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Top : New Forum Archives (2009-): : Molecular-Biology
1531. regenerate qiagen midi columns - how to reuse the midiprep DNA columns (reply: 3)
1532. Is there any software that can be sued to design degenerate primers - (reply: 1)
1533. Primer Decontamination - (reply: 2)
1534. PGem Easy Vector System: ampicillin conc and plasmid length problem - (reply: 10)
1535. mRNA preparation from total RNA - a role of poly A in mRNA preparation from total RNA (reply: 2)
1536. How long is "overnight"? - (reply: 3)
1537. Trizol stability - (reply: 1)
1538. DNA stuck in gel well - some samples are stuck in the agarose-gel well and don't migrate (reply: 4)
1539. Tube Openers - (reply: 3)
1540. taq man probes non specific? - (reply: 1)
1541. Help! low efficiency of transfection - (reply: 2)
1542. 3 ways ligation for cloning - 2x 210bp fragments and pET 15-b (reply: 2)
1543. Cloning before sequencing- is it necessary??? - (reply: 13)
1544. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
1545. Guanadine HCl for plasmid miniprep - (reply: 3)
1546. Generating Standard for qPCR - PCR vs plasmid DNA (reply: 1)
1547. Ligation problem: clones survive selection but nothing in there? - ligation, cloning (reply: 24)
1548. 3īRACE problem - only smear - (reply: 4)
1549. salt for DNA precipitation - DNA purification (reply: 3)
1550. Conditional knockout mated to cre not deleting target exon - (reply: 8)
1551. Not getting plasmid from miniprep - (reply: 7)
1552. DNA sequencing question help - make up sequencing mastermix (reply: 1)
1553. Why isnt Phenol miscible in Tris Buffer? - Isolation of Genomic DNA (reply: 1)
1554. Is this consider smearing? - (reply: 2)
1555. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
1556. grow in broth but not on agar... - after transformation (reply: 2)
1557. How many colonies to screen (colony PCR)? - (reply: 4)
1558. Primer Design - aligning sequences for probe/primer design (reply: 2)
1559. Cloning problem - (reply: 2)
1560. Lentivirus expression, no target protein - pHAGE-CMV-MCS-IZsGreen insert with S100B (reply: 2)