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Top : New Forum Archives (2009-): : Molecular-Biology
2011. have problems with electroporation transformation of PCP20 into BL21 de3 - (reply: 2)
2012. subcloning problem - inframe fusion (reply: 1)
2013. SSCP - (reply: 2)
2014. OD reading - (reply: 1)
2015. E coli transformation - (reply: 8)
2016. RNAsin overdose! - (reply: 1)
2017. Gel loading dye : sample ratio?? - have to follow strictly? (reply: 6)
2018. how to intentionally alter gene sequence - (reply: 1)
2019. molecular biology - (reply: 2)
2020. How to remove diphosphate from in vitro transcribed RNA? - (reply: 1)
2021. Experience with YM-30 microcon columns from Millipore - (reply: 2)
2022. DNA stains - pronunciation question (reply: 3)
2023. Floating white pellet in miniprep - (reply: 2)
2024. Gel electrophoresis problem - (reply: 7)
2025. Making competent cell from freeze-thawed stock - (reply: 2)
2026. Fluorescent measurements-AMCA - (reply: 4)
2027. northern prob - (reply: 4)
2028. dna smear in electrophoresis - (reply: 6)
2029. does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
2030. Reverse-transcription of UV-damaged RNA - (reply: 3)
2031. How could I express two genes in opposite orientation - (reply: 2)
2032. shRNA oligo annealing problem - (reply: 5)
2033. Ligated Vector Age - When is a ligated vector too old to use? (reply: 1)
2034. GFP insert - trying to insert GFP in a vector (reply: 1)
2035. PCR problems on high GC content gene - Trouble with Colony PCR of TOP10 transformants w/ TOPO-vector (reply: 4)
2036. miRNA northern - (reply: 1)
2037. Double digestion and cloning - (reply: 2)
2038. Plasmid problem - From PCR product (reply: 7)
2039. cloning of long fragment - (reply: 1)
2040. how to find out the level of expression among different transcripts of the same - (reply: 1)