cloning in pET 30 B - (reply: 1)